{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T11:42:37Z","timestamp":1784893357836,"version":"3.55.0"},"reference-count":47,"publisher":"FapUNIFESP (SciELO)","license":[{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Braz. J. Biol."],"abstract":"<jats:p>Abstract Immunosuppressed individuals, including those undergoing cancer treatment, are more vulnerable to fungal infections, such as oral candidiasis, impacting their quality of life. Given the limitations of current therapies, the discovery of new antifungal agents, including those of natural origin, is crucial for the proper managing of these infections. We investigated the phytochemical profile and antifungal activity of both the essential oil and crude ethanolic extract (CEE) obtained from Eugenia luschnathiana against reference strains and clinical isolates of Candida from oncology patients. Toxicological characterization was also conducted. Gas chromatography coupled to mass spectrometry (GC-MS) and 1H Nuclear Magnetic Resonance (NMR) were used for phytochemical analysis. Antifungal evaluation was conducted to determine the Minimum Inhibitory Concentration (MIC) and Minimum Fungicidal Concentration (MFC); evaluation of potential mechanisms of action; activity on a fungal biofilm; evaluation of the cytotoxic effect on human keratinocytes of the HaCat lineage by the MTT method; determination of lethality for Artemia salina larvae. GC-MS identified a predominance of sesquiterpenes in the essential oil, notably (E)-Caryophyllene. The 1H NMR spectrum identified aliphatic, osidic, and aromatic compounds in the crude ethanolic extract. The essential oil showed no antifungal activity. However, the CEE exhibited fungicidal activity, with MIC and MFC ranging from 1.95 \u00b5g\/mL to 3.90 \u00b5g\/mL. The antifungal effect was affected by sorbitol, indicating a possible mechanism targeting fungal cell wall structures. At low concentration (19.5 \u00b5g\/mL), the CEE inhibited 62,78% of C. albicans biofilm. The CEE demonstrated a promising toxicity profile, with an LC50 of 142.4 \u00b5g\/mL against Artemia salina. In conclusion, the CEE from Eugenia luschnathiana exhibited potent antifungal activity, likely through cell wall disruption, biofilm inhibition, and a favorable toxicity profile for further exploration.<\/jats:p>","DOI":"10.1590\/1519-6984.286419","type":"journal-article","created":{"date-parts":[[2024,9,13]],"date-time":"2024-09-13T13:58:43Z","timestamp":1726235923000},"source":"Crossref","is-referenced-by-count":4,"title":["Bioactive potential of Eugenia luschnathiana essential oil and extract: antifungal activity against Candida species isolated from oncological patients"],"prefix":"10.1590","volume":"84","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3983-9781","authenticated-orcid":false,"given":"P. C. Q. G. da","family":"Costa","sequence":"first","affiliation":[{"name":"Universidade Federal da Para\u00edba,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7425-3201","authenticated-orcid":false,"given":"P. L.","family":"Nogueira","sequence":"additional","affiliation":[{"name":"Universidade Federal da Para\u00edba,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9732-6191","authenticated-orcid":false,"given":"Y. M. do","family":"Nascimento","sequence":"additional","affiliation":[{"name":"Universidade Federal da Para\u00edba,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7740-034X","authenticated-orcid":false,"given":"M. V.","family":"Sobral","sequence":"additional","affiliation":[{"name":"Universidade Federal da Para\u00edba,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5783-0449","authenticated-orcid":false,"given":"G. F. G.","family":"Silvestre","sequence":"additional","affiliation":[{"name":"Universidade Federal da Para\u00edba,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7986-7376","authenticated-orcid":false,"given":"R. D. de","family":"Castro","sequence":"additional","affiliation":[{"name":"Universidade Federal da Para\u00edba,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"530","published-online":{"date-parts":[[2024]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","DOI":"10.1155\/2021\/6653311","article-title":"Breakpoints for the Classification of Anti-Candida Compounds in Antifungal Screening","volume":"2021","author":"ALVES D.N.","year":"2021","journal-title":"BioMed Research International"},{"issue":"24","key":"ref2","doi-asserted-by":"crossref","first-page":"5969","DOI":"10.3390\/molecules25245969","article-title":"Docking prediction, antifungal activity, anti-biofilm effects on Candida spp., and toxicity against human cells of cinnamaldehyde","volume":"25","author":"ALVES D.","year":"2020","journal-title":"Molecules"},{"key":"ref3","series-title":"Investiga\u00e7\u00e3o qu\u00edmica de Eugenia luschnathiana: determina\u00e7\u00e3o da composi\u00e7\u00e3o vol\u00e1til, isolamento e identifica\u00e7\u00e3o de triterpenos oleananos e ursanos","author":"ARA\u00daJO R.D.","year":"2018"},{"issue":"3","key":"ref4","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1007\/s11046-020-00445-w","article-title":"Candida biofilms: an update on developmental mechanisms and therapeutic challenges","volume":"185","author":"BARROS P.P.","year":"2020","journal-title":"Mycopathologia"},{"issue":"1","key":"ref5","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1146\/annurev-micro-032521-015858","article-title":"Candida auris genetics and emergence","volume":"77","author":"CHOWDHARY A.","year":"2023","journal-title":"Annual Review of Microbiology"},{"issue":"5","key":"ref6","doi-asserted-by":"crossref","first-page":"441","DOI":"10.2174\/1567201819666220418104042","article-title":"Oral candidiasis and novel therapeutic strategies: antifungals, phytotherapy, probiotics, and photodynamic therapy","volume":"20","author":"CONTALDO M.","year":"2023","journal-title":"Current Drug Delivery"},{"issue":"2","key":"ref7","doi-asserted-by":"crossref","first-page":"328","DOI":"10.3390\/biom10020328","article-title":"Seasonal and antioxidant evaluation of essential oil from Eugenia uniflora L., curzerene-rich, thermally produced in situ","volume":"10","author":"COSTA J.S.","year":"2020","journal-title":"Biomolecules"},{"issue":"6","key":"ref8","doi-asserted-by":"crossref","first-page":"2950","DOI":"10.1128\/AEM.68.6.2950-2958.2002","article-title":"Microtiter plate assay for assessment of Listeria monocytogenes biofilm formation","volume":"68","author":"DJORDJEVIC D.","year":"2002","journal-title":"Applied and Environmental Microbiology"},{"key":"ref9","doi-asserted-by":"crossref","DOI":"10.1155\/2021\/1059274","article-title":"The use of essential oils and their isolated compounds for the treatment of oral candidiasis: a literature review","volume":"2021","author":"FERREIRA E.S.","year":"2021","journal-title":"Evidence-Based Complementary and Alternative Medicine"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1155\/2015\/147804","article-title":"DEOs scientific evidence for the use of natural products in the treatment of oral candidiasis exist? 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Reference method for mroth dilution antifungal susceptibility testing of yeasts","year":"2002","edition":"2nd"},{"issue":"24","key":"ref47","doi-asserted-by":"crossref","first-page":"6501","DOI":"10.1016\/j.bmc.2017.10.033","article-title":"Synthesis, antifungal and antitumor activity of two new types of imidazolin-2-ones","volume":"25","author":"WEI S.","year":"2017","journal-title":"Bioorganic & Medicinal Chemistry"}],"container-title":["Brazilian Journal of Biology"],"original-title":["Potencial bioativo do \u00f3leo essencial e extrato de Eugenia luschnathiana: atividade antif\u00fangica contra esp\u00e9cies de Candida isoladas de pacientes oncol\u00f3gicos"],"link":[{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_pdf&pid=S1519-69842024000101260&tlng=en","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,9,13]],"date-time":"2024-09-13T13:59:16Z","timestamp":1726235956000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_arttext&pid=S1519-69842024000101260&tlng=en"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024]]},"references-count":47,"alternative-id":["S1519-69842024000101260"],"URL":"https:\/\/doi.org\/10.1590\/1519-6984.286419","relation":{},"ISSN":["1678-4375","1519-6984"],"issn-type":[{"value":"1678-4375","type":"electronic"},{"value":"1519-6984","type":"print"}],"subject":[],"published":{"date-parts":[[2024]]},"article-number":"e286419"}}