{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T18:25:10Z","timestamp":1774981510954,"version":"3.50.1"},"reference-count":21,"publisher":"FapUNIFESP (SciELO)","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["An. Bras. Dermatol."],"published-print":{"date-parts":[[2013,10]]},"abstract":"<jats:p> BACKGROUND:  Patch testing remains the gold standard method for the identification of the etiologic agent of allergic contact dermatitis. However, it is a subjective, time-consuming exam whose technique demands special care and which presents some contraindications, which hamper its use. In a recent study, we showed that the proliferation assay can suitably replace patch testing for the diagnosis of chromium allergy, which had been previously demonstrated only for nickel allergy. In this study, we try to refine the method by reducing the incubation period of cultures for lymphocyte proliferation assays in response to chromium.    OBJECTIVE:  Develop an alternative or complementary diagnostic test for chromium allergic contact dermatitis.    METHODS:  We compared the production of 9 cytokines (IFN-\u03b3, IL-2, IL-4, IL-5, IL-10, IL-12, IL-13, IL-17 and RANTES) between 18 chromium-allergic patients and 19 controls.    RESULTS:  Chromium increased the production of IFN-y, IL-5, IL-2 and IL-13 in allergic patients, but only IL-2 and especially IL-13 helped discriminate allergic patients from controls. The sensitivity, specificity and accuracy found with IL-13 were about 80%.    CONCLUSIONS:  IL-13 and IL-2 detection may be used to diagnose chromium allergy in 2-day cultures. However, in general, the 6-day cultures seem to be superior for this purpose.<\/jats:p>","DOI":"10.1590\/abd1806-4841.20132164","type":"journal-article","created":{"date-parts":[[2013,11,25]],"date-time":"2013-11-25T13:35:47Z","timestamp":1385386547000},"page":"719-725","source":"Crossref","is-referenced-by-count":7,"title":["Cytokine detection for the diagnosis of chromium allergy"],"prefix":"10.1590","volume":"88","author":[{"given":"Luis Eduardo Agner Machado","family":"Martins","sequence":"first","affiliation":[{"name":"Faculdade Evangelica do Parana,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vitor Manoel Silva dos","family":"Reis","sequence":"additional","affiliation":[{"name":"University of Sao Paulo,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"763","DOI":"10.1007\/s00018-011-0846-8","article-title":"Allergic contact dermatitis: epidemiology, molecular mechanisms, in vitro methods and regulatory aspects. 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A multicenter study of synchronous left-versus right-sided patch tests by the German Contact Dermatitis Research Group","volume":"31","author":"Brasch J","year":"1994","journal-title":"J Am Acad Dermatol"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1111\/j.1600-0536.1985.tb01060.x","article-title":"False-positive, follicular and irritant patch test reactions to metal salts","volume":"12","author":"Fisher T","year":"1985","journal-title":"Contact Dermatitis"},{"key":"ref11","series-title":"Rook's Textbook of Dermatology","first-page":"20.1","volume-title":"Contact dermatitis: allergic","author":"Beck MH","year":"2004","edition":"7th ed"},{"key":"ref12","series-title":"Contact Dermatitis","first-page":"545","volume-title":"Allergens from the European Baseline Series","author":"Andersen KE","year":"2011"},{"key":"ref13","first-page":"149","article-title":"Study on lymphocyte proliferation in nickel-sensitive patients","volume":"80","author":"Sanchez APG","year":"2005","journal-title":"An Bras Dermatol"},{"key":"ref14","article-title":"IL-13: a marker of chromium contact allergy","author":"Martins LEAMM","year":"2012","journal-title":"J EurAcadDermatolVenereol"},{"key":"ref15","series-title":"Samter's immunologic diseases","author":"Frank MM","year":"1995","edition":"5th ed"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1111\/j.1365-2249.2006.03226.x","article-title":"Nickel, cobalt, chromium, palladium and gold induced a mixed Th1and Th2 type cytokine response in vitro in subjects with contact allergy to the respective metals","volume":"146","author":"Minang JT","year":"2006","journal-title":"Clin Exp Immunol"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"1468","DOI":"10.1111\/j.1365-2222.2008.02970.x","article-title":"Detection of chromium allergy by cellular in vitro methods","volume":"38","author":"Lindemann M","year":"2008","journal-title":"Clin Exp Allergy"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1034\/j.1600-0536.2003.00067.x","article-title":"Use of in vitro release of interferon gama in the diagnosis of contact allergy to potassium dichromate a controlled study","volume":"48","author":"Trattner A","year":"2003","journal-title":"Contact Dermatitis"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"634","DOI":"10.1111\/j.0906-6705.2005.00344.x","article-title":"Improved detection of allergen- specific T-cell responses in allergic contact dermatitis through the addition of \"cytokine cocktails\"","volume":"14","author":"Moed H","year":"2005","journal-title":"Exp Dermatol"},{"key":"ref20","first-page":"70","article-title":"The lipophilic haptenparthenolide induces interferon-\u00ce\u00b3 and interleukin-13 production by peripheral blood-derived CD8+ T cells from contact allergic subjects in vitro","volume":"158","author":"Wahlkvist H","year":"2008","journal-title":"Br J Dermatol"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"687","DOI":"10.1016\/S0190-9622(99)70002-2","article-title":"A noninvasive method for quantifying and distinguishing inflammatory skin reactions","volume":"41","author":"Morhenn VB","year":"1999","journal-title":"J Am Acad Dermatol"}],"container-title":["Anais Brasileiros de Dermatologia"],"original-title":[],"link":[{"URL":"http:\/\/www.scielo.br\/pdf\/abd\/v88n5\/0365-0596-abd-88-05-0719.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,5,19]],"date-time":"2025-05-19T15:00:42Z","timestamp":1747666842000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_arttext&pid=S0365-05962013000500719&lng=en&tlng=en"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,10]]},"references-count":21,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2013,10]]}},"alternative-id":["S0365-05962013000500719"],"URL":"https:\/\/doi.org\/10.1590\/abd1806-4841.20132164","relation":{},"ISSN":["0365-0596"],"issn-type":[{"value":"0365-0596","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,10]]}}}