{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,8,2]],"date-time":"2025-08-02T16:47:06Z","timestamp":1754153226960,"version":"3.41.2"},"reference-count":36,"publisher":"FapUNIFESP (SciELO)","license":[{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100008786","name":"Universidad Nacional Mayor de San Marcos","doi-asserted-by":"crossref","award":["016238-2020"],"award-info":[{"award-number":["016238-2020"]}],"id":[{"id":"10.13039\/501100008786","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100008786","name":"Universidad Nacional Mayor de San Marcos","doi-asserted-by":"crossref","award":["B2010002m"],"award-info":[{"award-number":["B2010002m"]}],"id":[{"id":"10.13039\/501100008786","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Braz. J. Biol."],"abstract":"<jats:p>Abstract The assessment of lymphocyte populations allows the monitoring, follow-up and treatment of patients with various infectious diseases. Acute respiratory syndrome caused by coronavirus (SARS-CoV-2), can lead to a decrease in circulating lymphocytes and cause disease progression and severity. We evaluated the association of CD4+\/CD8+ T lymphocyte with COVID-19 disease severity in moderate and severe patients. 136 patients with COVID-19 from the moderate group (MG) and severe group (SG) were compared with a healthy group or control group (CG). CD3+, CD4+ and CD8+ T lymphocytes were quantified by flow cytometry and correlated to CD4+\/CD8+ with sex, age, markers of inflammation (ferritin, C-reactive protein and D-dimer), lung damage and oxygen saturation. CD4+ and CD8+ T lymphocytes were significantly decreased in MG and SG (p&lt;0.0001). The CD4+\/CD8+ ratio showed association in MG with age (r=0.31; p=0.056), with lung damage (r=0.4; p=0.03) and in SG with total proteins (r=0.59; p=0.04) and D-dimer (r=0.57; p=0.03). The CD4+\/CD8+ ratio is associated with disease severity, significantly affecting the cellular immune response.<\/jats:p>","DOI":"10.1590\/1519-6984.289932","type":"journal-article","created":{"date-parts":[[2025,7,22]],"date-time":"2025-07-22T15:35:16Z","timestamp":1753198516000},"source":"Crossref","is-referenced-by-count":0,"title":["CD4+\/CD8+ lymphocyte ratio as a biomarker of morbidity and severity in patients with moderate and severe COVID-19"],"prefix":"10.1590","volume":"85","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2142-7575","authenticated-orcid":false,"given":"D.","family":"Torres-Gonzales","sequence":"first","affiliation":[{"name":"Universidad Nacional Mayor de San Marcos,  Per\u00fa; Hospital Nacional Guillermo Almenara Irigoyen,  Per\u00fa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9759-288X","authenticated-orcid":false,"given":"E.","family":"Colona-Vallejos","sequence":"additional","affiliation":[{"name":"Universidad Nacional Mayor de San Marcos,  Per\u00fa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7425-7453","authenticated-orcid":false,"given":"L.","family":"Alzamora-Gonzales","sequence":"additional","affiliation":[{"name":"Universidad Nacional Mayor de San Marcos,  Per\u00fa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9982-3263","authenticated-orcid":false,"given":"G.","family":"Tineo Pozo","sequence":"additional","affiliation":[{"name":"Universidad Nacional Mayor de San Marcos,  Per\u00fa; Hospital Nacional Guillermo Almenara Irigoyen,  Per\u00fa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5040-0641","authenticated-orcid":false,"given":"E.","family":"Chamorro Chirinos","sequence":"additional","affiliation":[{"name":"Hospital Nacional Guillermo Almenara Irigoyen,  Per\u00fa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-4140-8914","authenticated-orcid":false,"given":"C.","family":"Lorenzo Quito","sequence":"additional","affiliation":[{"name":"Hospital Nacional Guillermo Almenara Irigoyen,  Per\u00fa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0413-0104","authenticated-orcid":false,"given":"M.","family":"Aliaga Zamudio","sequence":"additional","affiliation":[{"name":"Universidad Nacional Mayor de San Marcos,  Per\u00fa; Hospital Nacional Guillermo Almenara Irigoyen,  Per\u00fa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3173-9425","authenticated-orcid":false,"given":"E.","family":"Cambillo Moyano","sequence":"additional","affiliation":[{"name":"Universidad Nacional Mayor de San Marcos,  Per\u00fa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4713-5511","authenticated-orcid":false,"given":"E. E.","family":"Aguirre-Siancas","sequence":"additional","affiliation":[{"name":"Universidad Nacional Mayor de San Marcos,  Per\u00fa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5340-7168","authenticated-orcid":false,"given":"E.","family":"Ruiz Ramirez","sequence":"additional","affiliation":[{"name":"Universidad Nacional Mayor de San Marcos,  Per\u00fa"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","published-online":{"date-parts":[[2025]]},"reference":[{"key":"ref1","article-title":"D-dimer and HbA1c levels findings in COVID-19 Iraqi patients","volume":"84","author":"AL-JUBURY K.S.K.","year":"2024","journal-title":"Brazilian Journal of Biology = Revista Brasileira de Biologia"},{"issue":"6","key":"ref2","first-page":"854","article-title":"North American AIDS cohort collaboration on research and design (NA-ACCORD) of the international epidemiology databases to evaluate AIDS (IeDEA). CD4\/CD8 ratio and cancer risk among adults with HIV","volume":"114","author":"CASTILHO J.L.","year":"2022","journal-title":"Journal of the National Cancer Institute"},{"issue":"1","key":"ref3","doi-asserted-by":"crossref","first-page":"86","DOI":"10.35434\/rcmhnaaa.2021.14Sup1.1181","article-title":"Ferritina s\u00e9rica en el pron\u00f3stico de mortalidad en pacientes con neumon\u00eda severa por SARS-CoV-2","volume":"14","author":"CASTILLO-RIVERA E.","year":"2021","journal-title":"Revista del Cuerpo M\u00e9dico Hospital Nacional Almanzor Aguinaga Asenjo"},{"issue":"3","key":"ref4","first-page":"S416","article-title":"\u00cdndice de saturaci\u00f3n y fracci\u00f3n inspirada de ox\u00edgeno en COVID-19","volume":"61","author":"CASTRO-ARELLANO S.B.","year":"2023","journal-title":"Revista Medica del Instituto Mexicano del Seguro Social"},{"key":"ref5","article-title":"Knowledge, behavior and practices of the population during the pandemic against the transmission of SARS-CoV-2 in the state of Alagoas, Brazil","volume":"84","author":"CARVALHO-NETO A.P.M.","year":"2024","journal-title":"Brazilian Journal of Biology = Revista Brasileira de Biologia"},{"issue":"10","key":"ref6","doi-asserted-by":"crossref","first-page":"878","DOI":"10.1136\/thx.2009.133280","article-title":"Severity assessment tools for predicting mortality in hospitalised patients with community-acquired pneumonia: systematic review and meta-analysis","volume":"65","author":"CHALMERS J.D.","year":"2010","journal-title":"Thorax"},{"issue":"5","key":"ref7","doi-asserted-by":"crossref","first-page":"2620","DOI":"10.1172\/JCI137244","article-title":"Clinical and immunological features of severe and moderate coronavirus disease 2019","volume":"130","author":"CHEN G.","year":"2020","journal-title":"The Journal of Clinical Investigation"},{"issue":"3","key":"ref8","doi-asserted-by":"crossref","DOI":"10.1111\/sji.13125","article-title":"High CD4-to-CD8 ratio identifies an at-risk population susceptible to lethal COVID-19","volume":"95","author":"DE ZUANI M.","year":"2022","journal-title":"Scandinavian Journal of Immunology"},{"issue":"1","key":"ref9","first-page":"57","article-title":"Ferritina como factor pron\u00f3stico en pacientes con s\u00edndrome de inmunodeficiencia adquirida","volume":"16","author":"FRANCO L.Y.","year":"2000","journal-title":"Revue de M\u00e9decine Interne"},{"issue":"15","key":"ref10","doi-asserted-by":"crossref","first-page":"458","DOI":"10.15585\/mmwr.mm6915e3","article-title":"Hospitalization rates and characteristics of patients hospitalized with laboratory-confirmed Coronavirus disease 2019 - COVID-NET, 14 States, March 1-30, 2020","volume":"69","author":"GARG S.","year":"2020","journal-title":"MMWR. Morbidity and Mortality Weekly Report"},{"key":"ref11","doi-asserted-by":"crossref","DOI":"10.1155\/2014\/267450","article-title":"Establishment of normal reference intervals for CD3(+), CD4(+), CD8(+), and CD4(+) to CD8(+) ratio of T lymphocytes in HIV negative adults from university of Gondar Hospital, North West Ethiopia","volume":"2014","author":"GIZE A.","year":"2014","journal-title":"AIDS Research and Treatment"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1016\/j.cca.2020.06.033","article-title":"Hyperferritinemia in critically ill COVID-19 patients: is ferritin the product of inflammation or a pathogenic mediator?","volume":"509","author":"G\u00d3MEZ-PASTORA J.","year":"2020","journal-title":"Clinica Chimica Acta"},{"issue":"1","key":"ref13","first-page":"8","article-title":"Prote\u00edna C reactiva de ingreso como predictor precoz en la severidad por Covid-19 en un centro de salud de referencia del Per\u00fa","volume":"89","author":"GUERRA W.H.","year":"2023","journal-title":"Revista M\u00e9dica de Rosario"},{"issue":"1","key":"ref14","first-page":"24","article-title":"Inflamaci\u00f3n y da\u00f1o pulmonar en infecci\u00f3n por COVID-19","volume":"7","author":"GUTI\u00c9RREZ C.A.","year":"2023","journal-title":"Revista del Instituto de Salud P\u00fablica de Chile"},{"issue":"1","key":"ref15","article-title":"La edad como variable asociada a la gravedad en pacientes con la COVID-19","volume":"51","author":"HERRERA C.C.","year":"2022","journal-title":"Revista Cubana de Medicina Militar"},{"issue":"10223","key":"ref16","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1016\/S0140-6736(20)30183-5","article-title":"Clinical features of patients infected with 2019 novel coronavirus in Wuhan, China","volume":"395","author":"HUANG C.","year":"2020","journal-title":"Lancet"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/j.pupt.2015.08.003","article-title":"Interaction between TRPA1 and TRPV1: synergy on pulmonary sensory nerves","volume":"35","author":"LEE L.Y.","year":"2015","journal-title":"Pulmonary Pharmacology & Therapeutics"},{"issue":"2","key":"ref18","doi-asserted-by":"crossref","DOI":"10.1016\/j.bjid.2021.101569","article-title":"Serum ferritin at admission in hospitalized COVID-19 patients as a predictor of mortality","volume":"25","author":"LINO K.","year":"2021","journal-title":"The Brazilian Journal of Infectious Diseases"},{"issue":"6512","key":"ref19","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1126\/science.abd3871","article-title":"Selective and cross-reactive SARS-CoV-2 T cell epitopes in unexposed humans","volume":"370","author":"MATEUS J.","year":"2020","journal-title":"Science"},{"issue":"2","key":"ref20","doi-asserted-by":"crossref","first-page":"262","DOI":"10.1097\/QAI.0b013e3181e9be6b","article-title":"Serious fatal and nonfatal non-AIDS-defining illnesses in Europe","volume":"55","author":"MOCROFT A.","year":"2010","journal-title":"Journal of Acquired Immune Deficiency Syndromes"},{"issue":"2","key":"ref21","first-page":"118","article-title":"Interpretaci\u00f3n cl\u00ednica del conteo de linfocitos T CD4 positivos en la infecci\u00f3n por VIH","volume":"52","author":"NODA A.","year":"2024","journal-title":"Revista Cubana de Medicina"},{"issue":"9","key":"ref22","doi-asserted-by":"crossref","first-page":"1281","DOI":"10.7150\/ijms.46614","article-title":"Factors associated with death outcome in patients with severe coronavirus disease-19 (COVID-19): a case-control study","volume":"17","author":"PAN F.","year":"2020","journal-title":"International Journal of Medical Sciences"},{"issue":"2","key":"ref23","first-page":"46","article-title":"CD4\/CD8 Ratio as morbility marker in HIV (+) patients","volume":"73","author":"PEIRANO F.","year":"2017","journal-title":"Boletin del Hospital Vina del Mar; Publicacion de la Fundacion Cientifica \u201cLucas Sierra\u201d"},{"year":"2020","series-title":"Resoluci\u00f3n Ministerial n\u00ba 193-2020. Prevenci\u00f3n, Diagn\u00f3stico y Tratamiento de personas afectadas por COVID-19 en el Per\u00fa.","key":"ref24"},{"issue":"6","key":"ref25","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1080\/10408363.2020.1770685","article-title":"Biomarkers associated with COVID-19 disease progression","volume":"57","author":"PONTI G.","year":"2020","journal-title":"Critical Reviews in Clinical Laboratory Sciences"},{"issue":"15","key":"ref26","doi-asserted-by":"crossref","first-page":"762","DOI":"10.1093\/cid\/ciaa248","article-title":"Dysregulation of immune response in patients with coronavirus 2019 (COVID-19) in Wuhan, China","volume":"71","author":"QIN C.","year":"2020","journal-title":"Clinical Infectious Diseases"},{"issue":"4","key":"ref27","doi-asserted-by":"crossref","first-page":"1455","DOI":"10.1152\/physrev.00020.2020","article-title":"SARS-CoV-2 and COVID-19: from the bench to the bedside","volume":"100","author":"ROMAGNOLI S.","year":"2020","journal-title":"Physiological Reviews"},{"key":"ref28","article-title":"CD4\/CD8 ratio and CD8+ T-cell count as prognostic markers for non-AIDS mortality in people living with HIV. A systematic review and meta-analysis","volume":"15","author":"RON R.","year":"2024","journal-title":"Frontiers in Immunology"},{"issue":"4","key":"ref29","doi-asserted-by":"crossref","first-page":"996","DOI":"10.1016\/j.cell.2020.09.038","article-title":"Antigen-specific adaptive immunity to SARS-CoV-2 in acute COVID-19 and associations with age and disease severity","volume":"183","author":"RYDYZNSKI M.C.","year":"2020","journal-title":"Cell"},{"issue":"11","key":"ref30","doi-asserted-by":"crossref","first-page":"1061","DOI":"10.1001\/jama.2020.1585","article-title":"Clinical Characteristics of 138 hospitalized patients with 2019 novel coronavirus-infected pneumonia in Wuhan, China","volume":"323","author":"WANG D.","year":"2020","journal-title":"Journal of the American Medical Association"},{"issue":"7","key":"ref31","doi-asserted-by":"crossref","first-page":"803","DOI":"10.1111\/1440-1681.12769","article-title":"Anti-inflammatory and retinal protective effects of capsaicin on ischaemia-induced injuries through the release of endogenous somatostatin","volume":"44","author":"WANG J.","year":"2017","journal-title":"Clinical and Experimental Pharmacology & Physiology"},{"issue":"1","key":"ref32","doi-asserted-by":"crossref","first-page":"11636","DOI":"10.1038\/s41598-021-91150-1","article-title":"Association between D-dimer level and chest CT severity score in patients with SARS-COV-2 pneumonia","volume":"11","author":"WANG L.","year":"2021","journal-title":"Scientific Reports"},{"issue":"3","key":"ref33","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1016\/j.bja.2023.04.008","article-title":"Characterising biological mechanisms underlying ethnicity-associated outcomes in COVID-19 through biomarker trajectories: a multicentre registry analysis","volume":"131","author":"WAN Y.I.","year":"2023","journal-title":"British Journal of Anaesthesia"},{"issue":"5","key":"ref34","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1016\/S2213-2600(20)30079-5","article-title":"Clinical course and outcomes of critically ill patients with SARS-CoV-2 pneumonia in Wuhan, China: a single-centered, retrospective, observational study","volume":"8","author":"YANG X.","year":"2020","journal-title":"The Lancet. Respiratory Medicine"},{"key":"ref35","doi-asserted-by":"crossref","DOI":"10.56903\/kasmera.5137774","article-title":"Linfopenia como predictor de severidad en pacientes con infecci\u00f3n por SARS-CoV-2","volume":"51","author":"ZAVALA-YAMARTE A.","year":"2023","journal-title":"Kasmera"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/j.ijid.2020.04.086","article-title":"Lymphopenia is associated with severe coronavirus disease 2019 (COVID-19) infections: a systemic review and meta-analysis","volume":"96","author":"ZHAO Q.","year":"2020","journal-title":"International Journal of Infectious Diseases"}],"container-title":["Brazilian Journal of Biology"],"original-title":["Raz\u00e3o de linf\u00f3citos CD4+\/CD8+ como biomarcador de morbidade e gravidade em pacientes com COVID-19 moderada e grave"],"link":[{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_pdf&pid=S1519-69842025000100274&tlng=en","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,7,22]],"date-time":"2025-07-22T16:50:20Z","timestamp":1753203020000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_arttext&pid=S1519-69842025000100274&tlng=en"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025]]},"references-count":36,"alternative-id":["S1519-69842025000100274"],"URL":"https:\/\/doi.org\/10.1590\/1519-6984.289932","relation":{},"ISSN":["1678-4375","1519-6984"],"issn-type":[{"type":"electronic","value":"1678-4375"},{"type":"print","value":"1519-6984"}],"subject":[],"published":{"date-parts":[[2025]]},"article-number":"e289932"}}