{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,28]],"date-time":"2025-09-28T11:12:23Z","timestamp":1759057943931,"version":"3.37.3"},"reference-count":25,"publisher":"FapUNIFESP (SciELO)","issue":"7","license":[{"start":{"date-parts":[[2019,7,1]],"date-time":"2019-07-01T00:00:00Z","timestamp":1561939200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2019,7,1]],"date-time":"2019-07-01T00:00:00Z","timestamp":1561939200000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2019,7,1]],"date-time":"2019-07-01T00:00:00Z","timestamp":1561939200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rev. Assoc. Med. Bras."],"abstract":"<jats:p>SUMMARY  OBJECTIVE  To investigate the relations of T lymphocytes, cytokines, immunoglobulin E, and nitric oxide with otitis media with effusion (OME) in children and their clinical significances.  METHODS  Fifty children with OME treated in our hospital were enrolled in the study (observation group). Fifty healthy children were selected as control. The percentages of CD4+ and CD8+ T lymphocyte and CD4+\/CD8+ ratio in peripheral blood, and the levels of cytokine (IL)-2, IL-4, IL-6, immunoglobulin E (IgE) and nitric oxide (NO) in peripheral blood and middle ear effusion (MEE) in both groups were detected. The correlations of these indexes with OME were analyzed.  RESULTS  The percentage of peripheral blood CD4+ and CD8+ levels, CD4+\/CD8 ratio, IgE, and NO levels in the observation group were significantly higher than those in the control group (P &lt; 0.01). In the observation group, the IL-2 and IL-6 levels, and IgE and NO levels in the MEE were significantly higher than those in peripheral blood (P &lt; 0.01). In addition, in the observation group, the MEE IL-2 and IL-6 levels were positively correlated with peripheral blood CD4+\/CD8+ ratio, respectively r = 0.366, P = 0.009; r = 0.334, P = 0.018.  CONCLUSIONS  The levels of peripheral blood CD4+ and CD8+ lymphocytes and MEE IL-2, IL-6, IgE, and NO levels are increased in children with OME. These indexes have provided significant clues for the diagnosis of OME in children.<\/jats:p>","DOI":"10.1590\/1806-9282.65.7.971","type":"journal-article","created":{"date-parts":[[2019,8,3]],"date-time":"2019-08-03T15:34:59Z","timestamp":1564846499000},"page":"971-976","source":"Crossref","is-referenced-by-count":7,"title":["Relationship of T lymphocytes, cytokines, immunoglobulin E and nitric oxide with otitis media with effusion in children and their clinical significances"],"prefix":"10.1590","volume":"65","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7918-3756","authenticated-orcid":false,"given":"Wenyan","family":"Fan","sequence":"first","affiliation":[{"name":"Shanghai Jiao Tong University,  China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3292-019X","authenticated-orcid":false,"given":"Xiaoyan","family":"Li","sequence":"additional","affiliation":[{"name":"Shanghai Jiao Tong University,  China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4683-1059","authenticated-orcid":false,"given":"Hongming","family":"Xu","sequence":"additional","affiliation":[{"name":"Shanghai Jiao Tong University,  China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5623-8817","authenticated-orcid":false,"given":"Limin","family":"Zhao","sequence":"additional","affiliation":[{"name":"Shanghai Jiao Tong University,  China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2736-1108","authenticated-orcid":false,"given":"Jiali","family":"Zhao","sequence":"additional","affiliation":[{"name":"Shanghai Jiao Tong University,  China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9746-1887","authenticated-orcid":false,"given":"Wanpeng","family":"Li","sequence":"additional","affiliation":[{"name":"Shanghai Jiao Tong 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