{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T11:06:25Z","timestamp":1790593585372,"version":"4.1.0"},"reference-count":23,"publisher":"FapUNIFESP (SciELO)","issue":"9","license":[{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rev. Assoc. Med. Bras."],"abstract":"<jats:p>SUMMARY  OBJECTIVE: The aim of the study was to determine the association between plasma expression levels of microRNAs (miRNAs; MIR126-3p; and MIR375-3p) and pregnancy complications.  METHODS: This prospective study included 39 pregnant women who admitted to Obstetrics and Gynecology Polyclinic of Tokat Gaziosmanpasa University, Faculty of Medicine. 28 of these pregnant women were healthy, while 11 had pregnancy complications (five hypertensive disorders of pregnancy and six gestational diabetes). Hypertensive disorders include preeclampsia, gestational hypertension, and chronic hypertension. The study included pregnant women older than 18 years of age with singleton pregnancies and no history of cancer. Quantitative real time-polymerase chain reaction was used to determine the expression patterns of MIR126-3p and MIR375-3p in maternal plasma. Fold change (2-\u0394\u0394Ct) analysis was used to examine the microRNA expression patterns.  RESULTS: MIR375-3p expression levels demonstrated a statistically significant difference between two groups. It was significantly lower in women with pregnancy complications than healthy pregnant women.  CONCLUSION: We found that MIR375-3p might be a useful indicator for non-invasive diagnosis and play a crucial part in predicting pregnancy complications. Given the limited patient cohort, more research using bigger samples is required to validate our findings.<\/jats:p>","DOI":"10.1590\/1806-9282.20260435","type":"journal-article","created":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T10:50:54Z","timestamp":1790592654000},"source":"Crossref","is-referenced-by-count":0,"title":["Investigation of the relationship between expression patterns of plasma microRNAs and pregnancy complications"],"prefix":"10.1590","volume":"72","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1916-7471","authenticated-orcid":false,"given":"Nevin","family":"Karakus","sequence":"first","affiliation":[{"name":"Tokat Gaziosmanpasa University","place":["Tokat, Turkey"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7153-0226","authenticated-orcid":false,"given":"Elif Donmezoglu","family":"Kutukcu","sequence":"additional","affiliation":[{"name":"Tokat Gaziosmanpasa University","place":["Tokat, Turkey"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-5402-3140","authenticated-orcid":false,"given":"Sumeyye","family":"Yildirim","sequence":"additional","affiliation":[{"name":"Tokat Gaziosmanpasa University","place":["Tokat, Turkey"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"530","published-online":{"date-parts":[[2026]]},"reference":[{"issue":"5","key":"ref1","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1038\/ajh.2008.20","article-title":"Secular trends in the rates of preeclampsia, eclampsia, and gestational hypertension, United States, 1987-2004","volume":"21","author":"Wallis AB","year":"2008","journal-title":"Am J Hypertens"},{"issue":"8","key":"ref2","doi-asserted-by":"crossref","first-page":"999","DOI":"10.1586\/14737159.2015.1053468","article-title":"miRNAs in pregnancy-related complications","volume":"15","author":"Lycoudi A","year":"2015","journal-title":"Expert Rev Mol Diagn"},{"issue":"12","key":"ref3","doi-asserted-by":"crossref","first-page":"2890","DOI":"10.3390\/ijms20122890","article-title":"Exosomes-associated DNA-new marker in pregnancy complications?","volume":"20","author":"Kone\u010dn\u00e1 B","year":"2019","journal-title":"Int J Mol Sci"},{"issue":"24","key":"ref4","doi-asserted-by":"crossref","first-page":"13634","DOI":"10.3390\/ijms252413634","article-title":"Epigenetic biomarkers in thrombophilia-related pregnancy complications: mechanisms, diagnostic potential, and therapeutic implications: a narrative review","volume":"25","author":"Bardan CR","year":"2024","journal-title":"Int J Mol Sci"},{"issue":"1","key":"ref5","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1111\/imr.13074","article-title":"Extracellular vesicles and immune response during pregnancy: a balancing act","volume":"308","author":"Morelli AE","year":"2022","journal-title":"Immunol Rev"},{"issue":"6","key":"ref6","doi-asserted-by":"crossref","first-page":"1785","DOI":"10.1042\/BST20220734","article-title":"Placental extracellular vesicles in maternal-fetal communication during pregnancy","volume":"50","author":"Martin C","year":"2022","journal-title":"Biochem Soc Trans"},{"issue":"3","key":"ref7","doi-asserted-by":"crossref","first-page":"582","DOI":"10.3892\/etm.2022.11518","article-title":"Exosomes and exosomal non-coding RNAs throughout human gestation (Review)","volume":"24","author":"Maligianni I","year":"2022","journal-title":"Exp Ther Med"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"1080419","DOI":"10.3389\/fcell.2023.1080419","article-title":"Associations of maternal and placental extracellular vesicle miRNA with preeclampsia","volume":"11","author":"Aharon A","year":"2023","journal-title":"Front Cell Dev Biol"},{"issue":"1","key":"ref9","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1038\/s41392-024-01735-1","article-title":"Extracellular vesicles as tools and targets in therapy for diseases","volume":"9","author":"Kumar MA","year":"2024","journal-title":"Signal Transduct Target Ther"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"104255","DOI":"10.1016\/j.jri.2024.104255","article-title":"Antiphospholipid-exposed trophoblast-derived extracellular vesicles express elevated levels of TLR7\/8-activating microRNAs and induce endometrial endothelial activation, in part, through TLR7","volume":"164","author":"Coenen CS","year":"2024","journal-title":"J Reprod Immunol"},{"issue":"4","key":"ref11","doi-asserted-by":"crossref","first-page":"864","DOI":"10.1161\/HYPERTENSIONAHA.111.203489","article-title":"MicroRNA-376c impairs transforming growth factor-\u03b2 and nodal signaling to promote trophoblast cell proliferation and invasion","volume":"61","author":"Fu G","year":"2013","journal-title":"Hypertension"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/j.trsl.2016.02.012","article-title":"MicroRNA-141 is upregulated in preeclamptic placentae and regulates trophoblast invasion and intercellular communication","volume":"172","author":"Ospina-Prieto S","year":"2016","journal-title":"Transl Res"},{"issue":"1","key":"ref13","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/j.ncrna.2021.03.001","article-title":"Analysis of differential expression of hypoxia-inducible microRNA-210 gene targets in mild and severe preeclamptic patients","volume":"6","author":"Jairajpuri DS","year":"2021","journal-title":"Noncoding RNA Res"},{"issue":"1","key":"ref14","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1186\/s12884-024-06371-9","article-title":"MicroRNA-223-3p downregulates the inflammatory response in preeclampsia placenta via targeting NLRP3","volume":"24","author":"Liu X","year":"2024","journal-title":"BMC Pregnancy Childbirth"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"103281","DOI":"10.1016\/j.jri.2021.103281","article-title":"Downregulation of miR-126-3p expression contributes to increased inflammatory response in placental trophoblasts in preeclampsia","volume":"144","author":"Chu X","year":"2021","journal-title":"J Reprod Immunol"},{"issue":"1","key":"ref16","doi-asserted-by":"crossref","first-page":"1350","DOI":"10.1080\/07853890.2022.2071459","article-title":"The role of miRNA-210 in pre-eclampsia development","volume":"54","author":"Jaszczuk I","year":"2022","journal-title":"Ann Med"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1016\/j.ejogrb.2023.09.012","article-title":"The role and expression of pro\/antiangiogenic factors and microRNAs in gestational hypertension and pre-eclampsia","volume":"290","author":"Soobryan N","year":"2023","journal-title":"Eur J Obstet Gynecol Reprod Biol"},{"issue":"3","key":"ref18","doi-asserted-by":"crossref","first-page":"803","DOI":"10.1007\/s43032-023-01367-w","article-title":"Are vascular endothelium and angiogenesis effective MicroRNA biomarkers associated with the prediction of early-onset preeclampsia (EOPE) and adverse perinatal outcomes?","volume":"31","author":"Ozler S","year":"2024","journal-title":"Reprod Sci"},{"issue":"2","key":"ref19","doi-asserted-by":"crossref","first-page":"150","DOI":"10.3109\/10641955.2015.1122034","article-title":"Plasma levels of increased miR-195-5p correlates with the sFLT-1 levels in preeclampsia","volume":"35","author":"Sandrim VC","year":"2016","journal-title":"Hypertens Pregnancy"},{"issue":"6","key":"ref20","doi-asserted-by":"crossref","first-page":"910","DOI":"10.1080\/01443615.2020.1820465","article-title":"Expression levels of maternal plasma microRNAs in preeclamptic pregnancies","volume":"41","author":"Akg\u00f6r U","year":"2021","journal-title":"J Obstet Gynaecol"},{"issue":"1","key":"ref21","doi-asserted-by":"crossref","first-page":"16770","DOI":"10.1038\/s41598-024-64057-w","article-title":"Circulating extracellular vesicular microRNA signatures in early gestation show an association with subsequent clinical features of pre-eclampsia","volume":"14","author":"Ghosh S","year":"2024","journal-title":"Sci Rep"},{"issue":"3","key":"ref22","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1016\/j.gde.2009.04.002","article-title":"AngiomiRs--key regulators of angiogenesis","volume":"19","author":"Wang S","year":"2009","journal-title":"Curr Opin Genet Dev"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"119323","DOI":"10.1016\/j.lfs.2021.119323","article-title":"MiR-375: a novel multifunctional regulator","volume":"275","author":"Liu Y","year":"2021","journal-title":"Life Sci"}],"container-title":["Revista da Associa\u00e7\u00e3o M\u00e9dica Brasileira"],"original-title":[],"link":[{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_pdf&pid=S0104-42302026000902205&tlng=en","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T10:50:57Z","timestamp":1790592657000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_arttext&pid=S0104-42302026000902205&tlng=en"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026]]},"references-count":23,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2026]]}},"alternative-id":["S0104-42302026000902205"],"URL":"https:\/\/doi.org\/10.1590\/1806-9282.20260435","relation":{},"ISSN":["1806-9282","0104-4230"],"issn-type":[{"value":"1806-9282","type":"electronic"},{"value":"0104-4230","type":"print"}],"subject":[],"published":{"date-parts":[[2026]]},"article-number":"e20260435"}}