{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T12:08:13Z","timestamp":1776427693130,"version":"3.51.2"},"reference-count":36,"publisher":"FapUNIFESP (SciELO)","license":[{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordination for the Improvement of Higher Education Personnel","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Braz. J. Biol."],"abstract":"<jats:p>Abstract Due to the increasing demand for sustainable alternatives to antibiotics in aquaculture, the selection of indigenous probiotics with multi-functional properties is essential for improving fish health and food safety. This research aimed to evaluate the probiotic potential and Aflatoxin B1 (AFB1) adsorption capacity of the yeast strain S. cerevisiae A8L3, isolated from a fish farm environment. The isolate, belonging to the NUEPPA culture collection, was subjected to in vitro assays to determine its autoaggregation and co-aggregation abilities, antimicrobial activity against pathogens, and tolerance to simulated stomach (pH 2.0) and intestinal (pH 7.0) conditions of Nile tilapia (Oreochromis niloticus). Results indicated that S. cerevisiae A8L3 possesses robust aggregative properties and effective bacterial inhibition for all strains evaluated. Furthermore, the strain demonstrated high viability and the ability to multiply under simulated gastrointestinal conditions. In addition to these probiotic traits, A8L3 showed a significant capacity for AFB1 adsorption. These findings confirm that S. cerevisiae A8L3 meets the essential requirements for inclusion in fish diets. The strain demonstrates significant potential as a dual-action biological agent, capable of enhancing pathogen resistance and mitigating risks associated with mycotoxin-contaminated feed, thus offering a promising strategy for sustainable Nile tilapia farming.<\/jats:p>","DOI":"10.1590\/1519-6984.302330","type":"journal-article","created":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T11:33:55Z","timestamp":1776425635000},"source":"Crossref","is-referenced-by-count":0,"title":["Probiotic and adsorbent viability of aflatoxin B1 by the yeast Saccharomyces cerevisiae A8L3 isolated from fish farming environment"],"prefix":"10.1590","volume":"86","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8315-8804","authenticated-orcid":false,"given":"E. J. R.","family":"Santos","sequence":"first","affiliation":[{"name":"Universidade Federal do Piau\u00ed,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6559-3463","authenticated-orcid":false,"given":"J. F.","family":"Sousa-Junior","sequence":"additional","affiliation":[{"name":"Universidade Federal do Piau\u00ed,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4795-9254","authenticated-orcid":false,"given":"M. P. D.","family":"Lima","sequence":"additional","affiliation":[{"name":"Universidade Federal do Piau\u00ed,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4569-0995","authenticated-orcid":false,"given":"M. C. S.","family":"Muratori","sequence":"additional","affiliation":[{"name":"Universidade Federal do Piau\u00ed,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","published-online":{"date-parts":[[2026]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1186\/s12866-017-1000-z","article-title":"In vitro assessment of Pediococcus acidilactici Kp10 for its potential use in the food industry","volume":"17","author":"ABBASILIASI S.","year":"2017","journal-title":"BMC Microbiology"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1016\/j.rvsc.2017.01.016","article-title":"The advancement of probiotics research and its application in fish farming industries","volume":"115","author":"BANERJEE G.","year":"2017","journal-title":"Research in Veterinary Science"},{"key":"ref3","doi-asserted-by":"crossref","DOI":"10.1016\/j.foodres.2020.109505","article-title":"In vitro and in vivo capacity of yeast-based products to bind to a fl atoxins B 1 and M 1 in media and foodstu ff s: a systematic review and meta-analysis","volume":"137","author":"CAMPAGNOLLO F.B.","year":"2020","journal-title":"Food Research International"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"1093","DOI":"10.3389\/fmicb.2015.01093","article-title":"Potential probiotic yeasts isolated from the fish gut protect zebrafish (Danio rerio) from a Vibrio anguillarum challenge","volume":"6","author":"CARUFFO M.","year":"2015","journal-title":"Frontiers in Microbiology"},{"issue":"3","key":"ref5","first-page":"583","article-title":"Physiological responses of Nile tilapia (Oreochromis niloticus) under different environmental stressors","volume":"81","author":"FERREIRA G.S.","year":"2021","journal-title":"Brazilian Journal of Biology = Revista Brasileira de Biologia"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1016\/j.fm.2016.09.016","article-title":"Potential probiotic Pichia kudriavzevii strains and their ability to enhance folate content of traditional cereal-based African fermented food","volume":"62","author":"GREPPI A.","year":"2017","journal-title":"Food Microbiology"},{"issue":"3","key":"ref7","doi-asserted-by":"crossref","first-page":"116","DOI":"10.3390\/toxins10030116","article-title":"Promising detoxification strategies to mitigate mycotoxins in food and feed","volume":"10","author":"HASSAN Y.I.","year":"2018","journal-title":"Toxins"},{"issue":"8","key":"ref8","doi-asserted-by":"crossref","first-page":"506","DOI":"10.1038\/nrgastro.2014.66","article-title":"Expert consensus document: the international scientific association for probiotics and prebiotics consensus statement on the scope and appropriate use of the term probiotic","volume":"11","author":"HILL C.","year":"2014","journal-title":"Nature Reviews. Gastroenterology & Hepatology"},{"issue":"6","key":"ref9","doi-asserted-by":"crossref","first-page":"981","DOI":"10.1046\/j.1365-2672.2003.01915.x","article-title":"Adhesion and aggregation ability of probiotic strain Lactobacillus acidophilus M92","volume":"94","author":"KOS B.","year":"2003","journal-title":"Journal of Applied Microbiology"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1016\/j.tifs.2019.12.012","article-title":"Trends in Food Science & Technology Complicated interactions between bio-adsorbents and mycotoxins during mycotoxin adsorption: current research and future prospects","volume":"96","author":"LUO Y.","year":"2020","journal-title":"Trends in Food Science & Technology"},{"issue":"1","key":"ref11","doi-asserted-by":"crossref","first-page":"48","DOI":"10.3382\/ps.2010-00971","article-title":"Effect of low levels of aflatoxin B 1 on performance, biochemical parameters, and aflatoxin B 1 in broiler liver tissues in the presence of monensin and sodium bentonite","volume":"90","author":"MAGNOLI A.P.","year":"2011","journal-title":"Poultry Science"},{"issue":"2","key":"ref12","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1111\/jwas.12371","article-title":"Impact of mycotoxins on aquaculture fish species: a review","volume":"48","author":"MATEJOVA I.","year":"2017","journal-title":"Journal of the World Aquaculture Society"},{"issue":"4","key":"ref13","doi-asserted-by":"crossref","first-page":"487","DOI":"10.4034\/RBCS.2011.15.04.16","article-title":"Probi\u00f3ticos-revis\u00e3o da literatura","volume":"15","author":"NOGUEIRA J.C.R.","year":"2011","journal-title":"Revista Brasileira de Ci\u00eancias da Sa\u00fade"},{"issue":"3","key":"ref14","doi-asserted-by":"crossref","first-page":"797","DOI":"10.1111\/jam.12875","article-title":"Probiotic potentials of yeasts isolated from some cereal-based Nigerian traditional fermented food products","volume":"119","author":"OGUNREMI O.R.","year":"2015","journal-title":"Journal of Applied Microbiology"},{"issue":"16","key":"ref15","doi-asserted-by":"crossref","first-page":"6563","DOI":"10.1007\/s00253-015-6776-x","article-title":"Probiotic Saccharomyces cerevisiae strains as biotherapeutic tools: is there room for improvement","volume":"99","author":"PALMA M.L.","year":"2015","journal-title":"Applied Microbiology and Biotechnology"},{"issue":"1","key":"ref16","first-page":"25","article-title":"In vitro ochratoxin A adsorption by commercial yeast cell walls","volume":"37","author":"PEREYRA C.M.","year":"2015","journal-title":"Revista Brasileira de Medicina Veterin\u00e1ria"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"306","DOI":"10.1016\/j.foodres.2018.05.026","article-title":"The production of yeast cell wall using an agroindustrial waste in fl uences the wall thickness and is implicated on the a fl atoxin B 1 adsorption process","volume":"111","author":"PEREYRA C.M.","year":"2018","journal-title":"Food Research International"},{"issue":"7","key":"ref18","doi-asserted-by":"crossref","first-page":"805","DOI":"10.1002\/jobm.201400833","article-title":"Mycotoxins - prevention and decontamination by yeasts","volume":"55","author":"PFLIEGLER W.P.","year":"2015","journal-title":"Journal of Basic Microbiology"},{"issue":"0","key":"ref19","article-title":"Avalia\u00e7\u00e3o in vitro da adsor\u00e7\u00e3o de aflatoxina B1 por produtos comerciais utilizados na alimenta\u00e7\u00e3o animal","volume":"84","author":"PINHEIRO R.E.E.","year":"2017","journal-title":"Arquivos do Instituto Biol\u00f3gico"},{"issue":"3","key":"ref20","doi-asserted-by":"crossref","first-page":"862","DOI":"10.1590\/1678-4162-11280","article-title":"Saccharomyces cerevisiae as a probiotic agent and a possible aflatoxin B1 adsorbent in simulated fish intestinal tract conditions","volume":"72","author":"PINHEIRO R.E.E.","year":"2020","journal-title":"Arquivo Brasileiro de Medicina Veterin\u00e1ria e Zootecnia"},{"key":"ref21","series-title":"Fungi and food spoilage.","author":"PITT J.I.","year":"2009","edition":"3rd"},{"issue":"10","key":"ref22","first-page":"933","article-title":"Evaluation of Saccharomyces cerevisiae strains as probiotic agent with aflatoxin B1 adsorption ability for use in poultry feedstuffs","volume":"47","author":"PIZZOLITTO R.P.","year":"2012","journal-title":"Journal of Environmental Science and Health. 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Boulardii against human pathogens","volume":"50","author":"RAJKOWSKA K.","year":"2012","journal-title":"Food Technology and Biotechnology"},{"key":"ref28","series-title":"Aspectos gerais da fisiologia e estrutura do sistema digestivo dos peixes relacionados \u00e0 piscicultura.","volume":"53","author":"ROTTA M.A.","year":"2003"},{"issue":"4","key":"ref29","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1080\/02652039809374659","article-title":"A collaborative study of an HPLC method for determination of ochratoxin A in wheat using immunoaflinity column clean \u2010 up A collaborative study of an HPLC method for determination of ochratoxin A in wheat using immunoaflinity column clean-up","volume":"15","author":"SCUDAMORE K.A.","year":"1998","journal-title":"Food Additives and Contaminants"},{"issue":"4","key":"ref30","first-page":"967","article-title":"Probiotic supplementation in the diet of Nile tilapia: effects on growth performance, survival, and hematological parameters.","volume":"75","author":"SILVA B.C.","year":"2015","journal-title":"Brazilian Journal of Biology = Revista Brasileira de Biologia"},{"issue":"1-2","key":"ref31","first-page":"123","article-title":"Nowa metoda oceny antagonistycznego dzia\u0142ania bakterii kwasu mlekowego (LAB) na wybrane chorobotw\u00f3rcze bakterie wska\u017anikowe","volume":"50","author":"STRUS M.","year":"1998","journal-title":"Medycyna Do\u015bwiadczalna i Mikrobiologia"},{"issue":"12","key":"ref32","doi-asserted-by":"crossref","DOI":"10.33448\/rsd-v9i12.11031","article-title":"Desenvolvimento de bebidas probi\u00f3ticas n\u00e3o l\u00e1cteas adicionadas de Saccharomyces boulardii: situa\u00e7\u00e3o atual e perspectivas","volume":"9","author":"TERHAAG M.M.","year":"2020","journal-title":"Research, Social Development"},{"key":"ref33","series-title":"Micotoxinas no trigo: estrat\u00e9gias de manejo para minimizar a contamina\u00e7\u00e3o","volume":"21","author":"TIBOLA C.S.","year":"2020"},{"issue":"6","key":"ref34","doi-asserted-by":"crossref","first-page":"1512","DOI":"10.1093\/jaoac\/77.6.1512","article-title":"Multifunctional column coupled with liquid chromatography for determination of aflatoxins B1, B2, G1, and G2 in corn, almonds, brazil nuts, peanuts, and pistachio nuts: collaborative study","volume":"77","author":"TRUCKSESS M.W.","year":"1994","journal-title":"Journal of AOAC International"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3389\/fmicb.2021.647977","article-title":"Probiotic yeasts and Vibrio anguillarum Infection Modify the Microbiome of Zebrafish Larvae","volume":"12","author":"VARGAS O.","year":"2021","journal-title":"Frontiers in Microbiology"},{"issue":"8","key":"ref36","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/jfbc.12865","article-title":"Technological properties and probiotic potential of yeasts isolated from traditional low \u2010 salt fermented Chinese fish Suan yu","volume":"43","author":"ZENG X.","year":"2019","journal-title":"Journal of Food Biochemistry"}],"container-title":["Brazilian Journal of Biology"],"original-title":["Viabilidade probi\u00f3tica e adsorvente de aflatoxina B1 pela levedura Saccharomyces cerevisiae A8L3 isolada de ambiente de piscicultura"],"link":[{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_pdf&pid=S1519-69842026000100273&tlng=en","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T11:34:00Z","timestamp":1776425640000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_arttext&pid=S1519-69842026000100273&tlng=en"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026]]},"references-count":36,"alternative-id":["S1519-69842026000100273"],"URL":"https:\/\/doi.org\/10.1590\/1519-6984.302330","relation":{},"ISSN":["1678-4375","1519-6984"],"issn-type":[{"value":"1678-4375","type":"electronic"},{"value":"1519-6984","type":"print"}],"subject":[],"published":{"date-parts":[[2026]]},"article-number":"e302330"}}