{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,10]],"date-time":"2026-01-10T02:59:33Z","timestamp":1768013973753,"version":"3.49.0"},"reference-count":64,"publisher":"FapUNIFESP (SciELO)","license":[{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Braz. J. Biol."],"abstract":"<jats:p>Abstract The objective of the study was to carry out phytochemical prospection through colorimetric tests to determine the groups of secondary metabolites and also to determine the total content of phenolic compounds (TPC) present in plant extracts methanol (ME), ethyl acetate (EAE), hexane (HE) and dichloromethane (DE) from the leaves of Myrsine umbellata, as well as to investigate the antimicrobial activity against twelve standard ATCC strains by the broth microdilution technique; the antioxidant potential by the DPPH method and the ABTS method and the antibiofilm potential on the biofilm biomass of standard bacteria by the crystal violet technique and tetrazolium salt reduction (MTT) assay. Phytochemical prospection detected the presence of saponins, steroids, alkaloids, anthocyanins, anthocyanidins, flavonoids, and tannins. The results of the quantitative phytochemical estimation revealed a higher content of total phenolics in DE (280.24 \u00b1 0.037 \u00b5M GAE g ext. -1) followed by ME (159.01 \u00b1 0.031 \u00b5M GAE g ext. -1). The ME showed the best biological activities when compared to the other extracts tested. We observed antimicrobial activity against Gram-positive Staphylococcus epidermidis strain (MIC 3.12 and MBC 6.25), antioxidant percentage of 92.58% against the DPPH radical and 420.31 \u00b5M Trolox g ext. -1 against the ABTS radical, finally showed antibiofilm action against Gram-positive strain Staphylococcus aureus, with eradication of the biomass in 92.58%. The results suggest that EM from M. umbellata represents an alternative source of plant bioactives for the development of natura products.<\/jats:p>","DOI":"10.1590\/1519-6984.276871","type":"journal-article","created":{"date-parts":[[2024,3,4]],"date-time":"2024-03-04T10:54:14Z","timestamp":1709549654000},"source":"Crossref","is-referenced-by-count":2,"title":["Investigation of phytochemical composition and bioactivity evaluation of extracts from Myrsine umbellata Mart."],"prefix":"10.1590","volume":"84","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8185-3790","authenticated-orcid":false,"given":"L. V.","family":"Laskoski","sequence":"first","affiliation":[{"name":"Universidade Estadual do Oeste do Paran\u00e1,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1699-9643","authenticated-orcid":false,"given":"J. M.","family":"Batista","sequence":"additional","affiliation":[{"name":"Universidade Estadual do Oeste do Paran\u00e1,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5956-7210","authenticated-orcid":false,"given":"D. M.","family":"Bandeira","sequence":"additional","affiliation":[{"name":"Universidade Estadual do Oeste do Paran\u00e1,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4526-0060","authenticated-orcid":false,"given":"J. M.","family":"Corr\u00eaa","sequence":"additional","affiliation":[{"name":"Universidade Estadual do Oeste do Paran\u00e1,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8348-0649","authenticated-orcid":false,"given":"J.","family":"Rosset","sequence":"additional","affiliation":[{"name":"Universidade Estadual do Oeste do Paran\u00e1,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9804-3476","authenticated-orcid":false,"given":"L. H. S. M.","family":"Concei\u00e7\u00e3o","sequence":"additional","affiliation":[{"name":"Universidade Estadual do Oeste do Paran\u00e1,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0486-8486","authenticated-orcid":false,"given":"F. G. 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