{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T22:08:59Z","timestamp":1790028539951,"version":"4.0.1"},"reference-count":49,"publisher":"FapUNIFESP (SciELO)","license":[{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Braz. J. Biol."],"abstract":"<jats:p>Abstract The water deficit in particular, reduces the productivity of vegetable crops. To minimize these harmful effects on agriculture, several agronomic and physiological practices are being studied, such as the use of bacteria and water stress attenuators, such as brassinosteroids. Considering the socioeconomic relevance of corn culture and its sensitivity when exposed to water deficit, the objective of the present study was to evaluate the action of brassinosteroids and azospirillum on nitrogen metabolism in corn plants subjected to water stress conditions. The experiment was carried out in a greenhouse, in a period of 47 days, with corn plants, using the hybrid K9606 VIP3. The design was completely randomized, in a 2x2x3 factorial scheme, with six replications. The first factor corresponds to two water regimes (presence and absence of water deficit). The second corresponds to inoculation via seed of Azospirillum brasiliense and absence of inoculation. And the third corresponds to the application of three concentrations of brassinosteroids (0, 0.3 and 0.6 \u03bcM). Were determined Nitrate; nitrate reductase; free ammonium; total soluble aminoacids; soluble proteins; proline; glycine betaine and glutamine synthetase. The lack of water in plants provided a reduction in the protein and nitrate reductase contents, in leaves and roots. For ammonium, plants with water deficit inoculated at a concentration of 0.3 \u03bcM, obtained an increase of 7.16 (70.26%) and 13.89 (77.04%) mmol NH4 + .Kg-1. DM (Dry mass) on the leaf and root respectively. The water deficit in the soil provided significant increases in the concentrations of glycine betaine, nitrate, proline and aminoacids, both in the leaves and in the roots of the corn plants. On the other hand, the contents of glutamine synthetase had a reduction in both leaves and roots.<\/jats:p>","DOI":"10.1590\/1519-6984.276264","type":"journal-article","created":{"date-parts":[[2023,11,3]],"date-time":"2023-11-03T13:44:07Z","timestamp":1699019047000},"source":"Crossref","is-referenced-by-count":5,"title":["Nitrogen metabolism in maize plants submitted to drought, brassinosteroids and azospirillum"],"prefix":"10.1590","volume":"83","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7938-8436","authenticated-orcid":false,"given":"L. C.","family":"Souza","sequence":"first","affiliation":[{"name":"Universidade Federal Rural da Amaz\u00f4nia,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0572-1731","authenticated-orcid":false,"given":"G. G. T. N.","family":"Monteiro","sequence":"additional","affiliation":[{"name":"Universidade de S\u00e3o Paulo,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-6823-480X","authenticated-orcid":false,"given":"R. K. M.","family":"Marinho","sequence":"additional","affiliation":[{"name":"Universidade Federal Rural da Amaz\u00f4nia,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2234-9313","authenticated-orcid":false,"given":"E. F. L.","family":"Souza","sequence":"additional","affiliation":[{"name":"Universidade Federal Rural da Amaz\u00f4nia,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7251-6562","authenticated-orcid":false,"given":"S. C. F.","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Universidade Federal Rural da Amaz\u00f4nia,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-5503-8786","authenticated-orcid":false,"given":"A. C. S.","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Universidade Federal Rural da Amaz\u00f4nia,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6070-0549","authenticated-orcid":false,"given":"C. F.","family":"Oliveira Neto","sequence":"additional","affiliation":[{"name":"Universidade Federal Rural da Amaz\u00f4nia,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5079-3980","authenticated-orcid":false,"given":"R. S.","family":"Okumura","sequence":"additional","affiliation":[{"name":"Universidade Federal Rural da Amaz\u00f4nia,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4500-4035","authenticated-orcid":false,"given":"L. C.","family":"Souza","sequence":"additional","affiliation":[{"name":"Universidade Federal do Maranh\u00e3o,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"530","published-online":{"date-parts":[[2023]]},"reference":[{"issue":"1","key":"ref1","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1093\/jxb\/ers323","article-title":"Role of brassinosteroids in alleviation of phenanthrene-cadmium co-contamination-induced pliotosynthetic inhibition and oxidative stress in tomato","volume":"64","author":"AHAMMED G.J.","year":"2013","journal-title":"Journal of Experimental Botany"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1016\/B978-0-12-387689-8.00002-3","article-title":"Drought tolerance: roles of organic osmolytes, growth regulators, and mineral nutrients","volume":"111","author":"ASHRAF M.","year":"2011","journal-title":"Advances in 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Physiological characterization of plants overexpressing the two enzyme subunits individually or simultaneously","volume":"54","author":"TERC\u00c9-LAFORGUE T.","year":"2013","journal-title":"Plant & Cell Physiology"},{"issue":"4","key":"ref46","first-page":"1","article-title":"Application of brassinolide mitigates saline stress of certain metabolites of sorghum grown in Karaikal","volume":"4","author":"VARDHINI B.V.","year":"2012","journal-title":"Journal of Phytology"},{"issue":"8","key":"ref47","doi-asserted-by":"crossref","first-page":"971","DOI":"10.1021\/ac60252a045","article-title":"Phenol hipochlorite reaction for determination of ammonia","volume":"39","author":"WEATHERBURN M.W.","year":"1967","journal-title":"Analytical Chemistry"},{"issue":"1","key":"ref48","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1016\/j.molp.2015.12.003","article-title":"Brassinosteroids regulate root growth, development, and symbiose","volume":"9","author":"WEI Z.","year":"2016","journal-title":"Molecular Plant"},{"issue":"2","key":"ref49","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1093\/jxb\/erl227","article-title":"Are source and sink strengths genetically linked in maize plants subjected to water d\u00e9ficit? 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