{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T15:08:14Z","timestamp":1753888094455,"version":"3.41.2"},"reference-count":36,"publisher":"FapUNIFESP (SciELO)","license":[{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Braz. J. Biol."],"abstract":"<jats:p>Abstract The tambaqui (Colossoma macropomum) is a species of great economic importance for fish farming in the Brazilian Amazon, and acanthocephaliasis caused by Neoechinorhynchus buttnerae (Golvan 1956) represents an obstacle to its production due to it causing severe morphological damage to the intestinal mucosa, thus impairing the absorption of nutrients and causing weight loss in the fish. Therefore, the establishment of in vitro protocols for evaluation of anthelmintic drugs is the first step to development of effective measures for in vivo control of this endoparasite. The present study evaluated the in vitro survival of N. buttnerae maintained in Eagle\u2019s minimum essential medium under different culture conditions. Three assays were carried out to evaluate whether temperature, supplementation with the antibiotics penicillin and streptomycin, and culture medium replacement or no replacement would influence the motility and morphology of the acanthocephalans. The results of the Kaplan-Meier analysis indicated that the use of culture in minimum essential medium together with penicillin and streptomycin prolonged the parasite\u2019s survival when kept at temperatures of 24 \u00b0C or 28 \u00b0C. We describe herein for first time an alternative protocol that is ideal for the in vitro culture of N. buttnerae. As such, this protocol ensures greater reliability in further in vitro studies with N. buttnerae.<\/jats:p>","DOI":"10.1590\/1519-6984.275748","type":"journal-article","created":{"date-parts":[[2023,11,13]],"date-time":"2023-11-13T11:19:51Z","timestamp":1699874391000},"source":"Crossref","is-referenced-by-count":0,"title":["In vitro culture and morphology of Neoechinorhynchus buttnerae (Eoacanthocephala: Neoechinorhynchidae) collected from the intestine of tambaqui (Colossoma macropomum) farmed in the Brazilian Amazon"],"prefix":"10.1590","volume":"83","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8408-8761","authenticated-orcid":false,"given":"M. I. B.","family":"Oliveira","sequence":"first","affiliation":[{"name":"Universidade Federal do Amazonas,  Brasil; Universidade Federal do Amazonas,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7554-9514","authenticated-orcid":false,"given":"C.","family":"Majolo","sequence":"additional","affiliation":[{"name":"Embrapa Amaz\u00f4nia Ocidental,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3107-681X","authenticated-orcid":false,"given":"F. A.","family":"Sebasti\u00e3o","sequence":"additional","affiliation":[{"name":"Universidade Federal do Amazonas,  Brasil; Embrapa Amaz\u00f4nia Ocidental,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8376-1846","authenticated-orcid":false,"given":"M.","family":"Tavares-Dias","sequence":"additional","affiliation":[{"name":"Embrapa Amap\u00e1,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1753-8742","authenticated-orcid":false,"given":"F. R.","family":"Brand\u00e3o","sequence":"additional","affiliation":[{"name":"Universidade Federal do Amazonas,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9170-9725","authenticated-orcid":false,"given":"E. C.","family":"Chagas","sequence":"additional","affiliation":[{"name":"Embrapa Amaz\u00f4nia Ocidental,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","published-online":{"date-parts":[[2023]]},"reference":[{"issue":"2","key":"ref1","doi-asserted-by":"crossref","first-page":"80","DOI":"10.4103\/2229-5070.138534","article-title":"Cultivation of parasites","volume":"4","author":"AHMED N.H.","year":"2014","journal-title":"Tropical Parasitology"},{"year":"1998","edition":"20th","series-title":"Standard methods for the examination of water and wastewater.","key":"ref2"},{"issue":"4","key":"ref3","doi-asserted-by":"crossref","first-page":"747","DOI":"10.1017\/S0031182000086315","article-title":"The ecology of Echinorhynchus truttae Schrank, 1788 (Acanthocephala) in a trout stream in North Wales","volume":"55","author":"AWACHIE J.","year":"1965","journal-title":"Parasitology"},{"year":"2018","series-title":"Diretriz da pr\u00e1tica de eutan\u00e1sia em animais inclu\u00eddos em atividades de ensino ou de pesquisa cient\u00edfica","key":"ref4"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"169","DOI":"10.3354\/dao024169","article-title":"Temperature, pH and bile dependent in vitro cultivation of Hexamita salmonis from rainbow trout Oncorhynchus mykiss intestine","volume":"24","author":"BUCHMANN K.","year":"1996","journal-title":"Diseases of Aquatic Organisms"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1016\/j.vetpar.2018.12.011","article-title":"Survival of Taenia saginata eggs under different environmental conditions","volume":"266","author":"BUCUR I.","year":"2019","journal-title":"Veterinary Parasitology"},{"issue":"2","key":"ref7","doi-asserted-by":"crossref","first-page":"225","DOI":"10.2307\/3283751","article-title":"In vitro culture of equine strongylidae to the fourth larval stage in a cell-free medium","volume":"80","author":"CHAPMAN M.","year":"1994","journal-title":"The Journal of Parasitology"},{"issue":"4","key":"ref8","doi-asserted-by":"crossref","first-page":"562","DOI":"10.1590\/s1984-296120180079","article-title":"In vitro culture of Neoechinorhynchus buttnerae (Acanthocephala: Neoechinorhynchidae): Influence of temperature and culture media. 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Brasil","volume":"27","author":"COSTA C.M.S.","year":"2018","journal-title":"Revista Brasileira de Parasitologia Veterin\u00e1ria"},{"issue":"6","key":"ref9","doi-asserted-by":"crossref","first-page":"1049","DOI":"10.1111\/jai.12865","article-title":"Seasonality affects the parasitism levels in two fish species in the eastern Amazon region","volume":"31","author":"DIAS M.K.R.","year":"2015","journal-title":"Journal of Applied Ichthyology"},{"key":"ref10","first-page":"131","article-title":"Studies on in vitro survival of Acanthocephala","volume":"29","author":"DUNAGAN T.","year":"1962","journal-title":"Proceedings of the Helminthological Society of Washington"},{"issue":"6","key":"ref11","doi-asserted-by":"crossref","first-page":"1099","DOI":"10.2307\/3282287","article-title":"Population dynamics of Pomphorhynchus bulbocolli in Gammarus pseudolimnaeus.","volume":"73","author":"GLEASON L.N.","year":"1987","journal-title":"The Journal of 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conditions for the maintenance of Onchocerca gutturosa adult worms in vitro.","volume":"60","author":"TOWNSON S.","year":"1986","journal-title":"Journal of Helminthology"},{"issue":"4","key":"ref30","doi-asserted-by":"crossref","first-page":"309","DOI":"10.3347\/kjp.2012.50.4.309","article-title":"In vitro maintenance of Clonorchis sinensis adult worms","volume":"50","author":"UDDIN M.H.","year":"2012","journal-title":"Korean Journal of Parasitology"},{"issue":"1","key":"ref31","doi-asserted-by":"crossref","DOI":"10.1088\/1755-1315\/441\/1\/012020","article-title":"The effect of temperature, salinity and antimicrobial agent on growth and viability of Aeromonas hydrophila.","volume":"441","author":"ULKHAQ M.","year":"2020","journal-title":"IOP Conference Series. Earth and Environmental Science"},{"issue":"3","key":"ref32","doi-asserted-by":"crossref","first-page":"1360","DOI":"10.1111\/raq.12386","article-title":"Challenges in the control of acanthocephalosis in aquaculture: special emphasis on Neoechinorhynchus buttnerae Rev","volume":"12","author":"VALLAD\u00c3O G.M.R.","year":"2020","journal-title":"Reviews in Aquaculture"},{"year":"2019","author":"WOYN\u00c1ROVICH A.","series-title":"Field guide to the culture of tambaqui (Colossoma macropomum, Cuvier, 1816).","key":"ref33"},{"issue":"2","key":"ref34","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1002\/rmb2.12024","article-title":"Animal\u2010cell culture media: history, characteristics, and current issues","volume":"16","author":"YAO T.","year":"2017","journal-title":"Reproductive Medicine and Biology"},{"key":"ref35","series-title":"Progress of medical parasitology in Japan.","first-page":"211","volume-title":"In vitro cultivation of parasitic helminths.","author":"YASURAOKA K.","year":"2003"},{"issue":"1","key":"ref36","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1186\/s13071-018-2852-2","article-title":"Evaluation of in vitro culture systems for the maintenance of microfilariae and infective larvae of Loa loa.","volume":"11","author":"ZOFOU D.","year":"2018","journal-title":"Parasites & Vectors"}],"container-title":["Brazilian Journal of Biology"],"original-title":["Cultivo in vitro e morfologia de Neoechinorhynchus buttnerae (Eoacanthocephala: Neoechinorhynchidae) coletados do intestino de tambaqui (Colossoma macropomum) cultivado na Amaz\u00f4nia brasileira"],"link":[{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_pdf&pid=S1519-69842023000101012&tlng=en","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,13]],"date-time":"2023-11-13T11:20:05Z","timestamp":1699874405000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_arttext&pid=S1519-69842023000101012&tlng=en"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023]]},"references-count":36,"alternative-id":["S1519-69842023000101012"],"URL":"https:\/\/doi.org\/10.1590\/1519-6984.275748","relation":{},"ISSN":["1678-4375","1519-6984"],"issn-type":[{"type":"electronic","value":"1678-4375"},{"type":"print","value":"1519-6984"}],"subject":[],"published":{"date-parts":[[2023]]},"article-number":"e275748"}}