{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T15:05:42Z","timestamp":1753887942104,"version":"3.41.2"},"reference-count":37,"publisher":"FapUNIFESP (SciELO)","license":[{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Braz. J. Biol."],"abstract":"<jats:p>Abstract The objective was to isolate and identify clinical species of Candida parapsilosis from the oral cavity of individuals without any oral alterations, such as oral candidiasis. Subsequently, the sensitivity of these strains to eugenol and nystatin was evaluated. To this end, the Minimum Inhibitory Concentration (MIC), Minimum Fungicidal Concentration (MFC), microbial growth death curve, and inhibition of virulence factors through micromorphology were determined. Seven strains were selected, six of them were clinical isolates of C. parapsilosis, and one was the American Type Culture Collection (ATCC\u00ae) 22019\u2122 standard strain. The MIC was determined to be 256 \u03bcg\/mL, which coincided with the MFC in 100% of the tested strains. Micromorphology study demonstrated inhibition of virulence structures such as pseudo-hyphae and blastoconidia at all three analyzed concentrations. The microbial death curve showed a fungicidal effect after 24 hours of experimentation in the studied strains. Therefore, eugenol constitutes a promising molecule for the treatment of oral fungal infections.<\/jats:p>","DOI":"10.1590\/1519-6984.286707","type":"journal-article","created":{"date-parts":[[2025,5,2]],"date-time":"2025-05-02T11:03:37Z","timestamp":1746183817000},"source":"Crossref","is-referenced-by-count":0,"title":["Antifungal effect of eugenol on Candida parapsilosis strains isolated from the oral cavity of healthy individuals"],"prefix":"10.1590","volume":"85","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4539-2007","authenticated-orcid":false,"given":"J. K.","family":"Oliveira-J\u00fanior","sequence":"first","affiliation":[{"name":"Universidade Federal da Para\u00edba,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7308-6779","authenticated-orcid":false,"given":"M. A. C.","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Centro Universit\u00e1rio Santa Maria,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1106-5259","authenticated-orcid":false,"given":"R. V.","family":"Sousa","sequence":"additional","affiliation":[{"name":"Centro Universit\u00e1rio Santa Maria,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8944-852X","authenticated-orcid":false,"given":"C. L.","family":"Drumond","sequence":"additional","affiliation":[{"name":"Centro Universit\u00e1rio Santa Maria,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7171-9504","authenticated-orcid":false,"given":"C. M.","family":"Soares","sequence":"additional","affiliation":[{"name":"Universidade Federal da Para\u00edba,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2647-2594","authenticated-orcid":false,"given":"D. F.","family":"Silva","sequence":"additional","affiliation":[{"name":"Universidade Federal da Para\u00edba,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3763-8537","authenticated-orcid":false,"given":"J. P.","family":"Sousa","sequence":"additional","affiliation":[{"name":"Universidade Federal da Para\u00edba,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2808-7836","authenticated-orcid":false,"given":"S. A.","family":"Brito","sequence":"additional","affiliation":[{"name":"Universidade Regional do Cariri,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2454-9116","authenticated-orcid":false,"given":"G. M. T.","family":"Silva","sequence":"additional","affiliation":[{"name":"Cirurgi\u00e3o-dentista,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2057-4821","authenticated-orcid":false,"given":"F. Q. S.","family":"Guerra","sequence":"additional","affiliation":[{"name":"Universidade Federal da Para\u00edba,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9547-0886","authenticated-orcid":false,"given":"E. O.","family":"Lima","sequence":"additional","affiliation":[{"name":"Universidade Federal da Para\u00edba,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","published-online":{"date-parts":[[2025]]},"reference":[{"issue":"1","key":"ref1","doi-asserted-by":"crossref","first-page":"11","DOI":"10.4103\/0973-029X.203793","article-title":"An in vitro study of isolation of candidal strains in oral squamous cell carcinoma patients undergoing radiation therapy and quantitative analysis of the various strains using CHROMagar","volume":"21","author":"AINAPUR M.M.A.R.","year":"2017","journal-title":"Journal of Oral and Maxillofacial Pathology"},{"issue":"1","key":"ref2","doi-asserted-by":"crossref","first-page":"57","DOI":"10.5380\/cep.v31i1.32700","article-title":"Atividade antimicrobiana de m\u00e9is produzidos por melipon\u00edneos nativos do Paran\u00e1 (Brasil)","volume":"31","author":"BORSATO D.M.","year":"2013","journal-title":"Boletim do Centro de Pesquisa de Processamento de Alimentos"},{"issue":"4","key":"ref3","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1111\/ipd.13056","article-title":"Lesion sterilization and tissue repair with chloramphenicol, tetracyline, zinc oxide\/eugenol paste versus conventional pulpectomy: a 36-month randomized controlled trial","volume":"33","author":"CASTRO M.","year":"2023","journal-title":"International Journal of Paediatric Dentistry"},{"issue":"3","key":"ref4","doi-asserted-by":"crossref","first-page":"341","DOI":"10.4034\/PBOCI.2011.113.06","article-title":"Screening da atividade antif\u00fangica de \u00f3leos essenciais sobre cepas de candida","volume":"11","author":"CASTRO R.D.","year":"2011","journal-title":"Pesquisa Brasileira em Odontopediatria e Clinica Integrada"},{"issue":"12","key":"ref5","article-title":"Candidemia cases caused by Candida parapsilosis complex species: epidemiology and antifungal susceptibility of strains","volume":"69","author":"CETINKAYA O.","year":"2023","journal-title":"Clinical Laboratory"},{"issue":"4","key":"ref6","doi-asserted-by":"crossref","first-page":"202","DOI":"10.4103\/jispcd.JISPCD_138_17","article-title":"An In vitro study to evaluate the effect of Eugenol-free and Eugenol-containing temporary cements on the bond strength of resin cement and considering time as a factor","volume":"7","author":"CHILUKA L.","year":"2017","journal-title":"Journal of International Society of Preventive & Community Dentistry"},{"edition":"3rd","year":"2010","series-title":"M27: reference method for broth dilution antifungal susceptibility testing of yeasts.","key":"ref7"},{"issue":"5","key":"ref8","doi-asserted-by":"crossref","first-page":"441","DOI":"10.2174\/1567201819666220418104042","article-title":"Oral candidiasis and novel therapeutic strategies: antifungals, phytotherapy, probiotics, and photodynamic therapy","volume":"20","author":"CONTALDO M.","year":"2023","journal-title":"Current Drug Delivery"},{"issue":"10","key":"ref9","doi-asserted-by":"crossref","first-page":"3776","DOI":"10.1128\/JCM.40.10.3776-3781.2002","article-title":"Optimal testing conditions for determining MICs and minimum fungicidal concentrations of new and established antifungal agents for uncommon molds: NCCLS collaborative study","volume":"40","author":"ESPINEL-INGROFF A.","year":"2002","journal-title":"Journal of Clinical Microbiology"},{"issue":"1","key":"ref10","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1111\/mve.12259","article-title":"Acaricidal activity of essential oil of Syzygium aromaticum, hydrolate and eugenol formulated or free on larvae and engorged females of Rhipicephalus microplus","volume":"32","author":"FERREIRA F.M.","year":"2017","journal-title":"Medical and Veterinary Entomology"},{"issue":"8","key":"ref11","doi-asserted-by":"crossref","first-page":"6422","DOI":"10.3390\/molecules16086422","article-title":"Alkylphenol activity against Candida spp. and Microsporum canis: a focus on the antifungal activity of Thymol, Eugenol and o-methyl derivatives","volume":"16","author":"FONTENELLE R.O.","year":"2011","journal-title":"Molecules"},{"issue":"8","key":"ref12","doi-asserted-by":"crossref","first-page":"798","DOI":"10.3390\/jof9080798","article-title":"Candida parapsilosis sensu stricto antifungal resistance mechanisms and associated epidemiology","volume":"9","author":"FRANCONI I.","year":"2023","journal-title":"Journal of Fungi"},{"issue":"1","key":"ref13","article-title":"Antifungal activity of essential oils against Candida albicans strains isolated from users of dental prostheses","volume":"2017","author":"FREIRE J.C.P.","year":"2017","journal-title":"Evidence-Based Complementary and Alternative Medicine"},{"issue":"1","key":"ref14","doi-asserted-by":"crossref","first-page":"96","DOI":"10.2174\/1874285801105010096","article-title":"Inhibition of growth of highly resistant bacterial and fungal pathogens by a natural product","volume":"5","author":"HAFIDH R.R.","year":"2011","journal-title":"The Open Microbiology Journal"},{"issue":"6","key":"ref15","doi-asserted-by":"crossref","first-page":"1467","DOI":"10.1590\/S0100-40422009000600020","article-title":"Antioxidant capacity of eugenol derivatives","volume":"32","author":"HIDALGO M.E.","year":"2009","journal-title":"Qu\u00edmica Nova"},{"issue":"7","key":"ref16","doi-asserted-by":"crossref","first-page":"1027","DOI":"10.1590\/S0074-02762002000700017","article-title":"Screening of some plants used in the Brazilian folk medicine for the treatment of infectious diseases","volume":"97","author":"HOLETZ F.B.","year":"2002","journal-title":"Memorias do Instituto Oswaldo Cruz"},{"key":"ref17","series-title":"Atlas of clinical fungi.","author":"HOOG G.S.","year":"2001","edition":"2nd"},{"issue":"2","key":"ref18","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1111\/1758-2229.13053","article-title":"In it together: Candida-bacterial oral biofilms and therapeutic strategies","volume":"14","author":"HWANG G.","year":"2022","journal-title":"Environmental Microbiology Reports"},{"issue":"11","key":"ref19","doi-asserted-by":"crossref","first-page":"6479","DOI":"10.1007\/s00784-022-04716-2","article-title":"Resistance profiles to antifungal agents in Candida albicans isolated from human oral cavities: systematic review and meta-analysis","volume":"26","author":"KESSLER S.Q.S.","year":"2022","journal-title":"Clinical Oral Investigations"},{"issue":"5","key":"ref20","doi-asserted-by":"crossref","first-page":"1207","DOI":"10.1128\/AAC.42.5.1207","article-title":"Influence of test conditions on antifungal time-kill curve results: proposal for standardized methods","volume":"42","author":"KLEPSER M.E.","year":"1998","journal-title":"Antimicrobial Agents and Chemotherapy"},{"key":"ref21","series-title":"The yeast: a taxonomic study.","author":"KURTZMANN C.P.","year":"1998","edition":"4th"},{"issue":"3","key":"ref22","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1111\/j.1439-0507.2009.01691.x","article-title":"The changing face of epidemiology of invasive fungal disease in Europe","volume":"52","author":"LASS-FL\u00d6RL C.","year":"2009","journal-title":"Mycoses"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1186\/1472-6882-11-119","article-title":"In vitro activities of natural products against oral Candida isolates from denture wearers","volume":"11","author":"MARCOS-ARIAS C.","year":"2011","journal-title":"BMC Complementary and Alternative Medicine"},{"issue":"6","key":"ref24","doi-asserted-by":"crossref","first-page":"1391","DOI":"10.3390\/microorganisms11061391","article-title":"Candida albicans Adhesins Als1 and Hwp1 modulate interactions with Streptococcus mutans","volume":"11","author":"MARTORANO-FERNANDES L.","year":"2023","journal-title":"Microorganisms"},{"issue":"2","key":"ref25","doi-asserted-by":"crossref","first-page":"300","DOI":"10.4103\/0976-9668.159983","article-title":"Oral Candidal and Streptococcal carriage in Down syndrome patients","volume":"6","author":"MOHIDDIN G.","year":"2015","journal-title":"Journal of Natural Science, Biology, and Medicine"},{"key":"ref26","doi-asserted-by":"crossref","DOI":"10.1016\/j.micpath.2020.104477","article-title":"Are Candida albicans isolates from the oral cavity of HIV-infected patients more virulent than from non-HIV-infected patients? Systematic review and meta-analysis","volume":"149","author":"ORLANDINI R.K.","year":"2020","journal-title":"Microbial Pathogenesis"},{"issue":"1","key":"ref27","doi-asserted-by":"crossref","DOI":"10.1155\/2014\/505204","article-title":"Effect of eugenol on cell surface hydrophobicity, adhesion, and biofilm of Candida tropicalis and Candida dubliniensis isolated from oral cavity of HIV-infected patients","volume":"2014","author":"PAULA S.B.","year":"2014","journal-title":"Evidence-Based Complementary and Alternative Medicine"},{"issue":"5","key":"ref28","doi-asserted-by":"crossref","first-page":"1181","DOI":"10.1093\/jac\/dks019","article-title":"Epidemiology, species distribution and in vitro antifungal susceptibility of fungaemia in a Spanish multicentre prospective survey","volume":"67","author":"PEM\u00c1N J.","year":"2012","journal-title":"The Journal of Antimicrobial Chemotherapy"},{"issue":"1","key":"ref29","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/j.pestbp.2010.05.004","article-title":"Chemical and antimicrobial studies of monoterpene: citral","volume":"98","author":"SADDIQ A.A.","year":"2010","journal-title":"Pesticide Biochemistry and Physiology"},{"key":"ref30","series-title":"Avan\u00e7os em ci\u00eancia e tecnologia de alimentos.","first-page":"59","volume-title":"Propriedades funcionais do eugenol e sua aplica\u00e7\u00e3o em alimentos","volume":"4","author":"SANTANA M.S.","year":"2021"},{"issue":"4","key":"ref31","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1590\/S1517-83822004000300001","article-title":"Composition and antimicrobial activity of essential oils from aromatic plants used in Brazil","volume":"35","author":"SARTORATTO A.","year":"2004","journal-title":"Brazilian Journal of Microbiology"},{"issue":"8","key":"ref32","first-page":"DC09","article-title":"Isolation and speciation of Candida in type II diabetic patients using CHROM Agar: a microbial study","volume":"11","author":"SHARMA U.","year":"2017","journal-title":"Journal of Clinical and Diagnostic Research"},{"year":"2004","series-title":"Micologia m\u00e9dica \u00e0 luz de autores contempor\u00e2neos.","author":"SIDRIM J.J.C.","key":"ref33"},{"issue":"1","key":"ref34","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1007\/s12281-011-0081-7","article-title":"Epidemiology of invasive fungal infections in Latin America","volume":"6","author":"SIFUENTES-OSORNIO J.","year":"2012","journal-title":"Current Fungal Infection Reports"},{"issue":"1","key":"ref35","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1016\/j.oooo.2014.03.017","article-title":"Evaluation of different treatment methods against denture stomatitis: a randomized clinical study","volume":"118","author":"TAY L.Y.","year":"2014","journal-title":"Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology"},{"issue":"4","key":"ref36","doi-asserted-by":"crossref","first-page":"606","DOI":"10.1128\/CMR.00013-08","article-title":"Candida parapsilosis, an emerging fungal pathogen","volume":"21","author":"TROFA D.","year":"2008","journal-title":"Clinical Microbiology Reviews"},{"issue":"3","key":"ref37","doi-asserted-by":"crossref","first-page":"272","DOI":"10.1590\/S0037-86822007000300004","article-title":"Identifica\u00e7\u00e3o microbiol\u00f3gica e sensibilidade in vitro de Candida isoladas da cavidade oral de indiv\u00edduos HIV positivos","volume":"40","author":"WINGETER M.A.","year":"2007","journal-title":"Revista da Sociedade Brasileira de Medicina Tropical"}],"container-title":["Brazilian Journal of Biology"],"original-title":["Efeito antif\u00fangico do eugenol em cepas de Candida parapsilosis isoladas da cavidade oral de indiv\u00edduos saud\u00e1veis"],"link":[{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_pdf&pid=S1519-69842025000100167&tlng=en","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,5,2]],"date-time":"2025-05-02T11:03:57Z","timestamp":1746183837000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_arttext&pid=S1519-69842025000100167&tlng=en"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025]]},"references-count":37,"alternative-id":["S1519-69842025000100167"],"URL":"https:\/\/doi.org\/10.1590\/1519-6984.286707","relation":{},"ISSN":["1678-4375","1519-6984"],"issn-type":[{"type":"electronic","value":"1678-4375"},{"type":"print","value":"1519-6984"}],"subject":[],"published":{"date-parts":[[2025]]},"article-number":"e286707"}}