{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,8,15]],"date-time":"2023-08-15T02:10:04Z","timestamp":1692065404831},"reference-count":36,"publisher":"FapUNIFESP (SciELO)","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rev. Assoc. Med. Bras."],"published-print":{"date-parts":[[2015,8]]},"abstract":"<jats:p>SummaryIntroduction:aging is associated with several immunologic changes. Regulatory (Treg) and effector T cells are involved in the pathogenesis of infectious, neoplastic, and autoimmune diseases. Little is known about the effects of aging on the frequency and function of these T cell subpopulations.Methods:peripheral blood mononuclear cells (PBMC) were obtained from 26 young (under 44 years old) and 18 elderly (above 80 years old) healthy women. T cell subpopulations were analyzed by flow cytometry.Results:elderly individuals had lower frequency of several activated effector T cell phenotypes as compared with young individuals: CD3+CD4+CD25+ (3.82\u00b11.93 versus 9.53\u00b14.49; p&lt;0.0001); CD3+CD4+CD25+CD127+(2.39\u00b11.19 versus 7.26\u00b13.84; p&lt;0.0001); CD3+CD4+CD25+ (0.41\u00b10.22 versus 1.86\u00b10.85, p&lt;0.0001); and CD3+CD4+CD25highCD127+(0.06\u00b10.038 versus 0.94\u00b10.64, p&lt;0.0001). Treg (CD3+CD4+CD25+CD127\u00f8Foxp3+) presented lower frequency in elderly individuals as compared to young adults (0.34\u00b10.18 versus 0.76\u00b10.48; p=0.0004) and its frequency was inversely correlated with age in the whole group (r=-0.439; p=0.013). The elderly group showed higher frequency of two undefined CD25\u00f8Foxp3+ phenotypes: CD3+CD4+CD25\u00f8Foxp3+(15.05\u00b17.34 versus 1.65\u00b11.71; p&lt;0.0001) and CD3+CD4+CD25\u00f8CD127\u00f8Foxp3+(13.0\u00b15.52 versus 3.51\u00b12.87; p&lt;0.0001).Conclusions:the altered proportion of different T cell subsets herein documented in healthy elderly women may be relevant to the understanding of the immunologic behavior and disease susceptibility patterns observed in geriatric patients.<\/jats:p>","DOI":"10.1590\/1806-9282.61.04.329","type":"journal-article","created":{"date-parts":[[2015,10,8]],"date-time":"2015-10-08T01:20:19Z","timestamp":1444267219000},"page":"329-335","source":"Crossref","is-referenced-by-count":0,"title":["Abnormal phenotypic distribution of regulatory and effector T cells in octogenarian and nonagenarian women"],"prefix":"10.1590","volume":"61","author":[{"given":"Wilson de Melo","family":"Cruvinel","sequence":"first","affiliation":[{"name":"Pontif\u00edcia Universidade Cat\u00f3lica de Goi\u00e1s, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Danilo","family":"Mesquita J\u00fanior","sequence":"additional","affiliation":[{"name":"Universidade Federal de S\u00e3o Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u00falio Ant\u00f4nio Pereira","family":"Ara\u00fajo","sequence":"additional","affiliation":[{"name":"Universidade Federal de S\u00e3o Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karina Carvalho","family":"Samazi","sequence":"additional","affiliation":[{"name":"Universidade de S\u00e3o Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Esper Georges","family":"K\u00e1llas","sequence":"additional","affiliation":[{"name":"Universidade de S\u00e3o Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maysa Seabra","family":"Cendoroglo","sequence":"additional","affiliation":[{"name":"Universidade Federal de S\u00e3o Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luis Eduardo Coelho","family":"Andrade","sequence":"additional","affiliation":[{"name":"Universidade Federal de S\u00e3o Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","reference":[{"issue":"9029","key":"ref1","doi-asserted-by":"crossref","first-page":"762","DOI":"10.1016\/S0140-6736(05)65680-2","article-title":"Vaccination against tetanus in the elderly: do recommended vaccination strategies give sufficient protection","volume":"348","author":"Steger MM","year":"1996","journal-title":"Lancet"},{"issue":"4","key":"ref2","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1023\/A:1006659401385","article-title":"The effect of age on the B-cell repertoire","volume":"20","author":"Weksler ME","year":"2000","journal-title":"J Clin Immunol"},{"issue":"11","key":"ref3","doi-asserted-by":"crossref","first-page":"7446","DOI":"10.4049\/jimmunol.174.11.7446","article-title":"The influence of age on T cell generation and TCR diversity","volume":"174","author":"Naylor K","year":"2005","journal-title":"J Immunol"},{"issue":"8","key":"ref4","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1016\/j.mad.2009.06.003","article-title":"Aging and human CD4(+) regulatory T cells","volume":"130","author":"Hwang KA","year":"2009","journal-title":"Mech Ageing Dev"},{"issue":"3","key":"ref5","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1159\/000052839","article-title":"On the role of aging in the etiology of autoimmunity","volume":"48","author":"Urb\u00e1n L","year":"2002","journal-title":"Gerontology"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"89195","DOI":"10.1155\/2007\/89195","article-title":"Regulatory T cells and human disease","volume":"2007","author":"Cools N","year":"2007","journal-title":"Clin Dev Immunol"},{"issue":"2","key":"ref7","doi-asserted-by":"crossref","first-page":"73","DOI":"10.14712\/18059694.2016.63","article-title":"Regulatory T cells (TREG) and their roles in immune system with respect to immunopathological disorders","volume":"53","author":"Kondelkov\u00e1 K","year":"2010","journal-title":"Acta Medica (Hradec Kralove)"},{"issue":"4","key":"ref8","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1097\/MOT.0b013e32833bfadc","article-title":"Adoptive regulatory T cell therapy: challenges in clinical transplantation","volume":"15","author":"Safinia N","year":"2010","journal-title":"Curr Opin Organ Transplant"},{"issue":"3","key":"ref9","doi-asserted-by":"crossref","first-page":"1151","DOI":"10.4049\/jimmunol.155.3.1151","article-title":"Immunologic self-tolerance maintained by activated T cells expressing IL-2 receptor alpha-chains (CD25). Breakdown of a single mechanism of self-tolerance causes various autoimmune diseases","volume":"155","author":"Sakaguchi S","year":"1995","journal-title":"J Immunol"},{"issue":"5609","key":"ref10","doi-asserted-by":"crossref","first-page":"1057","DOI":"10.1126\/science.1079490","article-title":"Control of regulatory T cell development by the transcription factor Foxp3","volume":"299","author":"Hori S","year":"2003","journal-title":"Science"},{"issue":"4","key":"ref11","doi-asserted-by":"crossref","first-page":"330","DOI":"10.1038\/ni904","article-title":"Foxp3 programs the development and function of CD4 CD25 regulatory T cells","volume":"4","author":"Fontenot JD","year":"2003","journal-title":"Nat Immunol"},{"issue":"4","key":"ref12","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1038\/ni909","article-title":"An essential role for Scurfin in CD4 CD25 T regulatory cells","volume":"4","author":"Khattri R","year":"2003","journal-title":"Nat Immunol"},{"issue":"12","key":"ref13","doi-asserted-by":"crossref","first-page":"8348","DOI":"10.4049\/jimmunol.177.12.8348","article-title":"High accumulation of T regulatory cells prevents the activation of immune responses in aged animals","volume":"177","author":"Sharma S","year":"2006","journal-title":"J Immunol"},{"issue":"6","key":"ref14","doi-asserted-by":"crossref","first-page":"1386","DOI":"10.1189\/jlb.0506364","article-title":"Changes of CD4 CD25 Foxp3 regulatory T cells in aged Balb\/c mice","volume":"81","author":"Zhao L","year":"2007","journal-title":"J Leukoc Biol"},{"issue":"11","key":"ref15","doi-asserted-by":"crossref","first-page":"6586","DOI":"10.4049\/jimmunol.176.11.6586","article-title":"CD4 CD25 Foxp3 T cells and CD4 CD25-Foxp3 T cells in aged mice","volume":"176","author":"Nishioka T","year":"2006","journal-title":"J Immunol"},{"issue":"4","key":"ref16","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1016\/j.mad.2011.03.001","article-title":"Expansion of regulatory T cells in aged mice following influenza infection","volume":"132","author":"Williams-Bey Y","year":"2011","journal-title":"Mech Ageing Dev"},{"issue":"3","key":"ref17","doi-asserted-by":"crossref","first-page":"540","DOI":"10.1111\/j.1365-2249.2005.02798.x","article-title":"The number of human peripheral blood CD4 CD25high regulatory T cells increases with age","volume":"140","author":"Gregg R","year":"2005","journal-title":"Clin Exp Immunol"},{"issue":"3","key":"ref18","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1016\/j.jaut.2005.01.015","article-title":"CD4 CD25high regulatory T cells are not impaired in patients with primary Sj\u00f6gren's syndrome","volume":"24","author":"Gottenberg JE","year":"2005","journal-title":"J Autoimmun"},{"issue":"3","key":"ref19","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1016\/j.clim.2006.02.002","article-title":"CD4 CD25 T regulatory cells inhibit cytotoxic activity of CTL and NK cells in humans-impact of immunosenescence","volume":"119","author":"Trzonkowski P","year":"2006","journal-title":"Clin Immunol"},{"issue":"9","key":"ref20","doi-asserted-by":"crossref","first-page":"2423","DOI":"10.1172\/JCI28941","article-title":"Human CD4 CD25hi Foxp3 regulatory T cells are derived by rapid turnover of memory populations in vivo","volume":"116","author":"Vukmanovic-stejic M","year":"2006","journal-title":"J Clin Invest"},{"issue":"3","key":"ref21","doi-asserted-by":"crossref","first-page":"1835","DOI":"10.4049\/jimmunol.181.3.1835","article-title":"Functional regulatory T cells accumulate in aged hosts and promote chronic infectious disease reactivation","volume":"181","author":"Lages CS","year":"2008","journal-title":"J Immunol"},{"issue":"4","key":"ref22","first-page":"S6","article-title":"Clinical features of GCA\/PMR","volume":"18","author":"Hunder GG","year":"2000","journal-title":"Clin Exp Rheumatol"},{"issue":"2","key":"ref23","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1016\/j.cellimm.2005.04.012","article-title":"Immune receptor signaling, aging, and autoimmunity","volume":"233","author":"Hasler P","year":"2005","journal-title":"Cell Immunol"},{"issue":"1","key":"ref24","doi-asserted-by":"crossref","first-page":"156","DOI":"10.4049\/jimmunol.1001505","article-title":"A major role for Bim in regulatory T cell homeostasis","volume":"186","author":"Chougnet CA","year":"2011","journal-title":"J Immunol"},{"issue":"1-2","key":"ref25","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1016\/j.jneuroim.2007.05.011","article-title":"Higher frequency of regulatory T cells in the elderly and increased suppressive activity in neurodegeneration","volume":"188","author":"Rosenkranz D","year":"2007","journal-title":"J Neuroimmunol"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1186\/1742-4933-7-4","article-title":"The effect of ageing on human lymphocyte subsets: comparison of males and females","volume":"7","author":"Yan J","year":"2010","journal-title":"Immun Ageing"},{"issue":"3","key":"ref27","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1093\/intimm\/dxm151","article-title":"Accelerated age-dependent transition of human regulatory T cells to effector memory phenotype","volume":"20","author":"Santner-Nanan B","year":"2008","journal-title":"Int Immunol"},{"issue":"4","key":"ref28","doi-asserted-by":"crossref","first-page":"482","DOI":"10.1016\/j.coi.2012.04.005","article-title":"Homeostasis and function of regulatory T cells in aging","volume":"24","author":"Raynor J","year":"2012","journal-title":"Curr Opin Immunol"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"231","DOI":"10.3389\/fimmu.2013.00231","article-title":"The impact of aging on regulatory T-cells","volume":"4","author":"Fessler J","year":"2013","journal-title":"Front Immunol"},{"issue":"1-2","key":"ref30","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.jim.2012.04.003","article-title":"A flow cytometric protocol for enumeration of endothelial progenitor cells and monocyte subsets in human blood","volume":"381","author":"Hristov M","year":"2012","journal-title":"J Immunol Methods"},{"issue":"6","key":"ref31","doi-asserted-by":"crossref","first-page":"1454","DOI":"10.1189\/jlb.0907627","article-title":"The frequency of regulatory CD3 CD8 CD28- CD25 T lymphocytes in human peripheral blood increases with age","volume":"84","author":"Simone R","year":"2008","journal-title":"J Leukoc Biol"},{"issue":"4","key":"ref32","doi-asserted-by":"crossref","first-page":"2572","DOI":"10.4049\/jimmunol.178.4.2572","article-title":"Expansion of CD4 CD25 and FOXP3 regulatory t cells during the follicular phase of the menstrual cycle: implications for human reproduction","volume":"178","author":"Arruvito L","year":"2006","journal-title":"J Immunol"},{"issue":"2","key":"ref33","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1016\/j.autrev.2005.09.008","article-title":"Aging of the immune system: a risk factor for autoimmunity?","volume":"5","author":"Prelog M","year":"2006","journal-title":"Autoimmun Rev"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"22","DOI":"10.2174\/1874312900903010022","article-title":"The nature of increased circulating CD4 CD25 Foxp3 T cells in patients with systemic lupus erythematosus: a novel hypothesis","volume":"3","author":"Yan B","year":"2009","journal-title":"Open Rheumatol J"},{"issue":"1","key":"ref35","doi-asserted-by":"crossref","first-page":"41","DOI":"10.3109\/03009742.2010.489229","article-title":"Systemic lupus erythematosus exhibits a dynamic and continuum spectrum of effector\/regulatory T cells","volume":"40","author":"Mesquita D","year":"2011","journal-title":"Scand J Rheumatol"},{"issue":"3","key":"ref36","doi-asserted-by":"crossref","first-page":"1689","DOI":"10.4049\/jimmunol.182.3.1689","article-title":"Phenotypic and functional analysis of CD4 CD25- Foxp3 T cells in patients with systemic lupus erythematosus","volume":"182","author":"Bonelli M","year":"2009","journal-title":"J Immunol"}],"container-title":["Revista da Associa\u00e7\u00e3o M\u00e9dica Brasileira"],"original-title":[],"link":[{"URL":"http:\/\/www.scielo.br\/pdf\/ramb\/v61n4\/0104-4230-ramb-61-04-0329.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,15]],"date-time":"2023-08-15T01:32:41Z","timestamp":1692063161000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_arttext&pid=S0104-42302015000400329&lng=en&tlng=en"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,8]]},"references-count":36,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2015,8]]}},"alternative-id":["S0104-42302015000400329"],"URL":"https:\/\/doi.org\/10.1590\/1806-9282.61.04.329","relation":{},"ISSN":["0104-4230"],"issn-type":[{"value":"0104-4230","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,8]]}}}