{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,3]],"date-time":"2026-03-03T12:29:31Z","timestamp":1772540971269,"version":"3.50.1"},"reference-count":28,"publisher":"FapUNIFESP (SciELO)","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arq. Gastroenterol."],"abstract":"<jats:p>ABSTRACT  BACKGROUND: Mast cells exert a substantial role in gastrointestinal allergic diseases. Therefore, it is reasonable to presume that mast cell may aid diagnosis in eosinophilic gastroenteropathy.  OBJECTIVE: To evaluate whether mast cell count in the esophageal epithelium can discriminate eosinophilic esophagitis, proton-pump inhibitor (PPI)-responsive eosinophilic esophagitis and gastroesophageal reflux esophagitis.  METHODS:  Retrospectively we reviewed the files of 53 consecutive patients (age: 7.8 years; range: 8-14 years) with definitive diagnose established during clinical follow up in a universitary outpatient clinic as follow: eosinophilic esophagitis (N=23), PPI-responsive eosinophilic esophagitis (N=15) and gastroesophageal reflux esophagitis (N=15). Eosinophil count in the esophageal epithelium in slides stained with H-E was reviewed and immunohistochemistry for mast cell tryptase was performed.  RESULTS:  Count of eosinophils\/high-power field (HPF) higher than 15 were found in 14 out of 15 reflux esophagitis patients. The mean count of eosinophils\/HPF was similar in eosinophilic esophagitis patients and in those with PPI-responsive eosinophilic esophagitis (42 and 39 eosinophils\/HPF, respectively, P=0.47). Values of mast cell tryptase (+) were higher in eosinophilic esophagitis [median: 25 mast cells\/HPF; range (17-43) ] and in PPI-responsive eosinophilic esophagitis patients [25 (16-32) ], compared to reflux esophagitis [4 (2-14) ], P&lt;0.001. There was no difference between the mean count of mast cells\/HPF in the esophageal epithelium of eosinophilic esophagitis patients and PPI-responsive eosinophilic esophagitis patients, respectively, 26 and 24 mast cells\/HPF, P=0.391.  CONCLUSION: Tryptase staining of mast cells differentiates eosinophilic esophagitis from reflux esophagitis.<\/jats:p>","DOI":"10.1590\/s0004-2803.201700000-23","type":"journal-article","created":{"date-parts":[[2017,5,11]],"date-time":"2017-05-11T12:55:11Z","timestamp":1494507311000},"page":"192-196","source":"Crossref","is-referenced-by-count":10,"title":["MAST CELLS DISTINGUISH EOSINOPHILIC ESOPHAGITIS IN PEDIATRIC PATIENTS"],"prefix":"10.1590","volume":"54","author":[{"given":"Elizete Aparecida","family":"LOMAZI","sequence":"first","affiliation":[{"name":"Universidade Estadual de Campinas,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nelson Ary","family":"BRANDALISE","sequence":"additional","affiliation":[{"name":"Universidade Estadual de Campinas,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria de F\u00e1tima Pimenta Correa","family":"SERVIDONI","sequence":"additional","affiliation":[{"name":"Universidade Estadual de Campinas,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvia Regina","family":"CARDOSO","sequence":"additional","affiliation":[{"name":"Universidade Estadual de Campinas,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luciana Rodrigues","family":"MEIRELLES","sequence":"additional","affiliation":[{"name":"Universidade Estadual de Campinas,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","published-online":{"date-parts":[[2017,5,8]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"536","DOI":"10.1172\/JCI26679","article-title":"Eotaxin-3 and a uniquely conserved gene-expression profile in eosinophilic esophagitis","volume":"116","author":"Blanchard C","year":"2006","journal-title":"J Clin Invest"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"679","DOI":"10.1038\/ajg.2013.71","article-title":"ACG clinical guideline: evidenced based approach to the diagnosis and management of esophageal eosinophilia and eosinophilic esophagitis (EoE)","volume":"108","author":"Dellon ES","year":"2013","journal-title":"Am J Gastroenterol"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1016\/j.jpeds.2008.07.042","article-title":"Predictors of response to proton pump inhibitor therapy among children with significant esophageal eosinophilia","volume":"154","author":"Dranove JE","year":"2009","journal-title":"J Pediatr"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"2015","DOI":"10.1016\/j.cgh.2014.06.019","article-title":"Markers of eosinophilic inflammation for diagnosis of eosinophilic esophagitis and proton pump inhibitor-responsive esophageal eosinophilia: a prospective study","volume":"12","author":"Evan S","year":"2014","journal-title":"Clin Gastroenterol Hepatol"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"300","DOI":"10.1111\/j.1365-2036.2011.04922.x","article-title":"Results of ambulatory pH monitoring do not reliably predict response to therapy in patients with eosinophilic oesophagitis","volume":"35","author":"Francis DL","year":"2012","journal-title":"Aliment Pharmacol Ther"},{"key":"ref6","first-page":"141","article-title":"[Eosinophilic esophagitis in 29 pediatric patients]","volume":"45","author":"Ferreira CT","year":"2008","journal-title":"Arq. 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