{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,16]],"date-time":"2025-12-16T12:49:17Z","timestamp":1765889357247,"version":"3.41.2"},"reference-count":72,"publisher":"FapUNIFESP (SciELO)","license":[{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Braz. J. Biol."],"abstract":"<jats:p>Abstract Producers of fish have been looking for viable alternatives for the management of Colossoma macropomum (tambaqui) in confinement systems in order to avoid the harm and subsequent losses caused by parasitic diseases. One alternative used by farmers is pesticides, such as trichlorfon, which has a genotoxic effect. Thus, this study aimed to evaluate the changes in gene expression due to the side effects of trichlorfon in tambaqui. Two treatments were used based on LC50-96h of 0.870 mg\/L using 30% and 50% trichlorfon with exposure periods of 48, 72 and 96 h. For differential expression of the genes in the liver, real-time PCR was performed for the AChE, GST, CYP2J6, CYP2C8, 18S and GAPDH genes. After 96 h of exposure to trichlorfon, an alteration in the gene expression profile of the antioxidant defense system (GST) of the tambaqui was observed. It was also observed that this organophosphate did not affect the expression of genes related to the isoenzymes that are responsible for the biotransformation of xenobiotics in phase I (2J6 and 2C8) and cholinesterase AChE. It was concluded that the reduction in gene expression of GST suggests a decrease in metabolization capacity in phase II.<\/jats:p>","DOI":"10.1590\/1519-6984.281971","type":"journal-article","created":{"date-parts":[[2024,7,9]],"date-time":"2024-07-09T03:54:45Z","timestamp":1720497285000},"source":"Crossref","is-referenced-by-count":2,"title":["Effects of trichlorfon on ecotoxicological biomarkers in farmed Colossoma macropomum (tambaqui)"],"prefix":"10.1590","volume":"84","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9064-0447","authenticated-orcid":false,"given":"A. P. C.","family":"Carvalho","sequence":"first","affiliation":[{"name":"Instituto Nacional de Pesquisas da Amaz\u00f4nia,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8998-0855","authenticated-orcid":false,"given":"H. C. M.","family":"Silva","sequence":"additional","affiliation":[{"name":"Universidade Estadual de Ponta Grossa,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3916-6863","authenticated-orcid":false,"given":"A. L. S.","family":"Gomes","sequence":"additional","affiliation":[{"name":"Universidade Federal do Amazonas,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1279-7163","authenticated-orcid":false,"given":"W. L. P.","family":"Duncan","sequence":"additional","affiliation":[{"name":"Universidade Federal do Amazonas,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9745-1128","authenticated-orcid":false,"given":"A. J.","family":"Mota","sequence":"additional","affiliation":[{"name":"Universidade Federal do Amazonas,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2528-9846","authenticated-orcid":false,"given":"R. F.","family":"Artoni","sequence":"additional","affiliation":[{"name":"Universidade Estadual de Ponta Grossa,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1933-8899","authenticated-orcid":false,"given":"G.","family":"Carvalho-Zilse","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Pesquisas da Amaz\u00f4nia,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7346-6352","authenticated-orcid":false,"given":"D. A.","family":"Matoso","sequence":"additional","affiliation":[{"name":"Universidade Federal do Amazonas,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","published-online":{"date-parts":[[2024]]},"reference":[{"issue":"8","key":"ref1","doi-asserted-by":"crossref","first-page":"1028","DOI":"10.1016\/j.chemosphere.2012.04.001","article-title":"Histopathological biomarkers in gills and liver of Oreochromis niloticus from polluted wetland environments, Saudi Arabia","volume":"88","author":"ABDEL-MONEIM A.M.","year":"2012","journal-title":"Chemosphere"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/j.jobaz.2015.11.002","article-title":"Responses of metabolic and antioxidant enzymatic activities in gill, liver and plasma of Catla catla during methyl parathion exposure","volume":"77","author":"ABHIJITH B.D.","year":"2016","journal-title":"Journal of Basic & Applied Zoology"},{"issue":"10","key":"ref3","doi-asserted-by":"crossref","first-page":"2243","DOI":"10.1002\/etc.272","article-title":"Characterization of acetylcholinesterase from the brain of the amazonian tambaqui (Colossoma macropomum) and in vitro effect of organophosphorus and carbamate pesticides","volume":"29","author":"ASSIS C.R.D.","year":"2010","journal-title":"Environmental Toxicology and Chemistry"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1016\/j.cbi.2018.11.015","article-title":"Disturbance of energetic homeostasis and oxidative damage provoked by trichlorfon as relevant toxicological mechanisms using silver catfish as experimental model","volume":"299","author":"BALDISSERA M.D.","year":"2019","journal-title":"Chemico-Biological Interactions"},{"issue":"3","key":"ref5","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1016\/j.pestbp.2010.09.002","article-title":"Effects of the organophosphate pesticide folidol 600\u00ae on the freshwater fish, nile tilapia (Oreochromis niloticus)","volume":"99","author":"BARBIERI E.","year":"2011","journal-title":"Pesticide Biochemistry and Physiology"},{"issue":"7","key":"ref6","doi-asserted-by":"crossref","first-page":"287","DOI":"10.22256\/pubvet.v9n7.287-290","article-title":"Caracter\u00edsticas zoot\u00e9cnicas e potenciais do tambaqui (Colossoma macropomum) para a piscicultura brasileira","volume":"9","author":"BAR\u00c7ANTE B.","year":"2015","journal-title":"Pubvet"},{"issue":"249","key":"ref7","doi-asserted-by":"crossref","first-page":"21","DOI":"10.21071\/az.v65i249.437","article-title":"Custo de implanta\u00e7\u00e3o e planejamento de uma piscicultura de grande porte no Estado de Mato Grosso, Brasil","volume":"65","author":"BARROS A.F.","year":"2016","journal-title":"Archivos de Zootecnia"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1016\/j.ecolind.2016.02.011","article-title":"Alterations in the general condition, biochemical parameters and locomotor activity in Cnesterodon decemmaculatus exposed to commercial formulations of chlorpyrifos, glyphosate and their mixtures","volume":"67","author":"BONIFACIO A.F.","year":"2016","journal-title":"Ecological Indicators"},{"year":"2003","series-title":"Potencialidades - 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