{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T14:36:22Z","timestamp":1783694182580,"version":"3.55.0"},"reference-count":34,"publisher":"FapUNIFESP (SciELO)","license":[{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Braz. J. Biol."],"abstract":"<jats:p>Abstract Cerrado is Brazil's second largest biome, and its fauna and flora are rich in biodiversity. As a result, many plants are being studied to find new viable drugs. Among the species of interest is Hymenaea courbaril, commonly known as \"jatob\u00e1\". In this scenario, the extract's standardization and the execution of in vitro studies are crucial steps before investigating its applicability in human beings. This study aimed to obtain a standardized liquid extract of Hymenaea courbaril stem bark and analyze its phytochemical, antimicrobial, and cytotoxic profiles. The extract proved viable in RAW 264.7 cells and inhibited the bacterial growth of a methicillin-resistant Staphylococcus aureus (MRSA) clinical isolate. After High-Performance Liquid Chromatography (HPLC) screening, peaks suggestive of catechin and epicatechin were found. In silico analysis of these flavonoids indicated an LD50 of 10000 mg\/kg, fulfillment of Lipinski's criteria, and both were classified as druglike, with a safety level of 6. In conclusion, the standardized liquid extract of Hymenaea courbaril stem bark is non-toxic and rich in catechin and epicatechin. It has antimicrobial action against Staphylococcus aureus, opening up prospects for in vivo studies.<\/jats:p>","DOI":"10.1590\/1519-6984.290059","type":"journal-article","created":{"date-parts":[[2025,12,15]],"date-time":"2025-12-15T15:02:19Z","timestamp":1765810939000},"source":"Crossref","is-referenced-by-count":1,"title":["Assessment of cytotoxic and antimicrobial activity of Hymenaea courbaril L. stem barks extract"],"prefix":"10.1590","volume":"85","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6751-1097","authenticated-orcid":false,"given":"L. M.","family":"Gomes","sequence":"first","affiliation":[{"name":"Universidade Estadual de Goi\u00e1s,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N. K.","family":"Carmo","sequence":"additional","affiliation":[{"name":"Pontif\u00edcia Universidade Cat\u00f3lica de Goi\u00e1s,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4700-9646","authenticated-orcid":false,"given":"M. C. C.","family":"Cordeiro","sequence":"additional","affiliation":[{"name":"Universidade Federal de Goi\u00e1s,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8827-8274","authenticated-orcid":false,"given":"C. M.","family":"Gomes","sequence":"additional","affiliation":[{"name":"Pontif\u00edcia Universidade Cat\u00f3lica de Goi\u00e1s,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5043-4526","authenticated-orcid":false,"given":"M. N. G.","family":"Te\u00f3filo","sequence":"additional","affiliation":[{"name":"Universidade Estadual de Goi\u00e1s,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0737-2600","authenticated-orcid":false,"given":"J. A. M.","family":"Paula","sequence":"additional","affiliation":[{"name":"Universidade Estadual de Goi\u00e1s,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7185-6821","authenticated-orcid":false,"given":"A. M.","family":"Cardoso","sequence":"additional","affiliation":[{"name":"Pontif\u00edcia Universidade Cat\u00f3lica de Goi\u00e1s,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3564-6368","authenticated-orcid":false,"given":"C. A.","family":"Romano","sequence":"additional","affiliation":[{"name":"Universidade Federal de Goi\u00e1s,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4424-7692","authenticated-orcid":false,"given":"J. R.","family":"Paula","sequence":"additional","affiliation":[{"name":"Universidade Federal de Goi\u00e1s,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2183-3944","authenticated-orcid":false,"given":"L. L.","family":"Borges","sequence":"additional","affiliation":[{"name":"Universidade Estadual de Goi\u00e1s,  Brasil; Pontif\u00edcia Universidade Cat\u00f3lica de Goi\u00e1s,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"530","published-online":{"date-parts":[[2025]]},"reference":[{"issue":"W1","key":"ref1","doi-asserted-by":"crossref","first-page":"W257","DOI":"10.1093\/nar\/gky318","article-title":"ProTox-II: a webserver for the prediction of toxicity of chemicals","volume":"46","author":"BANERJEE P.","year":"2018","journal-title":"Nucleic Acids Research"},{"key":"ref2","doi-asserted-by":"crossref","DOI":"10.1093\/nar\/gkae303","article-title":"ProTox 3.0: a webserver for the prediction of toxicity of chemicals","author":"BANERJEE P.","year":"2024","journal-title":"Nucleic Acids Research"},{"issue":"1","key":"ref3","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1016\/j.jep.2013.05.052","article-title":"Phytochemical study guided by the myorelaxant activity of the crude extract, fractions and constituent from stem bark of Hymenaea courbaril L","volume":"149","author":"BEZERRA G.P.","year":"2013","journal-title":"Journal of Ethnopharmacology"},{"issue":"3","key":"ref4","doi-asserted-by":"crossref","first-page":"975","DOI":"10.1016\/j.jep.2012.03.031","article-title":"Screening of Brazilian medicinal plants for antiviral activity against rotavirus","volume":"141","author":"CEC\u00cdLIO A.B.","year":"2012","journal-title":"Journal of Ethnopharmacology"},{"issue":"2","key":"ref5","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1590\/1983-084X\/13_027","article-title":"An\u00e1lises das embalagens de plantas medicinais comercializadas em farm\u00e1cias e drogarias do munic\u00edpio de Iju\u00ed\/RS","volume":"17","author":"COLET C.F.","year":"2015","journal-title":"Revista Brasileira de Plantas Medicinais"},{"key":"ref6","series-title":"Farmacognosia.","author":"COSTA A.F.","year":"2001","edition":"3rd"},{"issue":"1","key":"ref7","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1186\/1472-6882-14-245","article-title":"Antifungal and cytotoxicity activities of the fresh xylem sap of Hymenaea courbaril L. and its major constituent fisetin","volume":"14","author":"COSTA M.P.","year":"2014","journal-title":"BMC Complementary and Alternative Medicine"},{"issue":"4","key":"ref8","doi-asserted-by":"crossref","first-page":"160","DOI":"10.5897\/JMPR2021.7091","article-title":"Antioxidant effect of Hymenaea courbaril L. 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