{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,4]],"date-time":"2025-10-04T01:31:04Z","timestamp":1759541464041},"reference-count":30,"publisher":"FapUNIFESP (SciELO)","issue":"9","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arq Bras Endocrinol Metab"],"published-print":{"date-parts":[[2012,12]]},"abstract":"<jats:p>OBJECTIVE: Adequate isolation of nucleic acids from peripheral blood, fine-needle aspiration cells in stained slides, and fresh and formalin-fixed\/paraffin-embedded tissues is crucial to ensure the success of molecular endocrinology techniques, especially when samples are stored for long periods, or when no other samples can be collected from patients who are lost to follow-up. Here, we evaluate several procedures to improve current methodologies for DNA (salting-out) and RNA isolation. MATERIALS AND METHODS: We used proteinase K treatment, heat shock, and other adaptations to increase the amount and quality of the material retrieved from the samples. RESULTS: We successfully isolated DNA and RNA from the samples described above, and this material was suitable for PCR, methylation profiling, real-time PCR and DNA sequencing. CONCLUSION: The techniques herein applied to isolate nucleic acids allowed further reliable molecular analyses. Arq Bras Endocrinol Metab. 2012;56(9):618-26<\/jats:p>","DOI":"10.1590\/s0004-27302012000900004","type":"journal-article","created":{"date-parts":[[2013,1,16]],"date-time":"2013-01-16T08:25:43Z","timestamp":1358324743000},"page":"618-626","source":"Crossref","is-referenced-by-count":32,"title":["Optimizing nucleic acid extraction from thyroid fine-needle aspiration cells in stained slides, formalin-fixed\/paraffin-embedded tissues, and long-term stored blood samples"],"prefix":"10.1590","volume":"56","author":[{"given":"Marina M. L.","family":"Kizys","sequence":"first","affiliation":[{"name":"Universidade Federal de Sao Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mirian G.","family":"Cardoso","sequence":"additional","affiliation":[{"name":"Universidade Federal de Sao Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susan C.","family":"Lindsey","sequence":"additional","affiliation":[{"name":"Universidade Federal de Sao Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michelle Y.","family":"Harada","sequence":"additional","affiliation":[{"name":"Universidade Federal de Sao Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fernando A.","family":"Soares","sequence":"additional","affiliation":[{"name":"Universidade Federal de Sao Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Clara C.","family":"Melo","sequence":"additional","affiliation":[{"name":"Universidade Federal de Sao Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marlyn Z.","family":"Montoya","sequence":"additional","affiliation":[{"name":"Universidade Federal de Sao Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Teresa S.","family":"Kasamatsu","sequence":"additional","affiliation":[{"name":"Universidade Federal de Sao Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ilda S.","family":"Kunii","sequence":"additional","affiliation":[{"name":"Universidade Federal de Sao Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gisele","family":"Giannocco","sequence":"additional","affiliation":[{"name":"Universidade Federal de Sao Paulo, Brazil; Faculdade de Medicina do ABC, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jo\u00e3o Roberto M.","family":"Martins","sequence":"additional","affiliation":[{"name":"Universidade Federal de Sao Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Janete M.","family":"Cerutti","sequence":"additional","affiliation":[{"name":"Universidade Federal de Sao Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rui M. B.","family":"Maciel","sequence":"additional","affiliation":[{"name":"Universidade Federal de Sao Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Magnus R.","family":"Dias-da-Silva","sequence":"additional","affiliation":[{"name":"Universidade Federal de Sao Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","reference":[{"issue":"4","key":"ref1","doi-asserted-by":"crossref","first-page":"973","DOI":"10.1158\/1055-9965.EPI-10-0091","article-title":"Simultaneous recovery of DNA and RNA from formalin-fixed paraffin-embedded tissue and application in epidemiologic studies","volume":"19","author":"Huang WY","year":"2010","journal-title":"Cancer epidemiology, biomarkers & prevention: a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology."},{"issue":"49","key":"ref2","doi-asserted-by":"crossref","DOI":"10.3791\/2763","article-title":"DNA extraction from paraffin 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M","year":"1993","journal-title":"J Virol Methods."},{"issue":"1","key":"ref21","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1016\/0003-2697(87)90021-2","article-title":"Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction","volume":"162","author":"Chomczynski P","year":"1987","journal-title":"Anal Biochem."},{"issue":"2","key":"ref22","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/j.prp.2007.09.002","article-title":"RNA isolation and quantitative PCR from HOPE- and formalin-fixed bovine lymph node tissues","volume":"204","author":"Witchell J","year":"2008","journal-title":"Pathol Res Pract."},{"issue":"4","key":"ref23","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1006\/meth.2001.1263","article-title":"Real-time PCR analysis of DNA and RNA extracted from formalin-fixed and paraffin-embedded biopsies","volume":"25","author":"Lehmann 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by the polymerase chain reaction","volume":"72","author":"de Roda Husman AM","year":"1995","journal-title":"Br J Cancer."},{"issue":"11","key":"ref30","doi-asserted-by":"crossref","first-page":"847","DOI":"10.1038\/nrg1707","article-title":"Genotyping errors: causes, consequences and solutions","volume":"6","author":"Pompanon F","year":"2005","journal-title":"Nat Rev Genet."}],"container-title":["Arquivos Brasileiros de Endocrinologia &amp; 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