{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T11:21:02Z","timestamp":1780485662012,"version":"3.54.1"},"reference-count":33,"publisher":"FapUNIFESP (SciELO)","license":[{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Braz. J. Biol."],"abstract":"<jats:p>Abstract The chemical response of callogenic suspension cultures of the plant Duroia saccifera was evaluated through elicitation by the endophytes Colletotrichum dianesei and Xylaria sp. Dried mycelia and methanolic extracts of dried mycelia from both fungi were used as elicitators. D. saccifera calli were multiplied in Murashige and Skoog medium with growth regulators, while endophytic fungi were cultivated in three different media: potato-dextrose (PD), czapek (CZ) or sabouraud (SB). Part of mycelia cultured in PD medium were dried and autoclaved. The remaining mycelia from the PD, and those from SB and CZ media were filtered, dried, weighed, and extracted with methanol (MeOH). The methanol fungal extracts used as elicitors were chemically analized by 1H nuclear magnetic resonance (1H-NMR) and thin layer chromatography (TLC) and their toxicity was evaluated using Artemia salina as a bioindicator. For elicitation, 100 mg of mycelium or 20 mg of fungal extract were used for 2 grams of friable callus. After 30 days, the elicited callogenic suspensions were filtered, weighed, dried, and extracted with methanol. The culture medium obtained from filtration was lyophilized and partitioned with ethyl acetate (EtOAc). Analyzes were performed using TLC and 1H-NMR. As results, it was observed that fungal extracts of C. dianesei and Xylaria sp. intensified the classes of metabolites already produced by the callus culture, with exception of the extract from Xylaria sp. grown in PD medium, which yielded the production of different aromatic substances did not present in the callus control extract. This fungal extract also exhibited high toxicity against Artemia salina. This suggests a correlation between the level of extract toxicity and the callus elicitation response.<\/jats:p>","DOI":"10.1590\/1519-6984.291007","type":"journal-article","created":{"date-parts":[[2025,5,12]],"date-time":"2025-05-12T11:11:26Z","timestamp":1747048286000},"source":"Crossref","is-referenced-by-count":1,"title":["Endophytic fungi as enhancers of secondary metabolite production in Duroia saccifera cell suspension"],"prefix":"10.1590","volume":"85","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1447-3661","authenticated-orcid":false,"given":"L. M.","family":"Lima","sequence":"first","affiliation":[{"name":"Instituto Nacional de Pesquisas da Amaz\u00f4nia,  Brasil; Instituto Nacional de Pesquisas da Amaz\u00f4nia,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-4467-0056","authenticated-orcid":false,"given":"R. R.","family":"Nunes","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Pesquisas da Amaz\u00f4nia,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-2080-2201","authenticated-orcid":false,"given":"L. I. T.","family":"Muniz","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Pesquisas da Amaz\u00f4nia,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8908-8576","authenticated-orcid":false,"given":"W. L.","family":"Silva","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Pesquisas da Amaz\u00f4nia,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3400-9508","authenticated-orcid":false,"given":"C. V.","family":"Nunez","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Pesquisas da Amaz\u00f4nia,  Brasil; Instituto Nacional de Pesquisas da Amaz\u00f4nia,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"530","published-online":{"date-parts":[[2025]]},"reference":[{"key":"ref1","article-title":"Duroia saccifera: in vitro germination, friable calli and identification of \u03b2-sitosterol and stigmasterol from the active extract against Mycobacterium tuberculosis.","volume":"71","author":"ALZATE-LOZANO S.A.","year":"2020","journal-title":"Rodrigu\u00e9sia"},{"key":"ref2","article-title":"Endophytic fungi: an overview on biotechnological and agronomic potential","volume":"84","author":"BOGAS A.C.","year":"2022","journal-title":"Brazilian Journal of Medical and Biological Research"},{"issue":"3","key":"ref3","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1007\/s11240-011-0051-3","article-title":"Polysaccharide elicitors enhance anthocyanin and phenolic acid accumulation in cell suspension cultures of Vitis vinifera.","volume":"108","author":"CAI Z.","year":"2012","journal-title":"Plant Cell, Tissue and Organ Culture"},{"key":"ref4","series-title":"Estudo fitoqu\u00edmico do extrato hex\u00e2nico das folhas de Duroia saccifera (Mart. ex Roem & Schult.) Hook. F ex Schumann (Rubiaceae)","author":"CONTRERAS-MEJIA J.A.","year":"2017"},{"issue":"4","key":"ref5","doi-asserted-by":"crossref","first-page":"1473","DOI":"10.1083\/jcb.105.4.1473","article-title":"Effects of cytochalasin and phalloidin on actin","volume":"105","author":"COOPER J.A.","year":"1987","journal-title":"The Journal of Cell Biology"},{"issue":"2","key":"ref6","first-page":"423","article-title":"Evaluation of the phytotoxic effect of extracts from endophytic fungi Colletotrichum dianesei and Xylaria sp. isolated from Palicourea corymbifera (Rubiaceae)","volume":"24","author":"DA SILVA W.L.","year":"2020","journal-title":"Agrociencia (Uruguay)"},{"key":"ref7","series-title":"Medicinal natural products: a biosynthetic approach.","author":"DEWICK P.M.","year":"2011","edition":"3rd"},{"key":"ref8","article-title":"Optimization of flavonoid production in plant cell culture of Thevetia peruviana elicited with methyl jasmonate and salicylic acid","volume":"64","author":"DUR\u00c1N M.D.L.","year":"2021","journal-title":"Brazilian Archives of Biology and Technology"},{"issue":"8","key":"ref9","doi-asserted-by":"crossref","first-page":"864","DOI":"10.3390\/jof9080864","article-title":"Antimicrobial and antioxidant activities of endophytic fungi associated with Arrabidaea chica (Bignoniaceae)","volume":"9","author":"GURGEL R.S.","year":"2023","journal-title":"Journal of Fungi"},{"key":"ref10","series-title":"Graphpad software","year":"1994"},{"issue":"1","key":"ref11","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1146\/annurev.phyto.34.1.387","article-title":"Microbial elicitors and their receptors in plants","volume":"34","author":"HAHN M.G.","year":"1996","journal-title":"Annual Review of Phytopathology"},{"issue":"6","key":"ref12","doi-asserted-by":"crossref","first-page":"901","DOI":"10.1016\/j.procbio.2011.06.012","article-title":"Culture filtrate of root endophytic fungus Piriformospora indica promotes the growth and lignan production of Linum album hairy root cultures","volume":"47","author":"KUMAR V.","year":"2012","journal-title":"Process Biochemistry"},{"issue":"2","key":"ref13","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1007\/s11240-017-1237-0","article-title":"Elicitor enhanced production of protoberberine alkaloids from in vitro cell suspension cultures of Tinospora cordifolia (Willd.) Miers ex Hook. F. & Thoms","volume":"130","author":"KUMAR P.","year":"2017","journal-title":"Plant Cell, Tissue and Organ Culture"},{"issue":"12","key":"ref14","doi-asserted-by":"crossref","first-page":"3276","DOI":"10.3390\/molecules23123276","article-title":"Effect of Aspergillus flavus fungal elicitor on the production of terpenoid indole alkaloids in Catharanthus roseus cambial meristematic cells","volume":"23","author":"LIANG C.","year":"2018","journal-title":"Molecules"},{"key":"ref15","series-title":"Studies in natural product chemistry 9.","first-page":"383","volume-title":"\u201cBenchtop\u201d bioassays for the discovery of bioactive natural products: an update.","author":"MCLAUGHLIN J.L.","year":"1991"},{"issue":"4","key":"ref16","doi-asserted-by":"crossref","first-page":"604","DOI":"10.1590\/1983-084X\/12_153","article-title":"Atividades biol\u00f3gicas de esp\u00e9cies amaz\u00f4nicas de Rubiaceae","volume":"17","author":"MESQUITA D.W.O.","year":"2015","journal-title":"Revista Brasileira de Plantas Medicinais"},{"issue":"5","key":"ref17","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1055\/s-2007-971236","article-title":"Brine shrimp: a convenient general bioassay for active plant constituents","volume":"45","author":"MEYER B.N.","year":"1982","journal-title":"Planta Medica"},{"issue":"1","key":"ref18","doi-asserted-by":"crossref","DOI":"10.1155\/2013\/209434","article-title":"Induction and analysis of the alkaloid mitragynine content of a Mitragyna speciosa suspension culture system upon elicitation and precursor feeding","volume":"2013","author":"MOHAMAD ZULDIN N.N.","year":"2013","journal-title":"The Scientific World Journal"},{"issue":"3","key":"ref19","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1111\/j.1399-3054.1962.tb08052.x","article-title":"A revised medium for rapid growth and bioassays with tobacco tissue cultures","volume":"15","author":"MURASHIGE T.","year":"1962","journal-title":"Physiologia Plantarum"},{"issue":"0","key":"ref20","doi-asserted-by":"crossref","DOI":"10.1590\/1678-4324-2017160542","article-title":"Endophytic fungi as novel resources of natural therapeutics","volume":"60","author":"RAJAMANIKYAM M.","year":"2017","journal-title":"Brazilian Archives of Biology and Technology"},{"issue":"6","key":"ref21","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1016\/j.tplants.2004.04.005","article-title":"Evolution of endophyte\u2013plant symbioses","volume":"9","author":"SAIKKONEN K.","year":"2004","journal-title":"Trends in Plant Science"},{"issue":"7","key":"ref22","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0236191","article-title":"Whole fungal elicitors boost paclitaxel biosynthesis induction in Corylus avellana cell culture","volume":"15","author":"SALEHI M.","year":"2020","journal-title":"PLoS One"},{"key":"ref23","doi-asserted-by":"crossref","DOI":"10.1590\/fst.49822","article-title":"Secondary metabolites in sorghum and its characteristics","volume":"42","author":"SETYORINI D.","year":"2022","journal-title":"Food Science and Technology"},{"key":"ref24","series-title":"Cultura de calos e suspens\u00e3o celular da esp\u00e9cie Duroia saccifera: estudo fitoqu\u00edmico, cin\u00e9tica de crescimento e avalia\u00e7\u00e3o das atividades biol\u00f3gicas.","author":"SOUSA A.B.S.","year":"2018"},{"key":"ref25","series-title":"Obten\u00e7\u00e3o de calos de Duroia saccifera Hook. F. (Rubiaceae) estudo qu\u00edmico e avalia\u00e7\u00e3o biol\u00f3gica dos seus extratos.","author":"SOUZA J.C.","year":"2016"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"269","DOI":"10.17648\/2446-4775.2018.550","article-title":"Produ\u00e7\u00e3o de metab\u00f3litos secund\u00e1rios por meio da cultura de tecidos vegetais","volume":"12","author":"SOUZA J.C.","year":"2018","journal-title":"Revista Fitos Eletr\u00f4nica"},{"issue":"4","key":"ref27","doi-asserted-by":"crossref","first-page":"448","DOI":"10.1039\/b100918o","article-title":"Endophytes: a rich source of functional metabolites","volume":"18","author":"TAN R.X.","year":"2001","journal-title":"Natural Product Reports"},{"issue":"19","key":"ref28","doi-asserted-by":"crossref","first-page":"3373","DOI":"10.3390\/plants12193373","article-title":"Fungal elicitation enhances vincristine and vinblastine yield in the embryogenic tissues of Catharanthus roseus.","volume":"12","author":"TONK D.","year":"2023","journal-title":"Plants"},{"key":"ref29","doi-asserted-by":"crossref","DOI":"10.3389\/fpls.2023.1248319","article-title":"Contribution of endophytes towards improving plant bioactive metabolites: a rescue option against red-taping of medicinal plants","volume":"14","author":"TSIPINANA S.","year":"2023","journal-title":"Frontiers in Plant Science"},{"issue":"18","key":"ref30","doi-asserted-by":"crossref","first-page":"6649","DOI":"10.1007\/s00253-021-11539-w","article-title":"Production of bioactive plant secondary metabolites through in vitro technologies-status and outlook","volume":"105","author":"WAWROSCH C.","year":"2021","journal-title":"Applied Microbiology and Biotechnology"},{"issue":"8","key":"ref31","doi-asserted-by":"crossref","first-page":"296","DOI":"10.1139\/cjm-2022-0194","article-title":"An endophytic fungus Schizophyllum commune isolated from Panax ginseng enhances hairy roots growth and ginsenoside biosynthesis","volume":"69","author":"XIE X.G.","year":"2023","journal-title":"Canadian Journal of Microbiology"},{"key":"ref32","first-page":"3874","article-title":"Secondary metabolites induction in Mammillaria huitzilopochtli (Cactaceae) and evaluation of the fungicidal activity","volume":"8","author":"ZEPEDA R.E.R.","year":"2009","journal-title":"African Journal of Biotechnology"},{"issue":"7-8","key":"ref33","doi-asserted-by":"crossref","first-page":"666","DOI":"10.1016\/S0141-0229(01)00309-X","article-title":"Selection of fungal elicitors to increase indole alkaloid accumulation in Catharanthus roseus suspension cell culture","volume":"28","author":"ZHAO J.","year":"2001","journal-title":"Enzyme and Microbial Technology"}],"container-title":["Brazilian Journal of Biology"],"original-title":["Fungos endof\u00edticos como potencializadores da produ\u00e7\u00e3o de metab\u00f3litos secund\u00e1rios na suspens\u00e3o celular de Duroia saccifera"],"link":[{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_pdf&pid=S1519-69842025000100180&tlng=en","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,5,12]],"date-time":"2025-05-12T11:11:33Z","timestamp":1747048293000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_arttext&pid=S1519-69842025000100180&tlng=en"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025]]},"references-count":33,"alternative-id":["S1519-69842025000100180"],"URL":"https:\/\/doi.org\/10.1590\/1519-6984.291007","relation":{},"ISSN":["1678-4375","1519-6984"],"issn-type":[{"value":"1678-4375","type":"electronic"},{"value":"1519-6984","type":"print"}],"subject":[],"published":{"date-parts":[[2025]]},"article-number":"e291007"}}