{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T03:17:56Z","timestamp":1787023076627,"version":"build-2736575974"},"reference-count":20,"publisher":"FapUNIFESP (SciELO)","license":[{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Braz. J. Biol."],"abstract":"<jats:p>Abstract At present, non-alcoholic fatty liver disease (NAFLD) is the most prevalent liver disease worldwide, with obesity recognized as a global epidemic and type 2 diabetes a worldwide disease. In this study, 10 C57BL\/6 mice were divided into two groups: the control group (SC) and the high-fat diet (HF) group. Both groups were fed their respective diets for 8 weeks. The animals were analyzed for body weight, glucose\/insulin resistance, hepatic steatosis, and fibrosis to diagnose NAFLD. Results showed that the HF group animals had significantly higher body weight (P&lt;0.0001), glucose resistance (P=0.0002), insulin resistance (P=0.0009), and blood glucose levels (P&lt;0.05) compared to the SC group. The HF group exhibited increased hepatic steatosis (P&lt;0.0001) and fibrosis (P&lt;0.0001) compared to the SC group. These findings led to the conclusion that the animals in the HF group had grade and stage 2 NAFLD. Furthermore, the HF group animals were classified as obese, indicating a higher risk for developing insulin resistance and, subsequently, type 2 diabetes mellitus (T2DM). Understanding the risk factors and complications associated with NAFLD, obesity, and T2DM is crucial for preventing and treating metabolic alterations linked to a high-fat diet.<\/jats:p>","DOI":"10.1590\/1519-6984.284045","type":"journal-article","created":{"date-parts":[[2025,1,31]],"date-time":"2025-01-31T05:46:44Z","timestamp":1738302404000},"source":"Crossref","is-referenced-by-count":1,"title":["Liver under attack: impacts of high-fat diet on murine model"],"prefix":"10.1590","volume":"84","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1848-3878","authenticated-orcid":false,"given":"P. F.","family":"Peppi","sequence":"first","affiliation":[{"name":"Universidade Federal do Paran\u00e1,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-5334-3194","authenticated-orcid":false,"given":"G. A.","family":"Lira","sequence":"additional","affiliation":[{"name":"Universidade de Bras\u00edlia,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-7722-4316","authenticated-orcid":false,"given":"L. R. S.","family":"Campos","sequence":"additional","affiliation":[{"name":"Universidade de Bras\u00edlia,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-5892-1355","authenticated-orcid":false,"given":"C. R.","family":"Santos","sequence":"additional","affiliation":[{"name":"Universidade de Bras\u00edlia,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7449-8428","authenticated-orcid":false,"given":"E. M. M.","family":"Lima","sequence":"additional","affiliation":[{"name":"Universidade de Bras\u00edlia,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8354-3163","authenticated-orcid":false,"given":"A. R. C.","family":"Barreto-Vianna","sequence":"additional","affiliation":[{"name":"Universidade Federal do Paran\u00e1,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"530","published-online":{"date-parts":[[2024]]},"reference":[{"issue":"16","key":"ref1","doi-asserted-by":"crossref","first-page":"715","DOI":"10.4254\/wjh.v9.i16.715","article-title":"Non-alcoholic fatty liver disease: an expanded review","volume":"9","author":"BENEDICT M.","year":"2017","journal-title":"World Journal of Hepatology"},{"issue":"5","key":"ref2","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1007\/s00428-011-1147-1","article-title":"A critical analysis of three quantitative methods of assessment of hepatic steatosis in liver biopsies","volume":"459","author":"CATTA-PRETA M.","year":"2011","journal-title":"Virchows Archiv"},{"issue":"1","key":"ref3","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1111\/hepr.12333","article-title":"Diagnosis and classification of non\u2010alcoholic fatty liver disease and non\u2010alcoholic steatohepatitis: current concepts and remaining challenges","volume":"45","author":"HASHIMOTO E.","year":"2015","journal-title":"Hepatology Research"},{"issue":"3","key":"ref4","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1097\/MOG.0b013e3283279668","article-title":"Hepatic fibrosis","volume":"25","author":"JIAO J.","year":"2009","journal-title":"Current Opinion in Gastroenterology"},{"key":"ref5","article-title":"Comparative effects of thymol and vitamin E on nonalcoholic fatty liver disease in male Wistar rats","volume":"84","author":"LAHMI A.","year":"2023","journal-title":"Brazilian Journal of Biology = Revista Brasileira de Biologia"},{"issue":"1","key":"ref6","doi-asserted-by":"crossref","first-page":"19556","DOI":"10.1038\/s41598-019-55987-x","article-title":"Effects of different diets used in diet-induced obesity models on insulin resistance and vascular dysfunction in C57BL\/6 mice","volume":"9","author":"LANG P.","year":"2019","journal-title":"Scientific Reports"},{"issue":"8","key":"ref7","doi-asserted-by":"crossref","first-page":"755","DOI":"10.1016\/S2468-1253(22)00061-9","article-title":"Quality standards for the management of Non-Alcoholic Fatty Liver Disease (NAFLD): consensus recommendations from the British Association for the Study of the Liver and British Society of Gastroenterology NAFLD Special Interest Group","volume":"7","author":"MCPHERSON S.","year":"2022","journal-title":"The Lancet. 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