{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,4,2]],"date-time":"2022-04-02T10:44:36Z","timestamp":1648896276326},"reference-count":6,"publisher":"FapUNIFESP (SciELO)","issue":"8","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arq Bras Endocrinol Metab"],"published-print":{"date-parts":[[2011,11]]},"abstract":"<jats:p>INTRODUCTION: Androgen actions are exerted upon the androgen receptor (AR), and complete genital virilization of normal 46,XY individuals depends on adequate function and expression of the AR gene in a tissue-specific manner. OBJECTIVE: Standardization of normal ARmRNA in androgen-sensitive tissues. MATERIALS AND METHODS: In this study, we determined the quantitative amounts of ARmRNA in peripheral blood mononuclear, urethral mucosa and preputial skin cells of control subjects with phimosis by using RT-PCR. RESULTS: The mean (SD) values of AR expression in blood, urethra and prepuce were: 0.01 (0.01); 0.43 (0.32); 0.31 (0.36), respectively. CONCLUSION: The AR expression is low in blood and equivalent in urethral mucosa and preputial skin, which may be useful in the diagnosis of individuals with abnormal external genitalia.<\/jats:p>","DOI":"10.1590\/s0004-27302011000800024","type":"journal-article","created":{"date-parts":[[2012,1,4]],"date-time":"2012-01-04T02:56:05Z","timestamp":1325645765000},"page":"665-668","source":"Crossref","is-referenced-by-count":1,"title":["Absolute measurement of androgen receptor mRNA in peripheral blood mononuclear, preputial skin and urethral mucosa cells of control individuals with phimosis using qRT-PCR"],"prefix":"10.1590","volume":"55","author":[{"given":"Tatiane Sousa e","family":"Silva","sequence":"first","affiliation":[{"name":"Santa Casa de S\u00e3o Paulo,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Flavio","family":"Richetti","sequence":"additional","affiliation":[{"name":"Santa Casa de S\u00e3o Paulo,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniela Patricia Palmeira Santos","family":"Cunha","sequence":"additional","affiliation":[{"name":"Santa Casa de Miseric\u00f3rdia,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonio Carlos Moreira","family":"Amarante","sequence":"additional","affiliation":[{"name":"Hospital Pequeno Pr\u00edncipe,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jovelino Quintino de Souza","family":"Le\u00e3o","sequence":"additional","affiliation":[{"name":"Hospital Darcy Vargas,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlos Alberto","family":"Longui","sequence":"additional","affiliation":[{"name":"Santa Casa de S\u00e3o Paulo,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","reference":[{"issue":"1","key":"ref1","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1038\/35094009","article-title":"The development of androgen-independent prostate cancer","volume":"1","author":"Feldman BJ","year":"2001","journal-title":"Nat Rev Cancer"},{"issue":"1","key":"ref2","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1590\/S0004-27302005000100012","article-title":"Androgen insensitivity syndrome: clinical, hormonal and molecular analysis of 33 cases","volume":"49","author":"Melo KF","year":"2005","journal-title":"Arq Bras Endocrinol Metabol","ISSN":"http:\/\/id.crossref.org\/issn\/0004-2730","issn-type":"print"},{"issue":"4","key":"ref3","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1016\/0039-128X(96)00008-6","article-title":"Molecular basis of androgen insensitivity","volume":"61","author":"Brinkmann A","year":"1996","journal-title":"Steroids","ISSN":"http:\/\/id.crossref.org\/issn\/0039-128X","issn-type":"print"},{"key":"ref4","series-title":"Menino ou menina? Dist\u00farbios da diferencia\u00e7\u00e3o do sexo","first-page":"251","volume-title":"Insensibilidade androg\u00eanica","author":"Guerra G Jr","year":"2010","edition":"Segunda"},{"issue":"4","key":"ref5","doi-asserted-by":"crossref","first-page":"1112","DOI":"10.1093\/humrep\/17.4.1112","article-title":"A high hypospadias rate in The Netherlands","volume":"17","author":"Pierik FH","year":"2002","journal-title":"Hum Reprod","ISSN":"http:\/\/id.crossref.org\/issn\/0268-1161","issn-type":"print"},{"issue":"8","key":"ref6","doi-asserted-by":"crossref","first-page":"651","DOI":"10.1007\/s00109-006-0049-1","article-title":"Tissue-specific transcription profiles of sex steroid biosynthesis enzymes and the androgen receptor","volume":"84","author":"Hoppe U","year":"2006","journal-title":"J Mol Med","ISSN":"http:\/\/id.crossref.org\/issn\/0377-046X","issn-type":"print"}],"container-title":["Arquivos Brasileiros de Endocrinologia &amp; Metabologia"],"original-title":[],"link":[{"URL":"http:\/\/www.scielo.br\/pdf\/abem\/v55n8\/24.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2017,5,26]],"date-time":"2017-05-26T23:29:23Z","timestamp":1495841363000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_arttext&pid=S0004-27302011000800024&lng=en&tlng=en"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,11]]},"references-count":6,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2011,11]]}},"alternative-id":["S0004-27302011000800024"],"URL":"https:\/\/doi.org\/10.1590\/s0004-27302011000800024","relation":{},"ISSN":["0004-2730"],"issn-type":[{"value":"0004-2730","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,11]]}}}