{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,20]],"date-time":"2025-05-20T04:06:35Z","timestamp":1747713995921,"version":"3.40.5"},"reference-count":38,"publisher":"FapUNIFESP (SciELO)","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["An. Bras. Dermatol."],"published-print":{"date-parts":[[2013,12]]},"abstract":"<jats:p>BACKGROUND: In highly endemic countries, transmission and sub-clinical infection of leprosy are likely and the disease manifests itself in individuals without any known close contact with a leprosy patient. Health workers are social contacts belonging to the same network (the Health System) and some of them share the same social environment (nursing assistants) as patients with known patients and \/ or carriers. OBJECTIVE: To identify ML Flow seropositivity among health professionals. METHODS: We conducted a cross-sectional study using a serological survey with the ML Flow test in 450 health professionals (doctors, nurses and nursing assistants), in order to detect seropositivity in areas of high and low endemicity in municipalities from three Brazilian states (RJ, MS and RS). RESULTS: The results showed general 16% seropositivity, higher in low endemic areas, regardless of whether there was direct care for leprosy patients. Paradoxically, a statistical association was observed between the area studied and seropositivity, as the place with the lowest endemicity (CA) had the highest seropositivity rate (p = 0.033). CONCLUSION: The authors suggest these results are associated with a presence of an unspecified link to bovine serum albumin (BSA), carrier of PGL-1 in the ML Flow test, and recommend expanded seroepidemiological research utilizing tests with human and bovine albumin.<\/jats:p>","DOI":"10.1590\/abd1806-4841.20132139","type":"journal-article","created":{"date-parts":[[2014,2,14]],"date-time":"2014-02-14T14:05:27Z","timestamp":1392386727000},"page":"918-923","source":"Crossref","is-referenced-by-count":2,"title":["Serology with ML Flow test in health professionals from three different states of Brazil"],"prefix":"10.1590","volume":"88","author":[{"given":"Karla Lucena Sampaio","family":"Calado","sequence":"first","affiliation":[{"name":"Federal University of Rio de Janeiro, Brazil; Federal University of Grande Dourados; Oswaldo Cruz Foundation"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M\u00f4nica Maria Ferreira","family":"Magnanini","sequence":"additional","affiliation":[{"name":"Oswaldo Cruz Foundation, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rodrigo Scaliante de","family":"Moura","sequence":"additional","affiliation":[{"name":"Federal University of Goi\u00e1s, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Eugenia Noviski","family":"Gallo","sequence":"additional","affiliation":[{"name":"Oswaldo Cruz Foundation, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samira","family":"B\u00fchrer-S\u00e9kula","sequence":"additional","affiliation":[{"name":"Universiteit van Amsterdam, Netherlands, Netherlands; Federal University of Goi\u00e1s, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Leide Wand-Del-Rey de","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Federal University of Rio de Janeiro, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1016\/S0873-2159(15)30004-0","article-title":"Tuberculosis - Risk of continued transmission in healthcare workers","volume":"16","author":"Costa J","year":"2010","journal-title":"Rev Port Pneumol"},{"key":"ref2","first-page":"347","article-title":"Epidemiological monitoring of tuberculosis in a general teaching hospital","volume":"36","author":"Gon\u00e7alves B","year":"2010","journal-title":"J Bras Pneumol"},{"year":"2000","series-title":"A Simple Dipstick Assay for Leprosy: Development, Evaluation and Application [tese]","author":"B\u00fchrer-S\u00e9kula S","key":"ref3"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"70","DOI":"10.47276\/lr.78.1.70","article-title":"The ML Flow test as a point of care test for leprosy control programmes: potential effects on classification of leprosy patients","volume":"78","author":"B\u00fchrer-S\u00e9kula S","year":"2007","journal-title":"Lepr Rev"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"1991","DOI":"10.1128\/JCM.41.5.1991-1995.2003","article-title":"Simple and fast lateral flow test for classification of leprosy patients and identification of contacts with high risk of developing leprosy","volume":"41","author":"B\u00fchrer-S\u00e9kula S","year":"2003","journal-title":"J Clin Microbiol"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"897","DOI":"10.1128\/CDLI.11.5.897-900.2004","article-title":"Prospective study of serological conversion as risk factor for development ofleprosy among household contacts","volume":"11","author":"Douglas JT","year":"2004","journal-title":"Clin Diagn Lab Immunol"},{"key":"ref7","first-page":"230","article-title":"Leprosy: too complex a disease for a simple elimination paradigm","volume":"83","author":"Lockwood DNJ","year":"2005","journal-title":"Bull World Health Organ"},{"key":"ref8","first-page":"243","article-title":"Seroepidemiological studies of leprosy in northen Malawi based on an enzyme-linked immunosorbent assay using synthetic glycoconjugate antigen","volume":"56","author":"Fine PE","year":"1988","journal-title":"Int J Lepr Other Mycobact Dis"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1016\/S0928-8244(98)00049-2","article-title":"The use of whole blood in a dipstick assay for detection of antibodies to Mycobacterium leprae: a Field evaluation","volume":"21","author":"B\u00fchrer-Sekula S","year":"1998","journal-title":"FEMS Immunol Med Microbiol"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"133","DOI":"10.4269\/ajtmh.1998.58.133","article-title":"A simple dipstick assay for the detection of antibodies to phenolic glycolipid-I of Mycobacterium leprae","volume":"58","author":"B\u00fchrer SS","year":"1998","journal-title":"Am J Trop Med Hyg"},{"key":"ref11","first-page":"279","article-title":"Rapid method for diagnosis of leprosy by measurements of antibodies to the M. leprae 35-kDa protein: comparison with PGL-I antibodies detected by ELISA and \"dipstick\" methods","volume":"67","author":"Roche PW","year":"1999","journal-title":"Int J Lepr Other Mycobact Dis"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1590\/S0037-86822008000700002","article-title":"PGL-I leprosy serology","volume":"41","author":"B\u00fchrer-S\u00e9kula S","year":"2008","journal-title":"Rev Soc Bras Med Trop"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"262","DOI":"10.1590\/S1415-790X2003000300010","article-title":"Sorologia Anti PGL-1 e risco de ocorr\u00eancia de hansen\u00edase em \u00e1rea de alta endemicidade do Estado de S\u00e3o Paulo: quatro anos de seguimento","volume":"6","author":"Brasil MTLRF","year":"2003","journal-title":"Rev Bras Epidemiol"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"1400","DOI":"10.1128\/CVI.00299-07","article-title":"Use of Protein Antigens for Early Serological Diagnosis of Leprosy","volume":"14","author":"Duthie MS","year":"2007","journal-title":"Clin Vaccine Immunol"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1214\/aoms\/1177705148","article-title":"Snowball Sampling","volume":"32","author":"Goodman LA","year":"1961","journal-title":"An. 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