{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T00:32:17Z","timestamp":1789173137787,"version":"build-2803163510"},"reference-count":32,"publisher":"FapUNIFESP (SciELO)","license":[{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Innovation and Entrepreneurship Training Program for College Students in Sichuan Province","award":["S202110634018"],"award-info":[{"award-number":["S202110634018"]}]},{"name":"Innovation and Entrepreneurship Training Program for College Students in Sichuan Province","award":["S202210634177"],"award-info":[{"award-number":["S202210634177"]}]},{"name":"Innovation and Entrepreneurship Training Program for College Students in Sichuan Province","award":["S202310634047"],"award-info":[{"award-number":["S202310634047"]}]},{"name":"Innovation and Entrepreneurship Training Program for College Students in Sichuan Province","award":["CBY19-QD07"],"award-info":[{"award-number":["CBY19-QD07"]}]},{"name":"Doctor Initiation Fund of North Sichuan Medical College","award":["S202110634018"],"award-info":[{"award-number":["S202110634018"]}]},{"name":"Doctor Initiation Fund of North Sichuan Medical College","award":["S202210634177"],"award-info":[{"award-number":["S202210634177"]}]},{"name":"Doctor Initiation Fund of North Sichuan Medical College","award":["S202310634047"],"award-info":[{"award-number":["S202310634047"]}]},{"name":"Doctor Initiation Fund of North Sichuan Medical College","award":["CBY19-QD07"],"award-info":[{"award-number":["CBY19-QD07"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acta Cir. Bras."],"abstract":"<jats:p>ABSTRACT  Purpose: To explore the protective effects of borneol in myocardial ischemia-reperfusion injury (MIRI) and the mechanism of apoptosis.  Methods: Cell viability was detected by CCK-8. The total superoxide dismutase (T-SOD) and lactate dehydrogenase (LDH) leakage of cells were tested by biochemical assay kit. Detection of apoptosis was by flow cytometry. Serum levels of creatine kinase isoenzyme MB (CK-MB), LDH, and cardiac troponin I (cTnI) were detected by enzyme-linked immunosorbent assay. Myocardial infarction area and pathological changes were observed via 2,3,5-triphenyltetrazolium chloride (TTC) staining and hematoxylin and eosin staining. The expressions of apoptosis-related proteins in cells and myocardial tissues were detected by Western blot.  Results: H9c2 cell viability was significantly increased by pretreatment with 16 and 32 \u03bcg\/mL of borneol. Borneol pretreatment significantly increased the T-SOD levels and reduced LDH leakage and apoptosis. In MIRI rats, borneol pretreatment significantly reduced serum levels of CK-MB, LDH and cTnI, decreased myocardial infarction area, and improved myocardial injury in different degree. Western blot results showed that borneol pretreatment significantly reduced the expression of Bcl-2-associated X protein (Bax) and Cysteine-aspartate protease-3 (Caspase-3) in cells and myocardial tissues of rats.  Conclusion: Borneol can protect myocardial injury cells and mitigate MIRI by inhibiting cardiomyocyte apoptosis.<\/jats:p>","DOI":"10.1590\/acb402225","type":"journal-article","created":{"date-parts":[[2025,3,11]],"date-time":"2025-03-11T22:45:04Z","timestamp":1741733104000},"source":"Crossref","is-referenced-by-count":2,"title":["Effects of borneol on apoptosis of hypoxia\/reoxygenation H9c2 cells and myocardial ischemia-reperfusion injury rats"],"prefix":"10.1590","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-3876-6546","authenticated-orcid":false,"given":"Hui","family":"Zhang","sequence":"first","affiliation":[{"name":"North Sichuan Medical College,  China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-9106-0851","authenticated-orcid":false,"given":"Junfang","family":"Dong","sequence":"additional","affiliation":[{"name":"North Sichuan Medical College,  China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9689-5448","authenticated-orcid":false,"given":"Jianwu","family":"Zhang","sequence":"additional","affiliation":[{"name":"North Sichuan Medical College,  China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-4613-225X","authenticated-orcid":false,"given":"Hongxue","family":"Chen","sequence":"additional","affiliation":[{"name":"North Sichuan Medical College,  China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-7221-6923","authenticated-orcid":false,"given":"Ting","family":"Liu","sequence":"additional","affiliation":[{"name":"North Sichuan Medical College,  China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-3999-8564","authenticated-orcid":false,"given":"Ruixue","family":"Gan","sequence":"additional","affiliation":[{"name":"North Sichuan Medical College,  China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6980-9105","authenticated-orcid":false,"given":"Jing","family":"Wen","sequence":"additional","affiliation":[{"name":"North Sichuan Medical College,  China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3574-7986","authenticated-orcid":false,"given":"Yangyou","family":"Li","sequence":"additional","affiliation":[{"name":"North Sichuan Medical College,  China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"530","published-online":{"date-parts":[[2025]]},"reference":[{"issue":"1","key":"ref1","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1016\/j.mayocp.2019.05.001","article-title":"Acute myocardial infarction in young individuals","volume":"95","author":"Gulati R","year":"2020","journal-title":"Mayo Clin Proc"},{"issue":"20","key":"ref2","doi-asserted-by":"crossref","first-page":"3271","DOI":"10.3390\/cells11203271","article-title":"Research progress on the role of pyroptosis in myocardial ischemia-reperfusion injury","volume":"11","author":"Liu Y","year":"2022","journal-title":"Cells"},{"issue":"3","key":"ref3","doi-asserted-by":"crossref","first-page":"486","DOI":"10.1038\/s41418-017-0012-4","article-title":"Molecular mechanisms of cell death: recommendations of the Nomenclature Committee on Cell Death 2018","volume":"25","author":"Galluzzi L","year":"2018","journal-title":"Cell Death Differ"},{"issue":"5","key":"ref4","doi-asserted-by":"crossref","first-page":"1106","DOI":"10.1038\/s41423-020-00630-3","article-title":"Necroptosis, pyroptosis and apoptosis: an intricate game of cell death","volume":"18","author":"Bertheloot D","year":"2021","journal-title":"Cell Mol Immunol"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"6614009","DOI":"10.1155\/2021\/6614009","article-title":"Role of oxidative stress in reperfusion following myocardial ischemia and its treatments","volume":"2021","author":"Xiang M","year":"2021","journal-title":"Oxid Med Cell Longev"},{"key":"ref6","series-title":"Pharmacopoeia of People\u2019s Republic of China. 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cell death pathways in hearing loss: A review of apoptosis, autophagy and programmed necrosis","volume":"53","author":"Wu J","year":"2020","journal-title":"Cell Prolif"},{"key":"ref30","first-page":"9626703","article-title":"Pretreatment with panaxatriol saponin attenuates mitochondrial apoptosis and oxidative stress to facilitate treatment of myocardial ischemia-reperfusion injury via the regulation of Keap1\/Nrf2 activity","volume":"2022","author":"Yao H","year":"2022","journal-title":"Oxid Med Cell Longev"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"922484","DOI":"10.3389\/fphar.2022.922484","article-title":"Sinomenine confers protection against myocardial ischemia reperfusion injury by preventing oxidative stress, cellular apoptosis, and inflammation","volume":"13","author":"Xia B","year":"2022","journal-title":"Front Pharmacol"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"924174","DOI":"10.3389\/fphar.2022.924174","article-title":"Indole-3-carbinol 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