{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T15:07:09Z","timestamp":1753888029737,"version":"3.41.2"},"reference-count":25,"publisher":"FapUNIFESP (SciELO)","license":[{"start":{"date-parts":[[2020,1,1]],"date-time":"2020-01-01T00:00:00Z","timestamp":1577836800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2020,1,1]],"date-time":"2020-01-01T00:00:00Z","timestamp":1577836800000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2020,1,1]],"date-time":"2020-01-01T00:00:00Z","timestamp":1577836800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. vasc. bras."],"abstract":"<jats:p>Abstract  Background Ischemia-reperfusion injury contributes to morbidity after revascularization procedures. Along with early reperfusion, tissue conditioning by alternating intervals of brief ischemia-reperfusion episodes is considered the best approach to limit tissue damage. Remote ischemic conditioning is conducted remotely, in tissues other than those under ischemia. Despite this, remote ischemic conditioning protection mechanisms are poorly understood, which can lead to misapplication.  Objectives To assess whether remote ischemic conditioning works in the heart and brain through enhancement of cells\u2019 antioxidant defenses and whether the response is sustained or temporary.  Methods Twenty-one male Wistar rats were assigned to three groups (n = 7): SHAM: same procedure as the other groups, but no remote ischemic conditioning was carried out. RIC 10: heart and brain were harvested 10 minutes after the remote ischemic conditioning protocol. RIC 60: heart and brain were harvested 60 minutes after the remote ischemic conditioning protocol. The remote ischemic conditioning protocol consisted of 3 cycles of 5 min left hindlimb ischemia followed by 5 min left hindlimb perfusion, lasting 30 min in total. Heart and brain samples were used to measure the tissue antioxidant capacity.  Results Remote ischemic conditioning increased heart and brain antioxidant capacity after 10 minutes (0.746 \u00b1 0.160\/0.801 \u00b1 0.227 mM\/L) when compared to SHAM (0.523 \u00b1 0.078\/0.404 \u00b1 0.124 mM\/L). No enhancement of heart or brain antioxidant capacity was detected 60 minutes after remote ischemic conditioning (0.551 \u00b1 0.073\/0.455 \u00b1 0.107 mM\/L).  Conclusions Remote ischemic conditioning temporarily enhances heart and brain antioxidant defenses in male Wistar rats.<\/jats:p>","DOI":"10.1590\/1677-5449.190129","type":"journal-article","created":{"date-parts":[[2020,5,11]],"date-time":"2020-05-11T19:59:09Z","timestamp":1589227149000},"source":"Crossref","is-referenced-by-count":0,"title":["Remote ischemic conditioning enhances heart and brain antioxidant defense"],"prefix":"10.1590","volume":"19","author":[{"given":"Felipe Lobato da Silva","family":"Costa","sequence":"first","affiliation":[{"name":"Universidade do Estado do Par\u00e1,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5079-297X","authenticated-orcid":false,"given":"Renan Kleber Costa","family":"Teixeira","sequence":"additional","affiliation":[{"name":"Universidade do Estado do Par\u00e1,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vitor Nagai","family":"Yamaki","sequence":"additional","affiliation":[{"name":"Universidade do Estado do Par\u00e1,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andr\u00e9 Lopes","family":"Valente","sequence":"additional","affiliation":[{"name":"Universidade do Estado do Par\u00e1,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandro","family":"Perc\u00e1rio","sequence":"additional","affiliation":[{"name":"Universidade Federal do Par\u00e1,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcus Vinicius Henriques","family":"Brito","sequence":"additional","affiliation":[{"name":"Universidade do Estado do Par\u00e1,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","published-online":{"date-parts":[[2020]]},"reference":[{"issue":"9","key":"ref1","doi-asserted-by":"crossref","first-page":"74","DOI":"10.4330\/wjc.v10.i9.74","article-title":"Myocardial reperfusion injury and oxidative stress: therapeutic opportunities","volume":"10","author":"Gonz\u00e1lez-Montero J","year":"2018","journal-title":"World J Cardiol"},{"issue":"10","key":"ref2","doi-asserted-by":"crossref","first-page":"2794","DOI":"10.1161\/STROKEAHA.112.660373","article-title":"Remote ischemic perconditioning is effective alone and in combination with intravenous tissue-type plasminogen activator in murine model of embolic stroke","volume":"43","author":"Hoda MN","year":"2012","journal-title":"Stroke"},{"issue":"6","key":"ref3","first-page":"586","article-title":"Preconditioning and postconditioning reduce hepatic ischemia-reperfusion injury in rats","volume":"8","author":"Zhang WX","year":"2009","journal-title":"Hepatobiliary Pancreat Dis Int"},{"issue":"1","key":"ref4","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1016\/j.cardiores.2004.01.006","article-title":"Postconditioning attenuates myocardial ischemia-reperfusion injury by inhibiting events in the early minutes of reperfusion","volume":"62","author":"Kin H","year":"2004","journal-title":"Cardiovasc Res"},{"issue":"4","key":"ref5","doi-asserted-by":"crossref","first-page":"364","DOI":"10.1111\/j.1432-2277.2007.00606.x","article-title":"Ischemic postconditioning inhibits apoptosis after renal ischemia\/reperfusion injury in rat","volume":"21","author":"Chen H","year":"2008","journal-title":"Transpl Int"},{"issue":"5","key":"ref6","first-page":"13","article-title":"Remote ischemic conditioning in ST-segment elevation myocardial infarction - an update","volume":"1","author":"Chong J","year":"2018","journal-title":"Cond Med."},{"issue":"6","key":"ref7","first-page":"529","article-title":"Protective effect of remote ischemic preconditioning in the ischemia and reperfusion-induced renal injury in rats","volume":"39","author":"Yamaki VN","year":"2012","journal-title":"Rev Col Bras Cir"},{"issue":"1","key":"ref8","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1016\/j.jss.2014.05.046","article-title":"Combined remote ischemic perconditioning and local postconditioning on liver ischemia-reperfusion injury","volume":"192","author":"Costa FL","year":"2014","journal-title":"J Surg Res"},{"issue":"3","key":"ref9","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1007\/s00395-019-0732-3","article-title":"Attenuation of ST-segment elevation after ischemic conditioning maneuvers reflects cardioprotection online","volume":"114","author":"Amanakis G","year":"2019","journal-title":"Basic Res Cardiol"},{"issue":"4","key":"ref10","doi-asserted-by":"crossref","first-page":"1883","DOI":"10.1152\/ajpheart.00617.2006","article-title":"Intermittent peripheral tissue ischemia during coronary ischemia reduces myocardial infarction through a KATP-dependent mechanism: first demonstration of remote ischemic perconditioning","volume":"292","author":"Schmidt MR","year":"2007","journal-title":"Am J Physiol Heart Circ Physiol"},{"issue":"10","key":"ref11","doi-asserted-by":"crossref","first-page":"1055","DOI":"10.1016\/j.jcin.2013.05.011","article-title":"Remote ischemic post-conditioning of the lower limb during primary percutaneous coronary intervention safely reduces enzymatic infarct size in anterior myocardial infarction: a randomized controlled trial","volume":"6","author":"Crimi G","year":"2013","journal-title":"JACC Cardiovasc Interv"},{"issue":"3-4","key":"ref12","doi-asserted-by":"crossref","first-page":"304","DOI":"10.1177\/1074248411411711","article-title":"Remote ischemic conditioning: a clinical trial\u2019s update","volume":"16","author":"Candilio L","year":"2011","journal-title":"J Cardiovasc Pharmacol Ther"},{"issue":"2","key":"ref13","doi-asserted-by":"crossref","first-page":"797","DOI":"10.1016\/j.jss.2012.06.067","article-title":"Remote ischemic preconditioning a simple, low-risk method to decrease ischemic reperfusion injury: models, protocols and mechanistic background: a review","volume":"178","author":"Szij\u00e1rt\u00f3 A","year":"2012","journal-title":"J Surg Res"},{"issue":"2","key":"ref14","doi-asserted-by":"crossref","first-page":"642","DOI":"10.1016\/j.jss.2014.08.046","article-title":"Neural elements behind the hepatoprotection of remote perconditioning","volume":"193","author":"Czig\u00e1ny Z","year":"2015","journal-title":"J Surg Res"},{"issue":"5","key":"ref15","doi-asserted-by":"crossref","first-page":"651","DOI":"10.1007\/s00395-010-0099-y","article-title":"The neural and humoral pathways in remote limb ischemic preconditioning","volume":"105","author":"Lim SY","year":"2010","journal-title":"Basic Res Cardiol"},{"issue":"6","key":"ref16","doi-asserted-by":"crossref","first-page":"1329","DOI":"10.1007\/s00395-011-0210-z","article-title":"RISK and SAFE signaling pathway interactions in remote limb ischemic perconditioning in combination with local ischemic postconditioning","volume":"106","author":"Tamareille S","year":"2011","journal-title":"Basic Res Cardiol"},{"issue":"2","key":"ref17","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1097\/SHK.0000000000000500","article-title":"Remote ischemic conditioning influences mitochondrial dynamics","volume":"45","author":"Cellier L","year":"2016","journal-title":"Shock"},{"issue":"1","key":"ref18","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/j.jss.2015.07.031","article-title":"Remote ischemic conditioning temporarily improves antioxidant defense","volume":"200","author":"Costa FL","year":"2016","journal-title":"J Surg Res"},{"issue":"4","key":"ref19","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1042\/cs0840407","article-title":"A novel method for measuring antioxidant capacity and its application to monitoring the antioxidant status in premature neonates","volume":"84","author":"Miller NJ","year":"1993","journal-title":"Clin Sci"},{"issue":"9-10","key":"ref20","doi-asserted-by":"crossref","first-page":"1231","DOI":"10.1016\/S0891-5849(98)00315-3","article-title":"Antioxidant activity applying an improved ABTS radical cation decolorization assay","volume":"26","author":"Re R","year":"1999","journal-title":"Free Radic Biol Med"},{"issue":"6","key":"ref21","doi-asserted-by":"crossref","first-page":"1841","DOI":"10.1021\/jf030723c","article-title":"The chemistry behind antioxidant capacity assays","volume":"53","author":"Huang D","year":"2005","journal-title":"J Agric Food Chem"},{"issue":"10","key":"ref22","doi-asserted-by":"crossref","first-page":"2960","DOI":"10.1161\/STROKEAHA.111.622340","article-title":"Remote ischemic per-conditioning: a novel therapy for acute stroke?","volume":"42","author":"Hahn CD","year":"2011","journal-title":"Stroke"},{"issue":"1","key":"ref23","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1016\/j.jcin.2014.05.015","article-title":"Remote ischemic conditioning reduces myocardial infarct size and edema in patients with ST-segment elevation myocardial infarction","volume":"8","author":"White SK","year":"2015","journal-title":"JACC Cardiovasc Interv"},{"issue":"5","key":"ref24","doi-asserted-by":"crossref","first-page":"1412","DOI":"10.1161\/STROKEAHA.116.016429","article-title":"RECAST (Remote Ischemic Conditioning After Stroke Trial): a pilot randomized placebo controlled phase II trial in acute ischemic stroke","volume":"48","author":"England TJ","year":"2017","journal-title":"Stroke"},{"issue":"4","key":"ref25","first-page":"370","article-title":"The effects of remote ischemic conditioning in patients with ST-segment elevation myocardial infarction treated with primary percutaneous coronary intervention: a meta-analysis","volume":"108","author":"Gao J","year":"2017","journal-title":"Minerva Med"}],"container-title":["Jornal Vascular Brasileiro"],"original-title":[],"link":[{"URL":"http:\/\/www.scielo.br\/pdf\/jvb\/v19\/1677-5449-jvb-19-e20190129.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,1,23]],"date-time":"2021-01-23T18:23:28Z","timestamp":1611426208000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_arttext&pid=S1677-54492020000100310&tlng=en"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020]]},"references-count":25,"alternative-id":["S1677-54492020000100310"],"URL":"https:\/\/doi.org\/10.1590\/1677-5449.190129","relation":{},"ISSN":["1677-7301","1677-5449"],"issn-type":[{"type":"electronic","value":"1677-7301"},{"type":"print","value":"1677-5449"}],"subject":[],"published":{"date-parts":[[2020]]},"article-number":"e20190129"}}