{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,27]],"date-time":"2025-09-27T00:10:25Z","timestamp":1758931825044,"version":"3.44.0"},"reference-count":30,"publisher":"FapUNIFESP (SciELO)","license":[{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Braz. J. Biol."],"abstract":"<jats:p>Abstract The presence of antibiotic residues in bovine meat is a significant public health concern, particularly in regions with limited regulation of veterinary drug usage. Tetracyclines, commonly used in cattle for therapeutic and prophylactic purposes, can persist in edible tissues if withdrawal periods are not properly observed. This study aimed to qualitatively determine the presence of tetracycline residues in bovine meat from the municipal slaughterhouse of Santa Rosa Canton, El Oro Province, Ecuador. A total of 74 meat samples were collected from the neck muscle of slaughtered cattle. The samples were analyzed using the SmarKit Rapid Test, a qualitative immunoassay designed to detect oxytetracycline, tetracycline, and chlortetracycline residues. Data regarding the age, sex, and origin of the animals were recorded. Statistical analysis was performed using SAS v.9.4, applying descriptive statistics and a Chi-square test to evaluate associations between residue presence and animal variables. Of the 74 samples analyzed, 24 (32.4%) tested positive for tetracycline residues. A significant association was observed between the age of the animals and the presence of residues (P &lt; 0.05), with younger cattle showing higher prevalence rates. No significant correlations were found between residue presence and the sex or origin of the animals. The results highlight gaps in adherence to withdrawal periods and the potential risks to public health from consuming contaminated meat. In conclusions, this study underscores the need for improved veterinary oversight and education for producers on the responsible use of antibiotics in cattle. Additionally, implementing routine screening methods like SmarKit in slaughterhouses can enhance food safety monitoring. Addressing these issues is critical for reducing public health risks and promoting sustainable livestock practices in Ecuador.<\/jats:p>","DOI":"10.1590\/1519-6984.295997","type":"journal-article","created":{"date-parts":[[2025,9,26]],"date-time":"2025-09-26T15:01:37Z","timestamp":1758898897000},"source":"Crossref","is-referenced-by-count":0,"title":["Qualitative determination of tetracycline residues in bovine meat at the level of the municipal slaughterhouse of Santa Rosa Canton, Oro province, Ecuador"],"prefix":"10.1590","volume":"85","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-4381-7776","authenticated-orcid":false,"given":"M. V. F.","family":"Blacio","sequence":"first","affiliation":[{"name":"Escuela Superior Polit\u00e9cnica de Chimborazo,  Ecuador"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-4053-5723","authenticated-orcid":false,"given":"M. G. M.","family":"Mora","sequence":"additional","affiliation":[{"name":"Escuela Superior Polit\u00e9cnica de Chimborazo,  Ecuador"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-4934-5822","authenticated-orcid":false,"given":"N. S. T.","family":"Santill\u00e1n","sequence":"additional","affiliation":[{"name":"Escuela Superior Polit\u00e9cnica de Chimborazo,  Ecuador"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9168-154X","authenticated-orcid":false,"given":"J.","family":"Llivi-Marcatoma","sequence":"additional","affiliation":[{"name":"Escuela Superior Polit\u00e9cnica de Chimborazo,  Ecuador"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N. E. A.","family":"Rivera","sequence":"additional","affiliation":[{"name":"Independent Researcher,  Ecuador"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-7713-3561","authenticated-orcid":false,"given":"H. J. V.","family":"Caiminagua","sequence":"additional","affiliation":[{"name":"Independent Researcher,  Ecuador"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5056-9180","authenticated-orcid":false,"given":"L. Cabezas","family":"Andrade","sequence":"additional","affiliation":[{"name":"Independent Researcher,  Ecuador"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8699-0655","authenticated-orcid":false,"given":"S. A. G.","family":"Rivera","sequence":"additional","affiliation":[{"name":"Universidad Aut\u00f3noma de Barcelona,  Espa\u00f1a"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","published-online":{"date-parts":[[2025]]},"reference":[{"issue":"39","key":"ref1","doi-asserted-by":"crossref","first-page":"143","DOI":"10.17151\/luaz.2014.39.9","article-title":"Determinaci\u00f3n de residuos de oxitetraciclina en muestras de carne bovina","volume":"39","author":"ACOSTA AGUDELO S.","year":"2014","journal-title":"Luna Azul"},{"issue":"4","key":"ref2","doi-asserted-by":"crossref","first-page":"306","DOI":"10.1016\/j.foodcont.2005.10.013","article-title":"Antimicrobial resistance of Salmonella Spp. and Escherichia coli isolated from table eggs","volume":"18","author":"ADESIYUN A.","year":"2007","journal-title":"Food Control"},{"key":"ref3","series-title":"Elaboraci\u00f3n de un plan de negocios para la producci\u00f3n de carne de ganado vacuno en las finca Santa Luc\u00eda y San Jorge ubicadas en la provincia de Imbabura","author":"CABEZAS M.J.A.","year":"2010"},{"issue":"1","key":"ref4","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1186\/s40104-016-0127-3","article-title":"Antimicrobial usage and resistance in beef production","volume":"7","author":"CAMERON A.","year":"2016","journal-title":"Journal of Animal Science and Biotechnology"},{"key":"ref5","first-page":"S13","article-title":"Antibiotic residues in food: the African scenario","volume":"61","author":"DARWISH W.S.","year":"2013","journal-title":"The Japanese Journal of Veterinary Research"},{"issue":"5","key":"ref6","doi-asserted-by":"crossref","first-page":"594","DOI":"10.2105\/AJPH.47.5.594","article-title":"Use of antibiotics in the preservation of meats and other food products","volume":"47","author":"DEATHERAGE F.E.","year":"1957","journal-title":"American Journal of Public Health and the Nation\u2019s Health"},{"key":"ref7","series-title":"Encuesta de superficie y producci\u00f3n agropecuaria continua, 2019.","year":"2022"},{"key":"ref8","series-title":"Antimicrobial resistance and the united nations sustainable development cooperation framework: guidance for united nations country teams.","year":"2021"},{"key":"ref9","series-title":"OECD-FAO Agricultural Outlook 2021-2030.","year":"2021"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"1881","DOI":"10.3389\/fmicb.2016.01881","article-title":"Antibiotic resistance in the food chain: a developing country-perspective","volume":"7","author":"FOUNOU L.L.","year":"2016","journal-title":"Frontiers in Microbiology"},{"issue":"20","key":"ref11","first-page":"67","article-title":"Determinaci\u00f3n de residuos de tetraciclinas en muestras de carne bovina destinadas al consumo humano","author":"G\u00c1LVEZ F.L.A.","year":"2018","journal-title":"La T\u00e9cnica"},{"issue":"March","key":"ref12","article-title":"A one health perspective on dairy production and dairy food safety","volume":"7","author":"GARCIA S.N.","year":"2019","journal-title":"One Health"},{"issue":"2","key":"ref13","doi-asserted-by":"crossref","DOI":"10.1016\/j.animal.2020.100123","article-title":"Review: mitigating the risks posed by intensification in livestock production \u2013 the examples of antimicrobial resistance and zoonoses","volume":"15","author":"GILBERT W.","year":"2021","journal-title":"Animal"},{"key":"ref14","series-title":"An\u00e1lisis de la situaci\u00f3n del uso de medicamentos (antibi\u00f3ticos y antipar\u00e1sitarios) en las unidades productivas de los centros de acopio y enfriamiento de leche.","author":"GUATEMAL C.","year":"2012"},{"issue":"1","key":"ref15","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1186\/1744-8603-9-48","article-title":"Restrictions on antimicrobial use in food animal production : an international regulatory and economic survey","volume":"9","author":"MARON D.","year":"2013","journal-title":"Globalization and Health"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/j.anifeedsci.2017.05.008","article-title":"Phytogenic Feed Additives as Potential Gut Contractility Modifiers: a Review","volume":"230","author":"MENDEL M.","year":"2017","journal-title":"Animal Feed Science and Technology"},{"key":"ref17","series-title":"Tetracyclines use in animals","author":"MERCER M.A.","year":"2022"},{"issue":"2","key":"ref18","doi-asserted-by":"crossref","first-page":"97","DOI":"10.4142\/jvs.2001.2.2.97","article-title":"Tetracycline residue levels in cattle meat from Nairobi salughter house in Kenya","volume":"2","author":"MURIUKI F.K.","year":"2001","journal-title":"Journal of Veterinary Science"},{"issue":"2","key":"ref19","doi-asserted-by":"crossref","first-page":"93","DOI":"10.4314\/nvj.v31i2.68952","article-title":"Oxytetracycline residues in edible tissues of cattle slaughtered in Akure, Nigeria","volume":"31","author":"OLATOYE I.","year":"2011","journal-title":"Nigerian Veterinary Journal"},{"key":"ref20","series-title":"Diagn\u00f3stico y desarrollo del plan de manejo ambiental para el camal de otavalo","author":"PAREDES M.G.G.","year":"2011"},{"issue":"6","key":"ref21","doi-asserted-by":"crossref","DOI":"10.1136\/bmjgh-2019-002104","article-title":"Tackling antimicrobial resistance in low-income and middle-income countries","volume":"4","author":"POKHAREL S.","year":"2019","journal-title":"BMJ Global Health"},{"issue":"4","key":"ref22","doi-asserted-by":"crossref","first-page":"536","DOI":"10.1007\/s43441-022-00396-y","article-title":"Regulation of drug prescribing information in Latin America and the Caribbean","volume":"56","author":"RAM\u00cdREZ-TELLES M.","year":"2022","journal-title":"Therapeutic Innovation & Regulatory Science"},{"issue":"1","key":"ref23","doi-asserted-by":"crossref","first-page":"55","DOI":"10.17163\/lgr.n15.2012.05","article-title":"Caracterizaci\u00f3n de sistemas de producci\u00f3n lechera de Ecuador","volume":"15","author":"REQUELME N.","year":"2012","journal-title":"La Granja"},{"issue":"4","key":"ref24","article-title":"Genome wide analysis of citrus sinensis heat shock proteins","volume":"18","author":"SHAFQAT W.","year":"2020","journal-title":"Iranian Journal of Biotechnology"},{"issue":"8","key":"ref25","doi-asserted-by":"crossref","first-page":"1303","DOI":"10.3390\/antibiotics12081303","article-title":"Regulations on the use of antibiotics in livestock production in South America: a comparative literature analysis","volume":"12","author":"SILVA R.A.","year":"2023","journal-title":"Antibiotics"},{"key":"ref26","first-page":"275","author":"SRIYENI Y.","year":"2022"},{"key":"ref27","series-title":"Determinaci\u00f3n de antibi\u00f3ticos en carne vacuna y porcina, proveniente del norte antioque\u00f1o en la planta frigocolanta ubicada en el municipio de Santa Rosa de Osos","author":"V\u00c9LEZ C.","year":"2013"},{"key":"ref28","series-title":"Deteccion de la presencia de antibioticos en canales bovinas faenadas en el camal municipal de la ciudad de Azogues mediante la prueba microbiana premi\u00ae-test.","author":"VILLA M.","year":"2016"},{"issue":"October","key":"ref29","doi-asserted-by":"crossref","first-page":"128","DOI":"10.3389\/fsufs.2020.00128","article-title":"Plant-based meats, human health, and climate change","volume":"4","author":"VLIET S.V.","year":"2020","journal-title":"Frontiers in Sustainable Food Systems"},{"issue":"4","key":"ref30","first-page":"1","article-title":"Antibiotic residues, antimicrobial resistance and intervention strategies of foodborne pathogens","volume":"13","author":"WU Y.","year":"2024","journal-title":"Antibiotics"}],"container-title":["Brazilian Journal of Biology"],"original-title":["Determina\u00e7\u00e3o qualitativa de res\u00edduos de tetraciclina em carne bovina no matadouro municipal do Cant\u00e3o de Santa Rosa, prov\u00edncia de El Oro, Equador"],"link":[{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_pdf&pid=S1519-69842025000100326&tlng=en","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,26]],"date-time":"2025-09-26T15:01:41Z","timestamp":1758898901000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_arttext&pid=S1519-69842025000100326&tlng=en"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025]]},"references-count":30,"alternative-id":["S1519-69842025000100326"],"URL":"https:\/\/doi.org\/10.1590\/1519-6984.295997","relation":{},"ISSN":["1678-4375","1519-6984"],"issn-type":[{"value":"1678-4375","type":"electronic"},{"value":"1519-6984","type":"print"}],"subject":[],"published":{"date-parts":[[2025]]},"article-number":"e295997"}}