{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T12:09:58Z","timestamp":1778501398099,"version":"3.51.4"},"reference-count":52,"publisher":"FapUNIFESP (SciELO)","license":[{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"University Brawijaya","award":["102 \/UN10\/KS\/2025"],"award-info":[{"award-number":["102 \/UN10\/KS\/2025"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Braz. J. Biol."],"abstract":"<jats:p>Abstract Lipoxygenase (LOX)-derived eicosanoids (12-\/15-HETE) and Molt-Inhibiting Hormone (MIH) are key mediators of inflammation and stress physiology in Litopenaeus vannamei during bacterial challenge. Squid ink (Loligo sp.) contains bioactives (betaine, choline, cinnamic acid) with potential immunomodulatory activity. This study aim to evaluate whether dietary squid ink extract modulates MIH responses, LOX-related metabolites, and survival of L. vannamei challenged with Vibrio harveyi under different salinities. Squid ink was methanol-macerated and evaporated to paste; 8 g paste was dissolved in 1 L water and spray-coated onto per kg commercial feed (fed at 5% biomass\/day, 4 times\/day). Shrimp were reared in 12 aquaria at 24, 27, and 30 ppt, with an infected positive control at 33 ppt, then challenged by immersion with V. harveyi (10^6 CFU\/mL). MIH was identified by PCR, 12-HETE\/15-HETE were quantified by LC\u2013MS, and ligand\u2013protein interactions were explored by interaction in silico analysis. Squid ink-related treatments reduced 12-HETE from 2.0 \u00b1 0.3 to 0.2\u20130.5 nmol\/mg (ANOVA F = 6.929; p = 0.006), whereas 15-HETE remained near the observed range (5.5\u20136.0 nmol\/mg). MIH amplicons (250\u2013283 bp) showed the strongest band intensity at 30 ppt. Survival improved in supplemented groups (80\u201390%) relative to the positive control (73.33 \u00b1 5.77%), with the highest survival at 30 ppt (90 \u00b1 10%) (post hoc, p &lt; 0.05). Dietary squid ink extract attenuated the pro-inflammatory 12-LOX pathway and improved post-challenge survival, with responses depending on salinity, supporting squid ink as a natural immunostimulant candidate for shrimp aquaculture.<\/jats:p>","DOI":"10.1590\/1519-6984.301167","type":"journal-article","created":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T11:27:13Z","timestamp":1778498833000},"source":"Crossref","is-referenced-by-count":0,"title":["Immunomodulatory effect of squid ink extract on lipoxygenase pathways and MIH gene in Litopenaeus vannamei under different salinity condition"],"prefix":"10.1590","volume":"86","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2318-1698","authenticated-orcid":false,"given":"M.","family":"Fadjar","sequence":"first","affiliation":[{"name":"University of Brawijaya,  Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8601-8919","authenticated-orcid":false,"given":"S.","family":"Andayani","sequence":"additional","affiliation":[{"name":"University of Brawijaya,  Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8142-3192","authenticated-orcid":false,"given":"H.","family":"K\u00fchn","sequence":"additional","affiliation":[{"name":"Charit\u00e9-Universit\u00e4tsmedizin Berlin,  Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-9244-2950","authenticated-orcid":false,"given":"D.","family":"Aisyah","sequence":"additional","affiliation":[{"name":"University of Brawijaya,  Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5105-7600","authenticated-orcid":false,"given":"A. W.","family":"Ramadhani","sequence":"additional","affiliation":[{"name":"University of Brawijaya,  Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-4525-1431","authenticated-orcid":false,"given":"C.","family":"Herlina","sequence":"additional","affiliation":[{"name":"University of Brawijaya,  Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","published-online":{"date-parts":[[2026]]},"reference":[{"key":"ref1","series-title":"Vaname Shrimp (Litopenaeus vannamei) closed environment friendly system","author":"ADIWIJAYA D.","year":"2003"},{"issue":"3","key":"ref2","doi-asserted-by":"crossref","first-page":"150","DOI":"10.21776\/ub.rjls.2019.006.03.1","article-title":"Active compounds on squid (Loligo sp.) ink extract powder as immunostimulants candidate to against shrimp disease","volume":"6","author":"AFFANDI R.I.","year":"2019","journal-title":"Research Journal of Life Science"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1016\/j.jep.2017.08.008","article-title":"Review of anti-inflammatory, immune-modulatory and wound healing properties of molluscs","volume":"210","author":"AHMAD T.B.","year":"2018","journal-title":"Journal of Ethnopharmacology"},{"issue":"1","key":"ref4","doi-asserted-by":"crossref","first-page":"787","DOI":"10.1038\/s41598-019-57409-4","article-title":"MicroRNA and mRNA interactions coordinate the immune response in non-lethal heat stressed Litopenaeus vannamei against AHPND-causing Vibrio parahaemolyticus","volume":"10","author":"BOONCHUEN P.","year":"2020","journal-title":"Scientific Reports"},{"key":"ref5","series-title":"Evaluation of Belize Aquaculture, Ltd: A Superintensive Shrimp Aquaculture System.","author":"BOYD C.E.","year":"2002"},{"key":"ref6","doi-asserted-by":"crossref","DOI":"10.3389\/fncel.2023.1277268","article-title":"12\/15-lipoxygenase inhibition attenuates neuroinflammation by suppressing inflammasomes","volume":"17","author":"CAKIR-AKTAS C.","year":"2023","journal-title":"Frontiers in Cellular Neuroscience"},{"issue":"20","key":"ref7","first-page":"2786","article-title":"Lipoxygenase and carotenoids: a co-oxidation story","volume":"12","author":"CHEDEA V.S.","year":"2013","journal-title":"African Journal of Biotechnology"},{"issue":"7","key":"ref8","article-title":"Transcriptome and molecular pathway analysis of the hepatopancreas in the Pacific white shrimp Litopenaeus vannamei under chronic low-salinity stress","volume":"10","author":"CHEN K.","year":"2015","journal-title":"PLoS One"},{"issue":"6","key":"ref9","doi-asserted-by":"crossref","first-page":"654","DOI":"10.1152\/ajpendo.00373.2011","article-title":"2\/15-Lipoxygenase signaling in the endoplasmic reticulum stress response","volume":"302","author":"COLE B.K.","year":"2012","journal-title":"American Journal of Physiology. 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salinidade"],"link":[{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_pdf&pid=S1519-69842026000100292&tlng=en","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T11:27:21Z","timestamp":1778498841000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_arttext&pid=S1519-69842026000100292&tlng=en"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026]]},"references-count":52,"alternative-id":["S1519-69842026000100292"],"URL":"https:\/\/doi.org\/10.1590\/1519-6984.301167","relation":{},"ISSN":["1678-4375","1519-6984"],"issn-type":[{"value":"1678-4375","type":"electronic"},{"value":"1519-6984","type":"print"}],"subject":[],"published":{"date-parts":[[2026]]},"article-number":"e301167"}}