{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,26]],"date-time":"2026-04-26T06:43:38Z","timestamp":1777185818033,"version":"3.51.4"},"reference-count":35,"publisher":"FapUNIFESP (SciELO)","license":[{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Braz. J. Biol."],"abstract":"<jats:p>Abstract Leishmaniasis is a vector-transmitted zoonosis caused by different species of the genus Leishmania, with a wide clinical spectrum. It is a public health problem aggravated by a series of limitations regarding treatment. In the search for new therapeutic alternatives, scorpion venoms are a source of multifunctional molecules that act against the natural resistance of pathogens. This work evaluated the antileishmanial potential of Brotheas amazonicus and Tityus metuendus venoms against the promastigote forms of Leishmania amazonensis e Leishmania guyanensis. The venoms of B. amazonicus and T. metuendus were evaluated for their constituents using Fourier Transform Infrared (FTIR). Growth inhibition and death of promastigotes were evaluated in the presence of diferente crude venom concentrations (100 \u00b5g\/mL, 50 \u00b5g\/mL, 10 \u00b5g\/mL, 1 \u00b5g\/mL) after one hour of incubation at 25 \u00b0C. The FTIR spectra of both venoms exhibited bands in approximate regions, revealing that both exhibit similar functional groups. Crude venom from both scorpion species showed similar or superior leishmanicidal effects to the standart drug N-methylglucamine antimoniate. At the highest concentration of 100 \u00b5g\/mL, cultures of L. guyanensis treated with the venom of B. amazonicus showed the highest mortality percentages, above 28%, while T. metuendus venom showed the highest activity against L. amazonensis, with mortality above 7%. This preliminar study demonstrates that B. amazonicus and T. metuendus venoms can be important tools in the search for new drugs Against leishmaniasis. Next step involves evaluating the activity against the amastigote forms and purifying the venom proteins in order to identify the best anti-leishmania candidates.<\/jats:p>","DOI":"10.1590\/1519-6984.276872","type":"journal-article","created":{"date-parts":[[2023,12,4]],"date-time":"2023-12-04T11:09:50Z","timestamp":1701688190000},"source":"Crossref","is-referenced-by-count":3,"title":["Leishmanicidal activity of the venoms of the Scorpions Brotheas amazonicus and Tityus metuendus"],"prefix":"10.1590","volume":"83","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9378-7153","authenticated-orcid":false,"given":"D. B.","family":"Pereira","sequence":"first","affiliation":[{"name":"Universidade do Estado do Amazonas,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1018-2066","authenticated-orcid":false,"given":"J. G.","family":"Martins","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Pesquisas da Amaz\u00f4nia,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1822-6991","authenticated-orcid":false,"given":"M. S.","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Universidade do Estado do Amazonas,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8237-3307","authenticated-orcid":false,"given":"R. S.","family":"Lima-J\u00fanior","sequence":"additional","affiliation":[{"name":"Universidade do Estado do Amazonas,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-8621-0524","authenticated-orcid":false,"given":"L. C.","family":"Rocha","sequence":"additional","affiliation":[{"name":"Universidade do Estado do Amazonas,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6174-2698","authenticated-orcid":false,"given":"S. L.","family":"Andrade","sequence":"additional","affiliation":[{"name":"Universidade do Estado do Amazonas,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8538-4642","authenticated-orcid":false,"given":"R. E. L.","family":"Proc\u00f3pio","sequence":"additional","affiliation":[{"name":"Universidade do Estado do Amazonas,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","published-online":{"date-parts":[[2023]]},"reference":[{"issue":"4","key":"ref1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.21767\/1791-809X.1000519","article-title":"Leishmaniasis: a review on parasite, vector and reservoir host","volume":"11","author":"ALEMAYEHU B.","year":"2017","journal-title":"Health Science Journal"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/j.peptides.2013.10.021","article-title":"Scorpion venom peptides with no disulfide bridges: a review","volume":"51","author":"ALMAAYTAH A.","year":"2014","journal-title":"Peptides"},{"key":"ref3","series-title":"In vitro evaluation of the leishmanicida activity of fungi isolated from soil samples from the Amazon region.","author":"ALVES T.D.A.","year":"2019"},{"issue":"3","key":"ref4","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1590\/1806-9282.64.03.281","article-title":"Human leishmaniasis in Brazil: a general review","volume":"64","author":"ANVERSA L.","year":"2018","journal-title":"Revista da Associa\u00e7\u00e3o M\u00e9dica Brasileira"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/j.toxicon.2018.01.006","article-title":"Venom characterization of the Amazonian scorpion Tityus metuendus.","volume":"143","author":"BATISTA C.V.F.","year":"2018","journal-title":"Toxicon"},{"key":"ref6","series-title":"Escorpi\u00f5es do Brasil: lista dos g\u00eaneros e esp\u00e9cies de escorpi\u00f5es registrados para o Brasil (Arachnida, Scorpiones). 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with hydrogen peroxide generated by its L-amino acid oxidase","volume":"280","author":"TEMPONE A.G.","year":"2001","journal-title":"Biochemical and Biophysical Research Communications"},{"issue":"4","key":"ref34","doi-asserted-by":"crossref","first-page":"635","DOI":"10.5578\/mb.20219714","article-title":"Kara Akrep (Androctonus crassicauda) venomunun Leishmania tropica promastigotlar\u0131 \u00fczerindeki in vitro etkinli\u011fi","volume":"55","author":"YILDIZ ZEYREK F.","year":"2021","journal-title":"Mikrobiyoloji Bulteni"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"1112","DOI":"10.1016\/j.msec.2015.09.082","article-title":"Study of biomechanical, anatomical, and physiological properties of scorpion stingers for developing biomimetic materials","volume":"58","author":"ZHAO Z.L.","year":"2016","journal-title":"Materials Science and Engineering C"}],"container-title":["Brazilian Journal of Biology"],"original-title":["Atividade leishmanicida dos venenos dos escorpi\u00f5es Brotheas amazonicus e Tityus metuendus"],"link":[{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_pdf&pid=S1519-69842023000101040&tlng=en","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,12,4]],"date-time":"2023-12-04T11:10:04Z","timestamp":1701688204000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_arttext&pid=S1519-69842023000101040&tlng=en"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023]]},"references-count":35,"alternative-id":["S1519-69842023000101040"],"URL":"https:\/\/doi.org\/10.1590\/1519-6984.276872","relation":{},"ISSN":["1678-4375","1519-6984"],"issn-type":[{"value":"1678-4375","type":"electronic"},{"value":"1519-6984","type":"print"}],"subject":[],"published":{"date-parts":[[2023]]},"article-number":"e276872"}}