{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T15:08:33Z","timestamp":1753888113911,"version":"3.41.2"},"reference-count":16,"publisher":"FapUNIFESP (SciELO)","issue":"5","license":[{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rev. Assoc. Med. Bras."],"abstract":"<jats:p>SUMMARY  OBJECTIVE: The failure to conceive following 12 months of unprotected intercourse is referred to as infertility. About 35% of the instances include women and 30% involve men.  AIM: The aim of this study was to investigate the gene polymorphisms FASL-844C\/T and FAS-670A\/G in the promoter region to evaluate susceptibility to male infertility.  METHODS: We have investigated single-nucleotide polymorphisms of FAS-670A\/G gene and FASL-844C\/T gene in 100 subjects. A total of 50 male patients with infertility constituted the infertility group and another group consisting of 50 apparently healthy individuals comprised the control group.  RESULTS: The prevalence rate of FAS-670A\/G gene polymorphism was statistically significant among both azoospermia and oligospermia subgroups, the homozygous mutant genotype GG (p=0.014 and p=0.043), and mutant allele G (p=0.001 and p=0.004), respectively. Also, FASL-844C\/T gene polymorphism was statistically significant among both azoospermia and oligospermia subgroups, the homozygous mutant genotype TT (p=0.032 and p=0.032), and mutant allele T (p=0.007 and p=0.002), respectively.  CONCLUSIONS: FAS homozygous mutant genotype (GG) and FASL homozygous mutant genotype (TT) act as etiologic factors in male infertility.<\/jats:p>","DOI":"10.1590\/1806-9282.20241860","type":"journal-article","created":{"date-parts":[[2025,6,16]],"date-time":"2025-06-16T12:02:56Z","timestamp":1750075376000},"source":"Crossref","is-referenced-by-count":0,"title":["Genotyping of the gene polymorphisms FASL 844C\/T and FAS 670A\/G in patients with idiopathic infertility in Iraq"],"prefix":"10.1590","volume":"71","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0991-5077","authenticated-orcid":false,"given":"Abbas Ali","family":"Manshd","sequence":"first","affiliation":[{"name":"Sawa University,  Iraq"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-4719-0889","authenticated-orcid":false,"given":"Mohammed Ali","family":"Alaboudi","sequence":"additional","affiliation":[{"name":"Sawa University,  Iraq"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6893-860X","authenticated-orcid":false,"given":"Abbas Abdulameer","family":"Al-Raad","sequence":"additional","affiliation":[{"name":"Sawa University,  Iraq; Al-Muthanna University,  Iraq"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","published-online":{"date-parts":[[2025]]},"reference":[{"edition":"3rd ed","year":"2013","series-title":"Laboratory manual for the examination of human semen and semen cervical mucus interaction","key":"ref1"},{"issue":"3","key":"ref2","first-page":"CD007411","article-title":"Antioxidants for male subfertility","volume":"3","author":"Smits RM","year":"2019","journal-title":"Cochrane Database Syst Rev"},{"issue":"1","key":"ref3","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1002\/ijgo.12898","article-title":"Prognostic factors for pregnancy after intrauterine insemination","volume":"147","author":"Ejzenberg D","year":"2019","journal-title":"Int J Gynaecol Obstet"},{"issue":"1","key":"ref4","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/j.ejogrb.2012.10.021","article-title":"Evaluation of FAS and caspase-3 in the endometrial tissue of patients with idiopathic infertility and recurrent pregnancy loss","volume":"167","author":"Panzan MQ","year":"2013","journal-title":"Eur J Obstet Gynecol Reprod Biol"},{"issue":"6","key":"ref5","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1089\/gtmb.2013.0454","article-title":"Possible association of FAS and FASLG polymorphisms with the risk of idiopathic azoospermia in southeast Turkey","volume":"18","author":"Balkan M","year":"2014","journal-title":"Genet Test Mol Biomarkers"},{"issue":"1","key":"ref6","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1620\/tjem.234.17","article-title":"Polymorphisms of the apoptosis-related FAS and FAS ligand genes in keratoconus and Fuchs endothelial corneal dystrophy","volume":"234","author":"Synowiec E","year":"2014","journal-title":"Tohoku J Exp Med"},{"issue":"15","key":"ref7","first-page":"4327","article-title":"Functional FAS promoter polymorphisms are associated with increased risk of acute myeloid leukemia","volume":"63","author":"Sibley K","year":"2003","journal-title":"Cancer Res"},{"issue":"4","key":"ref8","first-page":"391","article-title":"Association analysis of FAS-670A\/G and FASL-844C\/T polymorphisms with risk of generalized aggressive periodontitis disease","volume":"8","author":"Asgari R","year":"2017","journal-title":"Biomed Rep"},{"issue":"1","key":"ref9","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/S1472-6483(10)60436-1","article-title":"FAS and FASLG polymorphisms and susceptibility to idiopathic azoospermia or severe oligozoospermia","volume":"18","author":"Wang W","year":"2009","journal-title":"Reprod Biomed Online"},{"issue":"8","key":"ref10","doi-asserted-by":"crossref","first-page":"5935","DOI":"10.1007\/s11033-021-06593-6","article-title":"Pro-inflammatory cytokines and microRNAs in male infertility","volume":"48","author":"Attia H","year":"2021","journal-title":"Mol Biol Rep"},{"issue":"9","key":"ref11","first-page":"981","article-title":"Blind aspiration biopsy versus a guided hysteroscopic technique for investigation of the endometrium in infertile women","volume":"31","author":"Ejzenberg D","year":"2016","journal-title":"Histol Histopathol"},{"issue":"1","key":"ref12","doi-asserted-by":"crossref","first-page":"024","DOI":"10.17352\/gjfr.000008","article-title":"The effects of semen parameters and age on sperm motility of Iranian men","volume":"2","author":"Shabani K","year":"2017","journal-title":"Glob J Fertil Res"},{"issue":"4","key":"ref13","doi-asserted-by":"crossref","first-page":"319","DOI":"10.4103\/UA.UA_50_20","article-title":"A population-based study of the epidemiology and the risk factors for male infertility in Kuwait","volume":"12","author":"Al-Kandari AM","year":"2020","journal-title":"Urol Ann"},{"issue":"2","key":"ref14","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/j.ucl.2019.12.001","article-title":"Cutting-edge evaluation of male infertility","volume":"47","author":"Pathak UI","year":"2020","journal-title":"Urol Clin North Am"},{"issue":"4","key":"ref15","first-page":"60","article-title":"FAS and FASL genes polymorphisms and their relationship with the incidence of severe oligozoospermia in a sample of Iraqi patients","volume":"16","author":"Hassan GM","year":"2017","journal-title":"Iraqi J Biotechnol"},{"issue":"10","key":"ref16","doi-asserted-by":"crossref","first-page":"2439","DOI":"10.1093\/humrep\/dep223","article-title":"Polymorphisms in cell death pathway genes are associated with altered sperm apoptosis and poor semen quality","volume":"24","author":"Ji G","year":"2009","journal-title":"Hum Reprod"}],"container-title":["Revista da Associa\u00e7\u00e3o M\u00e9dica Brasileira"],"original-title":[],"link":[{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_pdf&pid=S0104-42302025000500610&tlng=en","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,6,16]],"date-time":"2025-06-16T12:03:00Z","timestamp":1750075380000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_arttext&pid=S0104-42302025000500610&tlng=en"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025]]},"references-count":16,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2025]]}},"alternative-id":["S0104-42302025000500610"],"URL":"https:\/\/doi.org\/10.1590\/1806-9282.20241860","relation":{},"ISSN":["1806-9282","0104-4230"],"issn-type":[{"type":"electronic","value":"1806-9282"},{"type":"print","value":"0104-4230"}],"subject":[],"published":{"date-parts":[[2025]]},"article-number":"e20241860"}}