{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T05:48:01Z","timestamp":1793339281391,"version":"4.5.0"},"reference-count":37,"publisher":"FapUNIFESP (SciELO)","license":[{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Braz. J. Biol."],"abstract":"<jats:p>Abstract Important arboviruses for public health such as Dengue, Zika, and Chikungunya are transmitted by mosquito vectors, primarily Aedes aegypti and Aedes albopictus. The control of populations of these mosquitoes can be carried out by mechanical, chemical, and biological control. In view of the environmental impacts caused by synthetic chemical insecticides, the search for botanical larvicides with larvicidal potential has intensified. The objective of this work is to investigate the occurrence of acetylcholinesterase inhibition and histopathological alterations in Aedes aegypti and Aedes albopictus submitted to treatment with the ethanolic extract of the leaves of Acmella oleracea as possible mechanisms of larvicidal action. Larvae of Aedes aegypti and Aedes albopictus were exposed to treatment with the ethanolic extract of Acmella oleracea for an exposure period of 24 hours. After this time, larval mortality was recorded, and the larvae were separated in order to investigate the mechanisms of action. The ethanolic extract of Acmella oleracea had an LC50 of 29.15 \u00b5g.mL\u22121 and 16.00 \u00b5g.mL\u22121 against Aedes aegypti and Aedes albopictus, respectively. Significant reductions in acetylcholinesterase activity were observed in larvae treated with the ethanolic extract of Acmella oleracea, as well as histopathological changes in the midgut of treated larvae, including loss of integrity of the intestinal epithelium, vacuolation of the cell cytoplasm, and disruption of the peritrophic matrix. These results suggest that that the mechanism of action of the ethanolic extract of Acmella oleracea against Aedes larvae includes the potential to inhibit acetylcholinesterase and cause histopathological alterations in the midgut.<\/jats:p>","DOI":"10.1590\/1519-6984.290849","type":"journal-article","created":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T15:07:39Z","timestamp":1762528059000},"source":"Crossref","is-referenced-by-count":2,"title":["Inhibition of acetylcholinesterase and histopathological changes in Aedes aegypti and Aedes albopictus larvae exposed to ethanolic extract of Acmella oleracea (L.) R.K. Jansen (Asteraceae)"],"prefix":"10.1590","volume":"85","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9303-8643","authenticated-orcid":false,"given":"C. L. S.","family":"Cabe\u00e7a","sequence":"first","affiliation":[{"name":"Universidade Estadual de Maring\u00e1,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0964-4297","authenticated-orcid":false,"given":"M. A. P.","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Universidade Federal do Par\u00e1,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7323-8456","authenticated-orcid":false,"given":"A. C. S.","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Instituto Federal de Educa\u00e7\u00e3o, Ci\u00eancia e Tecnologia do Par\u00e1,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0746-2211","authenticated-orcid":false,"given":"D. R.","family":"Alves","sequence":"additional","affiliation":[{"name":"Universidade Estadual do Cear\u00e1,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2766-3790","authenticated-orcid":false,"given":"S. M.","family":"Morais","sequence":"additional","affiliation":[{"name":"Universidade Estadual do Cear\u00e1,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1459-7525","authenticated-orcid":false,"given":"V. L.","family":"Farias","sequence":"additional","affiliation":[{"name":"Instituto Federal de Educa\u00e7\u00e3o, Ci\u00eancia e Tecnologia do Cear\u00e1,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-2291-7018","authenticated-orcid":false,"given":"G. R.","family":"Silva","sequence":"additional","affiliation":[{"name":"Instituto Federal de Educa\u00e7\u00e3o, Ci\u00eancia e Tecnologia do Cear\u00e1,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8968-9611","authenticated-orcid":false,"given":"A. M.","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Instituto Federal de Educa\u00e7\u00e3o, Ci\u00eancia e Tecnologia do Cear\u00e1,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"530","published-online":{"date-parts":[[2025]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"852","DOI":"10.1016\/j.indcrop.2013.08.043","article-title":"Screening of phytochemical, physico-chemical and bioactivity of different parts of Acmella oleraceae Murr. (Asteraceae), a natural remedy for toothache","volume":"50","author":"ABEYSIRI G.R.P.I.","year":"2013","journal-title":"Industrial Crops and Products"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1016\/j.sajb.2018.05.008","article-title":"Larvicidal effect of hydroethanolic extract from the leaves of Acmella oleracea L. R. K. Jansen in Aedes aegypti and Culex quinquefasciatus.","volume":"117","author":"ARA\u00daJO I.F.","year":"2018","journal-title":"South African Journal of Botany"},{"key":"ref3","doi-asserted-by":"crossref","DOI":"10.1016\/j.bcab.2020.101550","article-title":"Larvicidal activity of the methanolic, hydroethanolic and hexanic extracts from Acmella oleracea, solubilized with silk fibroin, against Aedes aegypti.","volume":"24","author":"ARA\u00daJO I.F.","year":"2020","journal-title":"Biocatalysis and Agricultural Biotechnology"},{"key":"ref4","doi-asserted-by":"crossref","DOI":"10.1016\/j.microc.2020.105035","article-title":"Analytical and preparative chromatographic approaches for extraction of spilanthol from Acmella oleracea flowers","volume":"157","author":"BALIEIRO O.C.","year":"2020","journal-title":"Microchemical Journal"},{"issue":"1","key":"ref5","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1016\/j.bjp.2015.07.024","article-title":"Spilanthol: occurrence, extraction, chemistry and biological activities","volume":"26","author":"BARBOSA A.F.","year":"2016","journal-title":"Revista Brasileira de Farmacognosia"},{"issue":"1","key":"ref6","first-page":"37","article-title":"Estudo das altera\u00e7\u00f5es morfo-histol\u00f3gicas em larvas de Aedes aegypti (Diptera, Culicidae) submetidas ao extrato bruto etan\u00f3lico de Sapindus saponaria Lin (Sapindaceae)","volume":"35","author":"BARRETO C.F.","year":"2006","journal-title":"Revista de Patologia Tropical"},{"key":"ref7","series-title":"Atividade larvicida do extrato bruto de Swinglea glutinosa evidenciada pelas altera\u00e7\u00f5es morfohistol\u00f3gicas em larvas de Aedes aegypti (Diptera, Culicidae)","author":"CABRAL S.S.","year":"2015"},{"issue":"4","key":"ref8","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1007\/s13744-012-0050-z","article-title":"Morphological changes in the midgut of Aedes aegypti L. (diptera: Culicidae) larvae following exposure to an Annona coriacea (magnoliales: Annonaceae) extract","volume":"41","author":"COSTA M.","year":"2012","journal-title":"Neotropical Entomology"},{"issue":"2","key":"ref9","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1016\/0006-2952(61)90145-9","article-title":"A new and rapid colorimetric determination of acetylcholinesterase activity","volume":"7","author":"ELLMAN G.L.","year":"1961","journal-title":"Biochemical Pharmacology"},{"key":"ref10","doi-asserted-by":"crossref","DOI":"10.1016\/j.indcrop.2024.118818","article-title":"Green purification of Acmella oleracea extract by wiped-film short path molecular distillation enhances insecticidal activity against mosquito larvae","volume":"218","author":"FERRATI M.","year":"2024","journal-title":"Industrial Crops and Products"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.onehlt.2017.06.001","article-title":"Emerging arboviruses: why today?","volume":"4","author":"GOULD E.","year":"2017","journal-title":"One Health"},{"issue":"1","key":"ref12","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1016\/S1995-7645(11)60026-6","article-title":"Mosquito larvicidal and ovicidal properties of Eclipta alba (L.) hassk (asteraceae) against chikungunya vector, Aedes aegypti (Linn.) 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R.K. Jansen): a comparison between hydroponic and conventional cultivation using PCA and cluster analysis","volume":"332","author":"NASCIMENTO L.E.S.","year":"2020","journal-title":"Food Chemistry"},{"key":"ref20","doi-asserted-by":"crossref","DOI":"10.1016\/j.actatropica.2022.106747","article-title":"Extracellular extracts of antagonistic fungi, Trichoderma longibrachiatum and Trichoderma viride, as larvicides against dengue vectors, Aedes aegypti and Aedes albopictus.","volume":"238","author":"PERERA D.S.","year":"2023","journal-title":"Acta Tropica"},{"key":"ref21","series-title":"Laboratory methods in histotechnology.","author":"PROPHET E.B.","year":"1992"},{"key":"ref22","article-title":"Ethnopharmacological aspects of Acmella oleracea-a comprehensive review","volume":"299","author":"RANJAN R.K.","year":"2022","journal-title":"Journal of Ethnopharmacology"},{"issue":"9","key":"ref23","doi-asserted-by":"crossref","first-page":"913","DOI":"10.1016\/j.cropro.2010.05.008","article-title":"Mechanism of action of insecticidal secondary metabolites of plant origin","volume":"29","author":"RATTAN R.S.","year":"2010","journal-title":"Crop Protection (Guildford, Surrey)"},{"issue":"2","key":"ref24","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1006\/jipa.1998.4810","article-title":"Histopathological effects of tannic acid on the midgut epithelium of some aquatic diptera larvae","volume":"73","author":"REY D.","year":"1999","journal-title":"Journal of Invertebrate Pathology"},{"issue":"9","key":"ref25","doi-asserted-by":"crossref","first-page":"384","DOI":"10.4103\/1995-7645.290583","article-title":"Larvicidal efficacy of plant 12 extracts and isolated compounds from Annonaceae and Piperaceae against Aedes aegypti and Aedes albopictus.","volume":"13","author":"RODRIGUES A.M.","year":"2020","journal-title":"Asian Pacific Journal of Tropical Medicine"},{"key":"ref26","doi-asserted-by":"crossref","DOI":"10.1016\/j.indcrop.2021.113678","article-title":"Larvicidal activity of Annona mucosa Jacq. extract and main constituents rolliniastatin 1 and rollinicin against Aedes aegypti and Aedes albopictus.","volume":"169","author":"RODRIGUES A.M.","year":"2021","journal-title":"Industrial Crops and Products"},{"issue":"10","key":"ref27","doi-asserted-by":"crossref","first-page":"1965","DOI":"10.1007\/BF01013489","article-title":"Plant-insect coevolution and inhibition of acetylcholinesterase","volume":"14","author":"RYAN M.","year":"1988","journal-title":"Journal of Chemical Ecology"},{"issue":"2","key":"ref28","article-title":"Main mechanisms of insecticide resistance on insects","volume":"3","author":"SANT\u2019ANNA F.B.","year":"2009","journal-title":"Pubvet"},{"key":"ref29","series-title":"Alcaloides de plantas da fam\u00edlia Amaryllidaceae: isolamento caracteriza\u00e7\u00e3o e testes de inibi\u00e7\u00e3o de acetilcolinesterase.","author":"SILVA M.S.S.","year":"2009"},{"issue":"3","key":"ref30","first-page":"275","article-title":"Acetylenic 2-phenylethylamides and new isobutylamides from Acmella oleracea (L.) R. K. Jansen, a TAIPE-LAGOS, C.B. and NATAL, D., 2003. Abund\u00e2ncia de culic\u00eddeos em \u00e1rea metropolitana preservada e suas implica\u00e7\u00f5es epidemiol\u00f3gicas","volume":"37","author":"SIMAS N.K.","year":"2013","journal-title":"Revista de Saude Publica"},{"issue":"1","key":"ref31","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1590\/S0100-40422004000100009","article-title":"Produtos naturais para o controle da transmiss\u00e3o da dengue - Atividade larvicida de Myroxylon balsamum (\u00f3leo vermelho) e de terpenoides e fenilpropanoides","volume":"27","author":"SIMAS N.K.","year":"2004","journal-title":"Quimica Nova"},{"key":"ref32","doi-asserted-by":"crossref","DOI":"10.1016\/j.indcrop.2021.114027","article-title":"Spilanthol-rich essential oil obtained by microwave-assisted extraction from Acmella oleracea (L.) R.K. Jansen and its nanoemulsion: Insecticidal, cytotoxic and anti-inflammatory activities","volume":"172","author":"SPINOZZI E.","year":"2021","journal-title":"Industrial Crops and Products"},{"issue":"3","key":"ref33","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1590\/S0034-89102003000300002","article-title":"Abund\u00e2ncia de culic\u00eddeos em \u00e1rea metropolitana preservada e suas implica\u00e7\u00f5es epidemiol\u00f3gicas","volume":"37","author":"TAIPE-LAGOS C.B.","year":"2003","journal-title":"Revista de Sa\u00fade P\u00fablica"},{"issue":"4","key":"ref34","first-page":"309","article-title":"Changes in morphohistologic larvae of Aedes aegypti (Linnaeus, 1762) (Diptera, Culicidae) caused by tannins catechin isolated from the plant Magonia pubescens (Sapindaceae)","volume":"39","author":"VALOTTO C.F.B.","year":"2010","journal-title":"Revista de Patologia Tropical"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1016\/j.ecoenv.2017.01.026","article-title":"Comparative analysis of mosquito (Diptera: Culicidae: Aedes aegypti Liston) responses to the insecticide Temephos and plant derived essential oil derived from Piper betle L","volume":"139","author":"VASANTHA-SRINIVASAN P.","year":"2017","journal-title":"Ecotoxicology and Environmental Safety"},{"key":"ref36","series-title":"Guidelines for laboratory and field testing of mosquito larvicides","year":"2005"},{"issue":"2","key":"ref37","first-page":"391","article-title":"Estrat\u00e9gias de controle do Aedes aegypti: uma revis\u00e3o","volume":"25","author":"ZARA A.L.D.S.A.","year":"2016","journal-title":"Epidemiologia e Servi\u00e7os de Sa\u00fade : Revista do Sistema Unico de Sa\u00fade do Brasil"}],"container-title":["Brazilian Journal of Biology"],"original-title":["Inibi\u00e7\u00e3o da acetilcolinesterase e altera\u00e7\u00f5es histopatol\u00f3gicas em larvas de Aedes aegypti e Aedes albopictus expostas ao extrato etan\u00f3lico de Acmella oleracea (L.) R.K. Jansen (Asteraceae)"],"link":[{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_pdf&pid=S1519-69842025000100390&tlng=en","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T15:07:43Z","timestamp":1762528063000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_arttext&pid=S1519-69842025000100390&tlng=en"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025]]},"references-count":37,"alternative-id":["S1519-69842025000100390"],"URL":"https:\/\/doi.org\/10.1590\/1519-6984.290849","relation":{},"ISSN":["1678-4375","1519-6984"],"issn-type":[{"value":"1678-4375","type":"electronic"},{"value":"1519-6984","type":"print"}],"subject":[],"published":{"date-parts":[[2025]]},"article-number":"e290849"}}