{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,20]],"date-time":"2026-03-20T14:01:49Z","timestamp":1774015309554,"version":"3.50.1"},"reference-count":53,"publisher":"FapUNIFESP (SciELO)","license":[{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Braz. J. Biol."],"abstract":"<jats:p>Abstract This study elucidates the antioxidant, anticancer, and antidiabetic properties of various extracts from Brassica oleracea var acephala (labeled K-29), specifically hexane (BraH), chloroform (BraC), ethyl acetate (BraE), and methanol (BraM) fractions. Antioxidant efficacy was determined through DPPH, ABTS, and FRAP assays, demonstrating a polarity-dependent increase in activity. Among the tested extracts, BraM exhibited most pronounced antioxidant activity, with IC50 values of 49.32 \u00b5g\/mL, 60.10 \u00b5g\/mL, and 67.73 \u00b5g\/mL in DPPH, ABTS, and FRAP assays, respectively, achieving inhibition rates of 92.14%, 90.33%, and 88.35%. BraE displayed moderate antioxidant potential (71.25 \u00b5g\/mL to 97.64 \u00b5g\/mL), while BraH exhibited the lowest activity (170.88 \u00b5g\/mL to 263.29 \u00b5g\/mL). Antiproliferative activity was assessed against A549 and HeLa cell lines, with BraC showing the most potent cytotoxic effects, as reflected by GI50 values of 65.09 \u00b5g\/mL and 37.00 \u00b5g\/mL for A549 and HeLa cells, respectively. The antidiabetic potential of K-29 was further evaluated via \u03b1-amylase and \u03b1-glucosidase inhibition assays, where the aqueous extract demonstrated the highest inhibition rates of 85.08% and 89.55%, respectively, followed by BraM (71.52% and 81.86%), while BraE exhibited minimal inhibitory activity. These results underscore the therapeutic promise of Brassica oleracea extracts as potent natural antioxidants, anticancer agents, and antidiabetic interventions.<\/jats:p>","DOI":"10.1590\/1519-6984.291259","type":"journal-article","created":{"date-parts":[[2026,3,20]],"date-time":"2026-03-20T11:49:24Z","timestamp":1774007364000},"source":"Crossref","is-referenced-by-count":0,"title":["Potent antioxidant and anticancer efficacy of Brassica oleracea var acephala extracts: a cytomorphological and antidiabetic assessment"],"prefix":"10.1590","volume":"85","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6161-1006","authenticated-orcid":false,"given":"S.","family":"Shafi","sequence":"first","affiliation":[{"name":"Chandigarh University,  India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3736-7610","authenticated-orcid":false,"given":"A. H.","family":"Bhat","sequence":"additional","affiliation":[{"name":"Chandigarh University,  India; Chandigarh University,  India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-9528-932X","authenticated-orcid":false,"given":"S. A.","family":"Shiekh","sequence":"additional","affiliation":[{"name":"Guru Nanak Dev University,  India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2835-9317","authenticated-orcid":false,"given":"G.","family":"Mukherjee","sequence":"additional","affiliation":[{"name":"Chandigarh University,  India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9723-086X","authenticated-orcid":false,"given":"C.","family":"Tuemmers","sequence":"additional","affiliation":[{"name":"Universidad Cat\u00f3lica de Temuco,  Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","published-online":{"date-parts":[[2025]]},"reference":[{"issue":"16","key":"ref1","doi-asserted-by":"crossref","DOI":"10.1016\/j.heliyon.2024.e34746","article-title":"Metabolomic insights and bioactive efficacies of Tragopogon dubius root fractions: antioxidant and antiproliferative 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