{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T18:42:21Z","timestamp":1775068941428,"version":"3.50.1"},"reference-count":18,"publisher":"FapUNIFESP (SciELO)","issue":"12","license":[{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Research Fund of the Ayd\u0131n Adnan Menderes University","award":["ADU BAP; TPF-24016"],"award-info":[{"award-number":["ADU BAP; TPF-24016"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rev. Assoc. Med. Bras."],"abstract":"<jats:p>SUMMARY  OBJECTIVE: Despite the longstanding clinical use of low-molecular-weight heparins and their well-characterized mechanisms of action, their effects on mitophagy pathways in acute myocardial ischemia remain incompletely delineated. The aim of this study was to investigate the enoxaparin effect on cardiomyocyte morphology and the immunopositivity of mitophagy-associated Parkin\u2013LC3 proteins in a rat model of acute myocardial ischemia.  METHODS: Female Wistar albino rats (median weight: 400 g, minimum: 375 g-maximum: 415 g) were divided into sham (n=7), control (n=7), and treatment (n=7) groups. The control group received subcutaneous 0.9% saline (0.2 mL\/kg) twice daily for 28 days, followed by the induction of myocardial ischemia. The treatment group was administered subcutaneous enoxaparin sodium (1 mg\/kg) twice daily for the same duration before undergoing myocardial ischemia. Myocardial ischemia was induced by occluding the proximal left anterior descending coronary artery using a 6-0 polypropylene suture for 10 min. Morphometric calculations of cardiomyocytes (cell length and diameter) were performed using light microscopy. Immunohistochemical analysis was performed using anti-Parkin and anti-LC3 antibodies, and immunopositive cells were counted.  RESULTS: Statistically significant differences were found between the groups (p&lt;0.001). Post-hoc analysis revealed significant differences between the control and treatment groups based on cell length, cell diameter, Parkin, and LC3 values (p&lt;0.001, p=0.001, p&lt;0.001, and p=0.001, respectively).  CONCLUSION: This study demonstrated that enoxaparin preserved cardiomyocyte morphology and reduced the number of Parkin\u2013LC3 immunopositive cells in a model of induced acute ischemic injury in rat myocardium.<\/jats:p>","DOI":"10.1590\/1806-9282.20251136","type":"journal-article","created":{"date-parts":[[2025,12,15]],"date-time":"2025-12-15T15:09:12Z","timestamp":1765811352000},"source":"Crossref","is-referenced-by-count":1,"title":["Enoxaparin preserves cellular morphology and modulates mitophagy-associated Parkin\u2013LC3 in acute ischemic rat myocardium"],"prefix":"10.1590","volume":"71","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2913-1170","authenticated-orcid":false,"given":"Fevzi","family":"Ayy\u0131ld\u0131z","sequence":"first","affiliation":[{"name":"Ayd\u0131n Adnan Menderes University,  T\u00fcrkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2303-6925","authenticated-orcid":false,"given":"U\u011fur","family":"Karag\u00f6z","sequence":"additional","affiliation":[{"name":"Ayd\u0131n Adnan Menderes University,  T\u00fcrkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2168-2891","authenticated-orcid":false,"given":"T\u00fcnay","family":"Kurto\u011flu","sequence":"additional","affiliation":[{"name":"Ayd\u0131n Adnan Menderes University,  T\u00fcrkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0915-8694","authenticated-orcid":false,"given":"P\u0131nar","family":"Akokay","sequence":"additional","affiliation":[{"name":"\u0130zmir Kavram Vocational School,  T\u00fcrkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9158-5627","authenticated-orcid":false,"given":"\u00c7a\u011fatay","family":"Bilen","sequence":"additional","affiliation":[{"name":"Ayd\u0131n Adnan Menderes University,  T\u00fcrkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","published-online":{"date-parts":[[2025]]},"reference":[{"issue":"5","key":"ref1","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1097\/CRD.0000000000000440","article-title":"Pathobiology of myocardial ischemia and reperfusion injury: models, modes, molecular mechanisms, modulation, and clinical applications","volume":"31","author":"Buja LM","year":"2023","journal-title":"Cardiol Rev"},{"issue":"2","key":"ref2","doi-asserted-by":"crossref","first-page":"736","DOI":"10.7150\/thno.79876","article-title":"Cellular mitophagy: mechanism, roles in diseases and small molecule pharmacological 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