{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T15:09:01Z","timestamp":1753888141305,"version":"3.41.2"},"reference-count":31,"publisher":"FapUNIFESP (SciELO)","license":[{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Braz. J. Biol."],"abstract":"<jats:p>Abstract One of the biggest public health problems globally is that of iron deficiency anemia. The present research aimed to determine the effect of prebiotics along with iron fortification on iron biomarkers in female anemic rats as some evidence suggests that prebiotics convert increase the solubility of iron, thereby enhancing its absorption. A total of 126 Sprague Dawley rats were fed with sixteen different types of fortified feed containing prebiotics (Inulin + Galacto Oligosaccharides) and Iron Fortificants (Sodium Ferric Ethylenediaminetetraacetate + Ferrous Sulphate). The duration of the trials was 3 months aimed at determining the effect of iron fortification and prebiotics on different iron biomarkers including Hemoglobin (Hb), Hematocrit, Red Blood Cell (RBC) count, Mean Corpuscular Volume (MCV), Mean Corpuscular Hemoglobin (MCH) and Mean Corpuscular Hemoglobin Concentration (MCHC). The trials resulted in statistically significant improved iron biomarkers among female anemic rats (P-value &lt; 0.05). It was concluded that iron fortification and prebiotics in combination were able to increase the levels of iron biomarkers in female anemic rats.<\/jats:p>","DOI":"10.1590\/1519-6984.284867","type":"journal-article","created":{"date-parts":[[2025,1,27]],"date-time":"2025-01-27T13:40:50Z","timestamp":1737985250000},"source":"Crossref","is-referenced-by-count":1,"title":["Effect of iron fortification and prebiotics on iron biomarkers in anemic rats"],"prefix":"10.1590","volume":"84","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2552-3927","authenticated-orcid":false,"given":"S.","family":"Iqbal","sequence":"first","affiliation":[{"name":"Al-Ain University,  United Arab Emirates"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-1850-5081","authenticated-orcid":false,"given":"S.","family":"Zafar","sequence":"additional","affiliation":[{"name":"Health Services Academy,  Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2082-4301","authenticated-orcid":false,"given":"W.","family":"Ahmed","sequence":"additional","affiliation":[{"name":"University of Veterinary & Animal Sciences,  Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4205-1140","authenticated-orcid":false,"given":"S. H. B. U.","family":"Shah","sequence":"additional","affiliation":[{"name":"University of New South Wales,  Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3901-1607","authenticated-orcid":false,"given":"J.","family":"Abid","sequence":"additional","affiliation":[{"name":"National University of Medical Sciences,  Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6421-9197","authenticated-orcid":false,"given":"U.","family":"Farooq","sequence":"additional","affiliation":[{"name":"National University of Medical Sciences,  Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3499-902X","authenticated-orcid":false,"given":"A. M. R.","family":"Ahmad","sequence":"additional","affiliation":[{"name":"National University of Medical Sciences,  Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","published-online":{"date-parts":[[2024]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","DOI":"10.1155\/2014\/561567","article-title":"Prevalence of anemia and associated factors among pregnant women in an urban area of Eastern Ethiopia","volume":"2014","author":"ADDIS ALENE K.","year":"2014","journal-title":"Anemia"},{"issue":"3","key":"ref2","first-page":"340","article-title":"Iron status of the Pakistani population-current issues and strategies","volume":"22","author":"AKHTAR S.","year":"2013","journal-title":"Asia Pacific Journal of Clinical Nutrition"},{"issue":"2","key":"ref3","doi-asserted-by":"crossref","first-page":"73","DOI":"10.2174\/1996327001104020073","article-title":"Effect of dehydration in the presence and absence of the angiotensin receptor blocker losartan on blood constituents in the camel","volume":"4","author":"AL HAJ M.","year":"2011","journal-title":"Journal of Medical Sciences"},{"issue":"32","key":"ref4","doi-asserted-by":"crossref","first-page":"8815","DOI":"10.1080\/10408398.2021.1935442","article-title":"Prevalence and approaches to manage iron deficiency anemia (IDA)","volume":"62","author":"BATHLA S.","year":"2022","journal-title":"Critical Reviews in Food Science and Nutrition"},{"issue":"8","key":"ref5","doi-asserted-by":"crossref","first-page":"2239","DOI":"10.3390\/nu12082239","article-title":"Supplemental microalgal iron helps replete blood hemoglobin in moderately anemic mice fed a rice-based diet","volume":"12","author":"BHATNAGAR R.S.","year":"2020","journal-title":"Nutrients"},{"issue":"1","key":"ref6","doi-asserted-by":"crossref","first-page":"18","DOI":"10.18488\/journal.58.2019.61.18.27","article-title":"A review: role of inulin in animal nutrition","volume":"6","author":"BIRMANI M.W.","year":"2019","journal-title":"Journal of Food Technology Research"},{"issue":"9","key":"ref7","doi-asserted-by":"crossref","first-page":"1008","DOI":"10.1080\/003655202320378176","article-title":"Influence of short-chain fatty acids on iron absorption by proximal colon","volume":"37","author":"BOUGLE D.","year":"2002","journal-title":"Scandinavian Journal of Gastroenterology"},{"issue":"6","key":"ref8","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1038\/sj.ejcn.1600417","article-title":"Effect of soluble or partly soluble dietary fibres supplementation on absorption and balance of calcium, magnesium, iron and zinc in healthy young men","volume":"51","author":"COUDRAY C.","year":"1997","journal-title":"European Journal of Clinical Nutrition"},{"issue":"2","key":"ref9","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1111\/1471-0307.12575","article-title":"Iron fortification of reduced\u2010fat bioyoghurt containing either short\u2010or long\u2010chain inulin","volume":"72","author":"DABOUR N.","year":"2019","journal-title":"International Journal of Dairy Technology"},{"year":"2018","series-title":"Biostatistics: a foundation for analysis in the health sciences.","author":"DANIEL W.W.","key":"ref10"},{"issue":"6","key":"ref11","doi-asserted-by":"crossref","first-page":"2296","DOI":"10.1093\/advances\/nmac079","article-title":"The effect of prebiotics on human iron absorption: a review","volume":"13","author":"HUSMANN F.","year":"2022","journal-title":"Advances in Nutrition"},{"year":"1986","series-title":"Guide for the care and use of laboratory animals","key":"ref12"},{"issue":"2","key":"ref13","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1080\/13685538.2017.1350156","article-title":"Reference database of hematological parameters for growing and aging rats","volume":"21","author":"JACOB FILHO W.","year":"2018","journal-title":"The Aging Male"},{"issue":"5","key":"ref14","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1016\/S2352-4642(20)30274-1","article-title":"Effective interventions to address maternal and child malnutrition: an update of the evidence","volume":"5","author":"KEATS E.C.","year":"2021","journal-title":"The Lancet. 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