{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T15:15:41Z","timestamp":1761578141988,"version":"build-2065373602"},"reference-count":49,"publisher":"FapUNIFESP (SciELO)","license":[{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Inner Mongolia Autonomous Region Health Science and Technology","award":["02201468"],"award-info":[{"award-number":["02201468"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acta Cir. Bras."],"abstract":"<jats:p>ABSTRACT  Purpose: To scrutinize the protective effect of alpinumisoflavone against the acute reflux esophagitis (RE) in the rats and to explore the underlying mechanism.  Methods: RAW 264.7 cells were used for in-vitro study, and MTT assay was used to access the cell viability. The cells were treated with lipopolysaccharide (LPS) and estimation the inflammatory cytokines and parameters. A surgical procedure was performed for the induction of RE followed by the oral administration of alpinumisoflavone (5, 10 and 15 mg\/kg). The esophagitis lesion score, gross esophageal score, damage ratio, pH and gastric volume, NO level, alcian blue, H2O2, free iron, calcium, antioxidant, inflammatory cytokines and inflammatory parameters were estimated.  Results: Alpinumisoflavone treatment significantly (p &lt; 0.001) suppressed cell viability and NO levels, along with a reduction in inflammatory cytokines like tumor necrosis factor (TNF)-\u03b1, interleukin (IL)-1\u03b2, IL-6 and inflammatory parameters such as cyclooxygenase-2 (COX-2), prostaglandin E22 (PGE2), and inducible nitric oxide synthase (iNOS) against the LPS treatment. Alpinumisoflavone treated group rats suppressed the esophagitis lesion score, gross esophageal score damage ratio, and gastric volume and improved the pH level. Alpinumisoflavone treatment significantly (p &lt; 0.001) suppressed the level of NO level, alcian blue, H2O2, free iron and calcium. Alpinumisoflavone significantly (p &lt; 0.001) altered the level of antioxidant parameters such as malondialdehyde, superoxide dismutase, glutathione, glutathione peroxidase, catalase; inflammatory cytokines viz., TNF-\u03b1, IL-6, IL-1\u03b2, IL-10, IL-18; and inflammatory parameters including COX-2, NF-\u03baB, and PGE2.  Conclusion: Alpinumisoflavone ameliorates the acute reflux esophagitis via suppression of inflammatory parameters.<\/jats:p>","DOI":"10.1590\/acb405625","type":"journal-article","created":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T15:02:42Z","timestamp":1761577362000},"source":"Crossref","is-referenced-by-count":0,"title":["Alpinumisoflavone ameliorates experimental acute reflux esophagitis in rats via regulation of inflammatory pathway"],"prefix":"10.1590","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-5108-9354","authenticated-orcid":false,"family":"Zhaorigetu","sequence":"first","affiliation":[{"name":"The Affiliated Hospital of Inner Mongolia University for Nationalities,  China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-9093-7090","authenticated-orcid":false,"given":"Zi","family":"Ge","sequence":"additional","affiliation":[{"name":"Baoshan branch of Huashan Hospital Affiliated to Fudan University,  China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-7042-2225","authenticated-orcid":false,"given":"Wei","family":"Zhang","sequence":"additional","affiliation":[{"name":"Shaanxi Province Hospital of Traditional Chinese Medicine,  China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","published-online":{"date-parts":[[2025]]},"reference":[{"issue":"2","key":"ref1","first-page":"49","article-title":"Protective effect of Fenugreek (Trigonella foenum graecum) seed extract on experimental reflux esophagitis in rat","volume":"8","author":"Kheirandish R","year":"2013","journal-title":"Comp Clin Pathol"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1186\/s13765-019-0412-x","article-title":"Geraniin ameliorate experimental acute reflux esophagitis via NF-\u03baB regulated anti-inflammatory activities in rats","volume":"62","author":"Nam HH","year":"2019","journal-title":"Appl Biol Chem"},{"issue":"3","key":"ref3","doi-asserted-by":"crossref","first-page":"321","DOI":"10.4163\/jnh.2021.54.3.321","article-title":"Effects of a mixture of Citri Pericarpium and Scutellariae Radix on acute reflux esophagitis in rats","volume":"54","author":"Lee JA","year":"2021","journal-title":"J Nutr Health"},{"key":"ref4","article-title":"Protective Effects of inflammation of curcumae longae rhizoma 30% EtOH extract on acute reflux esophagitis rats","volume":"2021","author":"Lee JA","year":"2021","journal-title":"BioMed Res Int"},{"issue":"17","key":"ref5","doi-asserted-by":"crossref","first-page":"2427","DOI":"10.2169\/internalmedicine.2535-18","article-title":"Vonoprazan versus lansoprazole for the initial treatment of reflux esophagitis: a cost-effectiveness analysis in Japan","volume":"58","author":"Habu Y","year":"2019","journal-title":"Internal Med"},{"issue":"3","key":"ref6","first-page":"545","article-title":"Rutaecarpine regulates the expression of pro-inflammatory cytokines to induce protective effects in the murine model of acute reflux esophagitis","volume":"43","author":"Cui W","year":"2023","journal-title":"Pak Vet J"},{"issue":"10","key":"ref7","doi-asserted-by":"crossref","first-page":"2500","DOI":"10.3390\/foods10102500","article-title":"Anti-inflammation and protective effects of anethum graveolens l. 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