{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,8,21]],"date-time":"2025-08-21T16:37:37Z","timestamp":1755794257342,"version":"3.44.0"},"reference-count":61,"publisher":"FapUNIFESP (SciELO)","license":[{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"LPPM","award":["452\/UN27.21\/PN\/2020"],"award-info":[{"award-number":["452\/UN27.21\/PN\/2020"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Braz. J. Biol."],"abstract":"<jats:p>Abstract Various plants can be used as safe and effective antifertility agents, one of which is cashew (Anacardium occidentale L.). Cashew nut shells contain phenolic compounds Cashew Nut Shell Liquid (CNSL) or lacquer oil, which are effective antifertility agents in female rats, but there has been no research on male rats. The purpose of the study of antifertility agents of cashew nut shell extract in male rats includes analysis of the body weight, histological structure of the testes, testosterone levels, and assessing the microscopic quality of spermatozoa. Experimental research in the laboratory with a Completely Randomized Design. The test animals were Rattus norvegicus male Wistar strain aged two months, weighing 170-190 gr, totaling 24 divided into four treatment groups, each with six replications. The treatments given were 0.5% CMCNa (Control\/T0), cashew nut shell extract 250 mg\/kg body weight (Treatment 1\/T1), 500 mg\/kg body weight (T2), and 750 mg\/kg body weight (T3). The treatment period was 25 days and every seven days body weight was measured. Testicular preparations were made using the paraffin method and Hematoxylin Eosin staining. Testosterone levels were tested using the Enzyme-linked Immunosorbent Assay method. Microscopic spermatozoa quality tests included motility, morphology, and number of spermatozoa. Analysis of the histological structure of the testes was analyzed descriptively qualitatively by comparing between treatments. One-way ANOVA at the 5% level was used to measure rat body weight, testosterone levels, motility, morphology and number of spermatozoa. The results showed that cashew nut shell extract had no effect on rat body weight (p&gt;0.001). Repeated one-way measures to find out if the rat's mean body weight varied amongst the four treatments, an ANOVA was conducted. The rat's body weight did not differ statistically significantly between the treatments, according to an ANOVA (F statistic is 0.457 and the corresponding p value is 0.649). The rats body weight did not differ statistically significantly across the four treatments, according to the results of Bonferroni's test for multiple comparisons. The extract affected the histology of the seminiferous tubules in T3, namely atrophy, irregular shape, vacuolization and membrane fluidity. Between the interstitial tissue and the seminiferous tubules of the testes of rats in groups T1, T2 and T3 there was a gap or empty space called the phenomenon of tissue stretching (compliance). The extract significantly affected testosterone levels (p&lt;0.001) and microscopic quality of spermatozoa, namely motility (p&lt;0.001), morphology (p&lt;0.001) and number of spermatozoa (p&lt;0.001) which decreased with increasing extract dose. The results of the study concluded that the extract did not significantly affect the weight of rat, there were differences in the structure of the seminiferous tubules in T3, the extract significantly affected testosterone hormone levels and microscopic quality of spermatozoa (motility, normal morphology and number of spermatozoa) which decreased with increasing extract dose. The results of this study can be a reference for natural antifertility compounds.<\/jats:p>","DOI":"10.1590\/1519-6984.293201","type":"journal-article","created":{"date-parts":[[2025,8,15]],"date-time":"2025-08-15T15:04:31Z","timestamp":1755270271000},"source":"Crossref","is-referenced-by-count":0,"title":["Enhancing the antifertility performance by cashew nut shell extract (Anacardium occidentale L.) on Rattus norvegicus Berkenhout 1769"],"prefix":"10.1590","volume":"85","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2456-5826","authenticated-orcid":false,"given":"H.","family":"Harlita","sequence":"first","affiliation":[{"name":"Universitas Sebelas Maret,  Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2000-671X","authenticated-orcid":false,"given":"M.","family":"Indrowati","sequence":"additional","affiliation":[{"name":"Universitas Sebelas Maret,  Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2477-3791","authenticated-orcid":false,"given":"D. P.","family":"Sari","sequence":"additional","affiliation":[{"name":"Universitas Sebelas Maret,  Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-9561-1259","authenticated-orcid":false,"given":"W.","family":"Nugroho","sequence":"additional","affiliation":[{"name":"Universitas Sebelas Maret,  Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","published-online":{"date-parts":[[2025]]},"reference":[{"issue":"1","key":"ref1","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1186\/s12958-016-0177-6","article-title":"Sperm motility and morphology changes in rats exposed to cadmium and diazinon","volume":"14","author":"ADAMKOVICOVA M.","year":"2016","journal-title":"Reproductive Biology and Endocrinology"},{"issue":"1","key":"ref2","first-page":"43","article-title":"Alcohol consumption and its effect on testicular structure and on sperm count and motility in parent mice and their offspring","volume":"12","author":"ALBADRI C.T.","year":"2013","journal-title":"International Medical Journal Malaysia"},{"key":"ref3","series-title":"Hasil sensus penduduk (sp2020) pada september 2020 mencatat jumlah penduduk sebesar 270,20 juta jiwa","year":"2021"},{"issue":"4","key":"ref4","article-title":"Development of assays for the characterization of sperm motility parameters, viability, and membrane integrity in the epididymis and vas deferens of the greater rhea (Rhea americana)","volume":"20","author":"BEZERRA L.G.P.","year":"2023","journal-title":"Animal Reproduction"},{"issue":"2","key":"ref5","doi-asserted-by":"crossref","first-page":"68","DOI":"10.3390\/suschem5020006","article-title":"Isolation of cardanol fractions from cashew nutshell liquid (cnsl): a sustainable approach","volume":"5","author":"BHATIA B.","year":"2024","journal-title":"Sustainable Chemistry"},{"key":"ref6","series-title":"Biology.","author":"CHAMPBELL 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Andrology"},{"issue":"2","key":"ref10","first-page":"77","article-title":"Effects of aqueous extract of Anacardium occidentale (cashew) leaf on pregnancy outcome of wistar rats","volume":"3","author":"DARE S.S.","year":"2011","journal-title":"International Journal of Animal and Veterinary Advances"},{"issue":"2","key":"ref11","first-page":"189","article-title":"Antifertility activity of Oroxylum indicum vent. stem bark on female wistar rats","volume":"23","author":"DAS B.","year":"2024","journal-title":"Indian Journal of Traditional Knowledge"},{"issue":"7","key":"ref12","doi-asserted-by":"crossref","first-page":"1517","DOI":"10.18311\/jnr\/2024\/35409","article-title":"Evaluation of antifertility potential of Entada rheedii spreng seed kernel extract in female wistar rats","volume":"24","author":"DAWANE J.","year":"2024","journal-title":"Journal of Natural Remedies"},{"issue":"2","key":"ref13","doi-asserted-by":"crossref","first-page":"87","DOI":"10.18869\/nrip.irj.15.2.87","article-title":"Male infertility and its impact on women\u2019s sexual behaviors: need attention to psychological problem as a psychological rehabilitation","volume":"15","author":"GHAVI F.","year":"2017","journal-title":"Iranian Rehabilitation Journal"},{"key":"ref14","series-title":"Rat histopathology: a glossary for use in toxicity and carcinogenicity studies.","author":"GREAVES P.","year":"1984"},{"issue":"3","key":"ref15","first-page":"1","article-title":"The effect of noise to morphology of rats (mus musculus) spermatozoa","volume":"5","author":"HALIMAH K.","year":"2020","journal-title":"Public Health Perspective Journal"},{"key":"ref16","series-title":"Guyton and hall textbook of medical physiology.","author":"HALL J.E.","year":"1991"},{"key":"ref17","author":"HANDAYANI N.","year":"2024"},{"key":"ref18","first-page":"2","article-title":"Pengaruh ekstrak kulit biji mete (anacardium occidentale l) terhadap siklus estrus tikus albino (rattus norvegicus berkehout 1769)","volume":"1","author":"HARLITA H.","year":"2019","journal-title":"Seminar Nasional Pendidikan Sains"},{"issue":"2","key":"ref19","doi-asserted-by":"crossref","first-page":"1","DOI":"10.20961\/bioedukasi-uns.v8i2.3860","article-title":"Perubahan histologis uterus tikus putih (Rattus norvegicus) galur wistar: aktifitas antifertilitas ekstrak kulit biji mete (Anacardium occidentale L.)","volume":"8","author":"HARLITA R.M.P.","year":"2015","journal-title":"Bioedukasi: Jurnal Pendidikan Biologi"},{"issue":"2","key":"ref20","doi-asserted-by":"crossref","first-page":"89","DOI":"10.3923\/pjbs.2016.89.94","article-title":"Acute toxicity of cashew nut shell extract (Anacardium occidentale L.) in albino rat (Rattus norvegicus Berkenhout 1769)","volume":"19","author":"HARLITA N.H.","year":"2016","journal-title":"Pakistan Journal of Biological 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Medicine"},{"issue":"1","key":"ref24","doi-asserted-by":"crossref","first-page":"26","DOI":"10.2478\/intox-2019-0004","article-title":"Acute and subacute toxicity of ammi visnaga on rats","volume":"12","author":"KORIEM K.M.M.","year":"2019","journal-title":"Interdisciplinary Toxicology"},{"issue":"1","key":"ref25","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1016\/j.jfda.2014.10.001","article-title":"Effects of indium chloride exposure on sperm morphology and DNA integrity in rats","volume":"23","author":"LEE K.H.","year":"2015","journal-title":"Journal of Food and Drug Analysis"},{"key":"ref26","series-title":"Plant growth and development.","author":"LEOPOLD A.C.","year":"1983","edition":"2nd"},{"issue":"1","key":"ref27","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1186\/s12610-023-00204-z","article-title":"Male contraception: narrative review of ongoing research","volume":"33","author":"LOUWAGIE E.J.","year":"2023","journal-title":"Basic and Clinical Andrology"},{"issue":"2","key":"ref28","doi-asserted-by":"crossref","first-page":"105","DOI":"10.14421\/biomedich.2020.92.105-107","article-title":"A simple technique for rapid assessment of rat (Rattus norvegicus) sperm motility","volume":"9","author":"LUTHFI M.J.","year":"2021","journal-title":"Biology, Medicine, & Natural Product Chemistry"},{"issue":"3","key":"ref29","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1016\/j.chmed.2020.06.001","article-title":"Phytochemical analysis and antifertility potential of cynodon dactylon in female wistar rats: a herbal approach towards contraception","volume":"12","author":"MALPANI A.","year":"2020","journal-title":"Chinese Herbal Medicines"},{"key":"ref30","series-title":"Fish pathology section.","author":"MEYERS T.R.","year":"2000"},{"issue":"5","key":"ref31","doi-asserted-by":"crossref","first-page":"598","DOI":"10.1002\/j.1939-4640.2002.tb02296.x","article-title":"Androgen-binding protein and reproduction: where do we stand?","volume":"23","author":"MUNELL F.","year":"2002","journal-title":"Journal of Andrology"},{"key":"ref32","series-title":"Physiology, testosterone.","author":"NASSAR G.N.","year":"2018"},{"key":"ref33","series-title":"Endocrinology of the male reproductive system and spermatogenesis.","author":"O\u2019DONNELL L.","year":"2015"},{"key":"ref34","doi-asserted-by":"crossref","DOI":"10.1016\/j.jri.2023.103923","article-title":"Buchholzia coriacea seed induce antifertility by interfering with steroidogenic enzymes and inflammatory cytokines in rat testis","volume":"157","author":"OBEMBE O.O.","year":"2023","journal-title":"Journal of Reproductive Immunology"},{"issue":"1","key":"ref35","first-page":"1","article-title":"A literature review on contraceptive practices, barriers and measures to improve use among postpartum women","volume":"4","author":"OCHALA E.","year":"2021","journal-title":"African Journal of Health, Nursing and Midwifery"},{"issue":"2","key":"ref36","doi-asserted-by":"crossref","first-page":"101","DOI":"10.32725\/jab.2009.010","article-title":"Glutathione, glutathione-dependent enzymes and antioxidant status in gastric carcinoma patients","volume":"7","author":"PASUPATHI P.","year":"2009","journal-title":"Journal of Applied Biomedicine"},{"issue":"3","key":"ref37","first-page":"6","article-title":"Review of extraction techniques extraction methods: microwave, ultrasonic, pressurized fluid, soxhlet extraction, Etc","volume":"6","author":"PATEL K.","year":"2019","journal-title":"International Journal of Advanced Research in Chemical Science"},{"issue":"3","key":"ref38","first-page":"147","article-title":"Apoptosis pada spermatogenesis","volume":"22","author":"PRAMANINGTYAS M.D.","year":"2022","journal-title":"Jurnal Kedokteran Syiah Kuala"},{"key":"ref39","first-page":"485","article-title":"In vivo anti-fertility and anti-implantation activity of anacardic acid methyl ester in rats","volume":"3","author":"PRASAD F.","year":"2007","journal-title":"Pharmacologyonline"},{"issue":"1","key":"ref40","first-page":"22","article-title":"Effects of Gynura procumbens on sperm quality and testosterone level in streptozotocin-induced type 1 diabetic rats","volume":"8","author":"PUSPARANEE H.","year":"2016","journal-title":"International Journal of Pharmacognosy and Phytochemical Research"},{"key":"ref41","series-title":"Industrial Reproductive Toxicology (IRDG) Computer Assisted Sperm Analysis (CASA) group rat sperm morphological assessment edition.","year":"2000"},{"issue":"19","key":"ref42","doi-asserted-by":"crossref","DOI":"10.1016\/j.heliyon.2024.e38591","article-title":"Energy metabolism and spermatogenesis","volume":"10","author":"ROTIMI D.E.","year":"2024","journal-title":"Heliyon"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1007\/s12595-024-00516-6","article-title":"Assessment of oxidative liver injury caused by cashew nut shell liquid (cnsl) via interacting with bcl2 gene in male wistar rats and basic in-silico approaches","volume":"77","author":"SAHOO A.","year":"2024","journal-title":"Proceedings of the Zoological Society"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1007\/s12595-024-00516-6","article-title":"Assessment of oxidative liver injury caused by cashew nut shell liquid (cnsl) via interacting with bcl2 gene in male wistar rats and basic in-silico approaches","volume":"77","author":"SAHOO A.","year":"2024","journal-title":"Proceedings of the Zoological Society"},{"issue":"3","key":"ref45","doi-asserted-by":"crossref","first-page":"594","DOI":"10.1158\/1535-7163.MCT-09-0978","article-title":"Anacardic acid inhibits estrogen receptor \u03b1-DNA binding and reduces target gene transcription and breast cancer cell proliferation","volume":"9","author":"SCHULTZ D.J.","year":"2010","journal-title":"Molecular Cancer Therapeutics"},{"issue":"3","key":"ref46","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1016\/0890-6238(96)00028-7","article-title":"Methods for assessing sperm motility, morphology, and counts in the rat, rabbit, and dog: a consensus report","volume":"10","author":"SEED J.","year":"1996","journal-title":"Reproductive Toxicology (Elmsford, N.Y.)"},{"issue":"2","key":"ref47","first-page":"71","article-title":"Isolasi cashew nut shell liquid dari kulit biji jambu mete (anacardium occidentale l) dan kajian beberapa sifat fisiko-kimianya","volume":"2","author":"SIMPEN I.","year":"2018","journal-title":"Jurnal Kimia"},{"key":"ref48","first-page":"S50","article-title":"Toxicity evaluation of sappan wood extract in rats","volume":"93","author":"SIREERATAWONG S.","year":"2010","journal-title":"Journal of the Medical Association of Thailand"},{"key":"ref49","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1016\/j.semcdb.2014.02.012","article-title":"The regulation of spermatogenesis by androgens","volume":"30","author":"SMITH L.B.","year":"2014","journal-title":"Seminars in Cell & Developmental Biology"},{"issue":"3","key":"ref50","first-page":"125","article-title":"Seleksi spermatozoa domba garut dengan metode sentrifugasi gradien densitas percoll","volume":"10","author":"SUJOKO H.","year":"2019","journal-title":"Jurnal Veteriner"},{"issue":"2","key":"ref51","doi-asserted-by":"crossref","first-page":"52","DOI":"10.47213\/bp.v2i2.31","article-title":"Hubungan testosteron dengan motilitas spermatozoa tikus putih jantan (Ratus norwegicus) setelah diberi senyawa aktif daun beluntas untuk penyusun buku antifertilitas","volume":"2","author":"SUSETYARINI E.","year":"2019","journal-title":"Belantika Pendidikan"},{"key":"ref52","series-title":"Spermatologi.","author":"SUSILAWATI T.","year":"2011"},{"issue":"2","key":"ref53","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1002\/j.1939-4640.2001.tb02176.x","article-title":"Sperm structural and motility changes during aging in the brown norway rat","volume":"22","author":"SYNTIN P.","year":"2001","journal-title":"Journal of Andrology"},{"issue":"1","key":"ref54","first-page":"1","article-title":"Different of hypothalamic-pituitary-gonadal axis in male and female","volume":"6","author":"TAMMASSE I.F.U.","year":"2023","journal-title":"Reproductive Medicine International"},{"issue":"1","key":"ref55","first-page":"49","article-title":"Cut-off values for normal sperm morphology and toxicology for automated analysis of rat sperm morphology and morphometry","volume":"93","author":"VAN DER HORST G.","year":"2018","journal-title":"Biotechnic & Histochemistry"},{"issue":"6","key":"ref56","first-page":"732","article-title":"Short-term storage of rat sperm in the presence of various extenders","volume":"52","author":"VARISLI O.","year":"2013","journal-title":"Journal of the American Association for Laboratory Animal Science; JAALAS"},{"issue":"4","key":"ref57","doi-asserted-by":"crossref","first-page":"554","DOI":"10.1002\/j.1939-4640.2004.tb02826.x","article-title":"Sperm morphology and preparation method affect bovine embryonic development","volume":"25","author":"WALTERS A.H.","year":"2004","journal-title":"Journal of Andrology"},{"key":"ref58","series-title":"The examination and processing of human semen.","year":"2021","edition":"6th"},{"issue":"1","key":"ref59","first-page":"115","article-title":"The effect of eucalyptus globulus hydroalcoholic extract on lh, fsh and testosterone concentrations and sperm morphology","volume":"78","author":"WIDJAJA G.","year":"2023","journal-title":"Archives of Razi Institute"},{"issue":"1","key":"ref60","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1186\/s12894-020-00674-7","article-title":"Circulating sex hormone levels in relation to male sperm quality","volume":"20","author":"ZHAO W.","year":"2020","journal-title":"BMC 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