{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T15:04:46Z","timestamp":1753887886282,"version":"3.41.2"},"reference-count":51,"publisher":"FapUNIFESP (SciELO)","license":[{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Braz. J. Biol."],"abstract":"<jats:p>Abstract Round cell tumors are common cutaneous lesions in dogs, with increased occurrence percentages among different skin tumors. This study aimed to investigate the frequency as well as gross and pathological characteristics of round cell tumors in natural cases of tumorous dogs in relation to breed, sex, and age. Moreover, it aimed to evaluate the immunohistochemical expression of a panel of immunohistochemical stains, including vimentin, E-cadherin, and cluster of differentiation (CD45) as an adjunct technique for the differential diagnosis of cutaneous round cell neoplasm. Data were collected from 64 dogs of both sexes (36 females and 28 males), various breeds, and different ages (8 months to 7 years). The histopathological nature of neoplastic growth was reported, and neoplasm prevalence was classified using age, sex, breed, and site on the body. We observed 48 cases of transmissible venereal tumors, 12 cutaneous histiocytomas, and 4 histiocytic sarcoma. Immunohistochemical characterization revealed an intense positive immunoreactivity for vimentin in transmissible venereal tumor cells and moderate positive immunoreactivity for E-cadherin and CD45 in cutaneous histiocytoma and histiocytic sarcoma cells. In conclusion, the canine transmissible venereal tumor was the most frequent form of round cell tumor; thus, a definitive cutaneous neoplasm diagnosis should be based on histopathological morphology and immunohistochemical findings.<\/jats:p>","DOI":"10.1590\/1519-6984.278769","type":"journal-article","created":{"date-parts":[[2024,2,26]],"date-time":"2024-02-26T11:09:39Z","timestamp":1708945779000},"source":"Crossref","is-referenced-by-count":0,"title":["Immunohistochemical expression of vimentin, E-cadherin, and CD45 in natural cases of canine cutaneous round tumors"],"prefix":"10.1590","volume":"84","author":[{"given":"S. S.","family":"Oda","sequence":"first","affiliation":[{"name":"Alexandria University,  Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. F.","family":"Tawfik","sequence":"additional","affiliation":[{"name":"Alexandria University,  Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5802-6359","authenticated-orcid":false,"given":"S. I.","family":"Othman","sequence":"additional","affiliation":[{"name":"Princess Nourah bint Abdulrahman University,  Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4464-5989","authenticated-orcid":false,"given":"H. A.","family":"Rudayni","sequence":"additional","affiliation":[{"name":"Imam Muhammad Ibn Saud Islamic University,  Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0702-8025","authenticated-orcid":false,"given":"A. A.","family":"Allam","sequence":"additional","affiliation":[{"name":"Imam Muhammad Ibn Saud Islamic University,  Saudi Arabia; Beni-suef University,  Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4235-669X","authenticated-orcid":false,"given":"A. F.","family":"Khafaga","sequence":"additional","affiliation":[{"name":"Alexandria University,  Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","published-online":{"date-parts":[[2024]]},"reference":[{"issue":"1","key":"ref1","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1354\/vp.39-1-74","article-title":"Localized and disseminated histiocytic sarcoma of dendritic cell origin in dogs","volume":"39","author":"AFFOLTER V.K.","year":"2002","journal-title":"Veterinary Pathology"},{"first-page":"1","year":"2004","author":"AFFOLTER V.K.","key":"ref2"},{"key":"ref3","article-title":"Intra-abdominal transmissible venereal tumor in a dog: a case report","volume":"44","author":"ALEXANDRE J.B.","year":"2022","journal-title":"Brazilian Journal of Veterinary Medicine"},{"key":"ref4","series-title":"Small animal dermatology: a color atlas and therapeutic guide","first-page":"448","volume-title":"Neoplastic and nonneoplastic tumors.","author":"AMY L.","year":"2017","edition":"4th"},{"issue":"8","key":"ref5","doi-asserted-by":"crossref","first-page":"772","DOI":"10.1590\/S0100-736X2012000800016","article-title":"Histomorphological and immunohistochemical characterization of 172 cutaneous round cell tumours in dogs","volume":"32","author":"ARAUJO M.R.","year":"2012","journal-title":"Pesquisa Veterin\u00e1ria Brasileira"},{"issue":"16","key":"ref6","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1136\/vr.162.16.509","article-title":"E-cadherin expression in canine cutaneous histiocytomas","volume":"162","author":"BAINES S.J.","year":"2008","journal-title":"The Veterinary Record"},{"key":"ref7","series-title":"Theory and practice of histological techniques.","first-page":"252","author":"BANCROFT J.D.","year":"2013","edition":"7th"},{"issue":"1","key":"ref8","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1186\/1751-0147-52-6","article-title":"Mast cell tumours and other skin neoplasia in Danish dogs--data from the Danish veterinary cancer registry","volume":"52","author":"BR\u00d8NDEN L.B.","year":"2010","journal-title":"Acta Veterinaria Scandinavica"},{"issue":"8","key":"ref9","doi-asserted-by":"crossref","first-page":"958","DOI":"10.5858\/arpa.2018-0112-RA","article-title":"Cutaneous spindle cell neoplasms pattern-based diagnostic approach","volume":"142","author":"CHOI J.H.","year":"2018","journal-title":"Archives of Pathology & Laboratory Medicine"},{"key":"ref10","first-page":"64","article-title":"Epidemiological aspects and differential diagnosis of the cutaneous round cell tumors in dogs","volume":"74","author":"CORA R.","year":"2017","journal-title":"Bulletin UASVM Veterinary Medicine"},{"issue":"5","key":"ref11","doi-asserted-by":"crossref","first-page":"769","DOI":"10.1177\/10406387221100209","article-title":"Review of diagnostic histologic features of cutaneous round cell neoplasms in dogs","volume":"34","author":"DANIEL R.R.","year":"2022","journal-title":"Journal of Veterinary Diagnostic Investigation"},{"key":"ref12","first-page":"1","article-title":"Temporospatial distribution and country of origin of canine transmissible venereal tumours in the UK","volume":"974","author":"DANIELLE N.G.","year":"2021","journal-title":"The Veterinary Record"},{"key":"ref13","series-title":"Withrow and MacEwen\u2019s small animal clinical oncology.","first-page":"799","volume-title":"Miscellaneous tumors.","author":"DELORIMIER L.P.","year":"2020","edition":"6th"},{"key":"ref14","series-title":"Laboratory Animal Medicine","first-page":"395","volume-title":"Biology and diseases of dogs.","author":"DYSKO R.C.","year":"2002"},{"key":"ref15","series-title":"Jubb, Kennedy & Palmer\u2019s Pathology of Domestic Animals","first-page":"611","volume-title":"Skin and appendages.","author":"ELIZABETH A.","year":"2016","edition":"6th"},{"issue":"1","key":"ref16","first-page":"7","article-title":"Morphological distinction of histiocytic sarcoma from other tumor types in bernese mountain dogs and flat coated retrievers","volume":"32","author":"ERICH S.A.","year":"2018","journal-title":"In Vivo (Athens, Greece)"},{"issue":"2","key":"ref17","doi-asserted-by":"crossref","first-page":"204","DOI":"10.29261\/pakvetj\/2018.044","article-title":"Clinico-pathological evaluation and treatment outcomes of canine transmissible venereal tumor using three different protocols","volume":"38","author":"FATHI M.","year":"2018","journal-title":"Pakistan Veterinary Journal"},{"issue":"4","key":"ref18","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1354\/vp.42-4-437","article-title":"Immunohistochemical and Histochemical stains for differentiating canine cutaneous round cell tumors","volume":"42","author":"FERNANDEZ N.J.","year":"2005","journal-title":"Veterinary Pathology"},{"issue":"2","key":"ref19","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1111\/j.1476-5810.2006.00090.x","article-title":"Histiocytic sarcomas in flat-coated retrievers: a summary of 37 cases (November 1998-March 2005)","volume":"4","author":"FIDEL J.","year":"2006","journal-title":"Veterinary and Comparative Oncology"},{"issue":"10","key":"ref20","first-page":"1041","article-title":"Canine histiocytic neoplasia: an overview","volume":"48","author":"FULMER A.K.","year":"2007","journal-title":"The Canadian Veterinary Journal. La Revue Veterinaire Canadienne"},{"issue":"4","key":"ref21","doi-asserted-by":"crossref","first-page":"412","DOI":"10.1354\/vp.40-4-412","article-title":"p63: a novel myoepithelial cell marker in canine mammary tissues","volume":"40","author":"GAMA A.","year":"2003","journal-title":"Veterinary Pathology"},{"key":"ref22","series-title":"Tumors in domestic animals.","first-page":"88","volume-title":"Epithelial and melanocytic tumors of the skin.","author":"GOLDSCHMIDT M.H.","year":"2020","edition":"5th"},{"key":"ref23","series-title":"Skin diseases of the dog and cat: clinical and histopathologic diagnosis","first-page":"858","volume-title":"Histiocytic tumors","author":"GROSS T.L.","year":"2009","edition":"2nd"},{"key":"ref24","series-title":"Tumors in domestic animals.","first-page":"142","volume-title":"Mesenchymal tumors of the skin and soft tissues.","author":"HENDRICK M.J.","year":"2020","edition":"5th"},{"issue":"1","key":"ref25","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1111\/j.1476-5829.2009.00204.x","article-title":"Risk factors and characteristics of canine transmissible venereal tumours in Grenada, West Indies","volume":"8","author":"KABUUSU R.M.","year":"2010","journal-title":"Veterinary and Comparative Oncology"},{"issue":"1","key":"ref26","doi-asserted-by":"crossref","first-page":"129","DOI":"10.33899\/ijvs.2019.125624.1111","article-title":"Incidence 300 of cutaneous and subcutaneous tumors of dogs from Baghdad city: clinical, cytological and histopathological features","volume":"34","author":"LAFTA I.J.","year":"2020","journal-title":"Iraqi Journal of Veterinary Sciences"},{"issue":"1-2","key":"ref27","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0165-2427(96)05757-1","article-title":"Immunophenotype of the canine transmissible venereal tumor","volume":"57","author":"MARCHAL T.","year":"1997","journal-title":"Veterinary Immunology and Immunopathology"},{"issue":"3","key":"ref28","doi-asserted-by":"crossref","first-page":"250","DOI":"10.1590\/S0100-736X2014000300009","article-title":"Immunohistochemical study of genital and extragenital forms of canine transmissible venereal tumor in Brazil","volume":"34","author":"MASCARENHAS M.B.","year":"2014","journal-title":"Pesquisa Veterin\u00e1ria Brasileira"},{"issue":"3","key":"ref29","doi-asserted-by":"crossref","first-page":"412","DOI":"10.1111\/j.1939-165X.2012.00449.x","article-title":"Multifocal cutaneous histiocytic sarcoma in a young dog and review of histiocytic cell immunophenotyping","volume":"41","author":"MASTRORILLI C.","year":"2012","journal-title":"Veterinary Clinical Pathology"},{"key":"ref30","series-title":"Schalm\u2019s Veterinary Hematology.","first-page":"540","volume-title":"Histiocytic proliferative diseases.","author":"MOORE P.F.","year":"2010","edition":"6th"},{"first-page":"437","year":"2004","author":"MOORE P.F.","key":"ref31"},{"key":"ref32","series-title":"Tumors in domestic animals.","first-page":"322","volume-title":"Canine and feline histiocytic diseases.","author":"MOORE P.F.","year":"2020","edition":"5th"},{"issue":"5","key":"ref33","first-page":"1699","article-title":"Canine cutaneous histiocytoma is an epidermotropic Langerhans cell histiocytosis that expresses CD1 and specific f32-integrin molecules","volume":"148","author":"MOORE P.F.","year":"1996","journal-title":"American Journal of Pathology"},{"issue":"3","key":"ref34","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1177\/030098589603300301","article-title":"Immunohistochemical characterization of canine transmissible venereal tumor","volume":"33","author":"MOZOS E.","year":"1996","journal-title":"Veterinary Pathology"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"1237","DOI":"10.21608\/ejvs.2023.219337.1529","article-title":"Comparative study between IV and IM Co-administration of Xylazine with ketamine on some physiological parameters in dogs","volume":"54","author":"NIWAR H.H.","year":"2023","journal-title":"Egyptian Journal of Veterinary Science"},{"issue":"1","key":"ref36","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.tig.2015.10.001","article-title":"Transmissible tumors: breaking the cancer paradigm","volume":"32","author":"OSTRANDER E.A.","year":"2016","journal-title":"Trends in Genetics"},{"issue":"7","key":"ref37","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1046\/j.1439-0442.2001.00361.x","article-title":"Primary intranasal transmissible venereal tumor in the dog: a retrospective study of six cases","volume":"48","author":"PAPAZOGLOU L.G.","year":"2001","journal-title":"Journal of Veterinary Medicine. A, Physiology, Pathology, Clinical Medicine"},{"issue":"1","key":"ref38","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1177\/104063870601800123","article-title":"Disseminated transmissible venereal tumor in a dog","volume":"18","author":"PARK M.S.","year":"2006","journal-title":"Journal of Veterinary Diagnostic Investigation"},{"issue":"1","key":"ref39","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1007\/s11259-014-9622-1","article-title":"Morphology and immunophenotype of canine cutaneous histiocytic tumours with particular emphasis on diagnostic application","volume":"39","author":"PA\u0179DZIOR-CZAPULA K.","year":"2015","journal-title":"Veterinary Research Communications"},{"issue":"1","key":"ref40","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1046\/j.1463-5224.2000.00097.x","article-title":"Immunohistochemical characterization of intraocular metastasis of a canine transmissible venereal tumor","volume":"3","author":"PEREIRA J.S.","year":"2000","journal-title":"Veterinary Ophthalmology"},{"issue":"3","key":"ref41","doi-asserted-by":"crossref","first-page":"758","DOI":"10.1177\/0300985811398251","article-title":"Immunohistochemical expression of E-cadherin does not distinguish canine cutaneous histiocytoma from other canine round cell tumors","volume":"48","author":"RAMOS-VARA J.A.","year":"2011","journal-title":"Veterinary Pathology"},{"issue":"4","key":"ref42","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1177\/104063870802000401","article-title":"Suggested guidelines for immunohistochemical techniques in veterinary diagnostic laboratories","volume":"20","author":"RAMOS-VARA J.A.","year":"2008","journal-title":"Journal of Veterinary Diagnostic Investigation"},{"year":"2004","series-title":"Statistical analysis system.","key":"ref43"},{"issue":"2","key":"ref44","first-page":"172","article-title":"Benign cutaneous histocytomas in two dogs","volume":"6","author":"SAWALE G.K.","year":"2014","journal-title":"Indian Journal of Canine Practice"},{"key":"ref45","series-title":"Jubb, Kennedy & Palmer\u2019s Pathology of Domestic Animals","first-page":"429","volume-title":"Female genital system.","author":"SCHLAFER D.H.","year":"2016","edition":"6th"},{"issue":"1","key":"ref46","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1186\/1756-9966-27-58","article-title":"Biphasic pulses enhance bleomycin efficacy in a spontaneous canine genital tumor model of chemoresistance: sticker sarcoma","volume":"27","author":"SPUGNINI E.P.","year":"2008","journal-title":"Journal of Experimental & Clinical Cancer Research"},{"issue":"7","key":"ref47","doi-asserted-by":"crossref","first-page":"1167","DOI":"10.1292\/jvms.15-0710","article-title":"Cell-based polymerase chain reaction for canine transmissible venereal tumor (CTVT) diagnosis","volume":"78","author":"STTHAWONGSIN C.","year":"2016","journal-title":"The Journal of Veterinary Medical Science"},{"key":"ref48","first-page":"71","article-title":"Retrospective study of canine cutaneous neoplasia","volume":"22","author":"TOSTES R.A.","year":"2017","journal-title":"Archives of Veterinary Science"},{"key":"ref49","series-title":"Withrow and MacEwen\u2019s Small Animal Clinical Oncology.","first-page":"352","volume-title":"Tumors of the Skin and Subcutaneous Tissues.","author":"VAIL D.M.","year":"2020","edition":"6th"},{"key":"ref50","series-title":"Jubb, Kennedy & Palmer\u2019s Pathology of Domestic Animals","first-page":"109","volume-title":"Hematopoietic system.","author":"VALLI V.E.","year":"2016","edition":"6th"},{"issue":"5","key":"ref51","doi-asserted-by":"crossref","first-page":"620","DOI":"10.4142\/jvs.2018.19.5.620","article-title":"Detection of progressive and regressive phase and LINE-1 retrotransposon in transfected dogs with transmissible venereal tumor during chemotherapy","volume":"19","author":"VURAL S.A.","year":"2018","journal-title":"Journal of Veterinary Science (Suwon-si, Korea)"}],"container-title":["Brazilian Journal of Biology"],"original-title":["Express\u00e3o imuno-histoqu\u00edmica de vimentina, E-caderina e CD45 em casos naturais de tumores redondos cut\u00e2neos em c\u00e3es"],"link":[{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_pdf&pid=S1519-69842024000100486&tlng=en","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,2,26]],"date-time":"2024-02-26T11:10:03Z","timestamp":1708945803000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_arttext&pid=S1519-69842024000100486&tlng=en"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024]]},"references-count":51,"alternative-id":["S1519-69842024000100486"],"URL":"https:\/\/doi.org\/10.1590\/1519-6984.278769","relation":{},"ISSN":["1678-4375","1519-6984"],"issn-type":[{"type":"electronic","value":"1678-4375"},{"type":"print","value":"1519-6984"}],"subject":[],"published":{"date-parts":[[2024]]},"article-number":"e278769"}}