{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T05:25:46Z","timestamp":1735709146794,"version":"3.32.0"},"reference-count":31,"publisher":"FapUNIFESP (SciELO)","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arq. Gastroenterol."],"published-print":{"date-parts":[[2003,9]]},"abstract":"<jats:p>RACIONAL: Laminina s\u00e9rica tem sido correlacionada com a hipertens\u00e3o portal e a capilariza\u00e7\u00e3o dos sinus\u00f3ides na doen\u00e7a hep\u00e1tica cr\u00f4nica. Pouco \u00e9 conhecido sobre sua din\u00e2mica nas doen\u00e7as hep\u00e1ticas. OBJETIVO: Estudar os n\u00edveis s\u00e9ricos de laminina e correlacion\u00e1-los com graus de fibrose hep\u00e1tica e hipertens\u00e3o portal em ratos tratados com tetracloreto de carbono. MATERIAL E M\u00c9TODO: Quarenta e nove ratos albinos Wistar foram mantidos como controles (n = 16) ou tratados com solu\u00e7\u00e3o de tetracloreto de carbono a 8% (n = 23). Entre a 6\u00aa e 16\u00aa semana de experimento animais foram sacrificados ap\u00f3s cateteriza\u00e7\u00e3o da veia porta e medida da press\u00e3o portal. Fragmentos de f\u00edgado foram fixados em formol para microscopia \u00f3ptica. A fibrose hep\u00e1tica foi classificada em perivenular, septal incompleta e completa e cirrose. Concentra\u00e7\u00e3o de laminina circulante foi determinada pela t\u00e9cnica de ensaio imunoenzim\u00e1tico com anticorpo desenvolvido a partir de laminina isolada de tumor Engelbreth-Holm-Swarm e purificado em coluna de afinidade. RESULTADOS: A press\u00e3o portal correlacionou-se com grau de fibrose hep\u00e1tica (r = 0,82; n = 45) e seus n\u00edveis nos grupos fibrose septal (10,8 \u00b1 1,2 cm H2O) e cirrose (13,6 + 3,1 cm H2O) foram estatisticamente superiores aos observados no grupo-controle (7,9 + 1,5 cmH2O) e no grupo com fibrose perivenular (9,1 + 0,8 cmH2O). A concentra\u00e7\u00e3o de laminina no sangue perif\u00e9rico dos cirr\u00f3ticos (40,0 + 18,7 \u00b5g\/dL) foi significativamente superior aos grupos-controle (13,8 + 12,1 \u00b5g\/dL), fibrose perivenular (19,1 + 15,5) e fibrose septal (22,2 + 27,0 \u00b5g\/dL). Laminina circulante correlacionou-se de maneira significativa com o grau de fibrose hep\u00e1tica (r = 0,59; n = 49) e com os n\u00edveis de press\u00e3o portal (r = 0,29; n = 45). CONCLUS\u00d5ES: Na intoxica\u00e7\u00e3o cr\u00f4nica experimental pelo tetracloreto de carbono, n\u00edveis circulantes de laminina correlacionam-se mais com o grau de fibrose hep\u00e1tica do que com os n\u00edveis de hipertens\u00e3o portal.<\/jats:p>","DOI":"10.1590\/s0004-28032003000300007","type":"journal-article","created":{"date-parts":[[2005,4,15]],"date-time":"2005-04-15T14:20:55Z","timestamp":1113574855000},"page":"173-176","source":"Crossref","is-referenced-by-count":2,"title":["Eleva\u00e7\u00e3o dos n\u00edveis s\u00e9ricos de laminina na cirrose hep\u00e1tica induzida pelo tetracloreto de carbono"],"prefix":"10.1590","volume":"40","author":[{"given":"Lindalva Batista","family":"Neves","sequence":"first","affiliation":[{"name":"Universidade Federal de S\u00e3o Paulo"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Regina Maria","family":"Catarino","sequence":"additional","affiliation":[{"name":"Universidade Federal de S\u00e3o Paulo"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Regina Regis","family":"Silva","sequence":"additional","affiliation":[{"name":"Universidade Federal de S\u00e3o Paulo"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edison Roberto","family":"Parise","sequence":"additional","affiliation":[{"name":"Universidade Federal de S\u00e3o Paulo"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","reference":[{"key":"ref1","first-page":"1200","article-title":"Serum laminin P1 levels do not reflect critically elevated portal pressure in patients with liver cirrhosis","volume":"44","author":"Bahr MJ","year":"1997","journal-title":"Hepatogastroenterology","ISSN":"https:\/\/id.crossref.org\/issn\/0172-6390","issn-type":"print"},{"year":"1987","series-title":"Redu\u00e7\u00e3o da fibrose hep\u00e1tica induzida pelo tetracloreto de carbono, pela administra\u00e7\u00e3o pr\u00e9via de vitamina A [disserta\u00e7\u00e3o]","author":"Chehter L","key":"ref2"},{"key":"ref3","series-title":"Primer of biostatistics","first-page":"473","author":"Glantz SA","year":"1997","edition":"4"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1016\/0009-8981(86)90008-2","article-title":"Serum concentration of laminin and aminoterminal propeptide of type III procollagen and in relation to the portal venous pressure of fibrotic liver diseases","volume":"161","author":"Gressner AM","year":"1986","journal-title":"Clin Chim Acta","ISSN":"https:\/\/id.crossref.org\/issn\/0009-8981","issn-type":"print"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"1240","DOI":"10.1007\/BF01734467","article-title":"Serum laminin: Its concentration increases with portal hypertension in cirrhotic liver disease","volume":"64","author":"Gressner AM","year":"1986","journal-title":"Klin Wochensch"},{"key":"ref6","first-page":"95","article-title":"The predictive value of serum laminin for portal hypertension in chronic liver disease","volume":"35","author":"Gressner AM","year":"1988","journal-title":"Hepatogastroenterology","ISSN":"https:\/\/id.crossref.org\/issn\/0172-6390","issn-type":"print"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1136\/gut.21.1.63","article-title":"Distribution of basement membrane proteins in normal and fibrotic human liver: collagen type IV, laminin and fibronectin","volume":"21","author":"Hahn EG","year":"1980","journal-title":"Gut","ISSN":"https:\/\/id.crossref.org\/issn\/0017-5749","issn-type":"print"},{"key":"ref8","first-page":"542","article-title":"The predictive value of serum laminin for the risk of variceal bleeding related to portal pressure levels","volume":"42","author":"Kondo M","year":"1995","journal-title":"Hepatogastroenterology","ISSN":"https:\/\/id.crossref.org\/issn\/0172-6390","issn-type":"print"},{"key":"ref9","first-page":"841","article-title":"Laminine s\u00e9rique et pression portale au cours de la cirrhose alcoolique: Une etude chez 39 malades","volume":"12","author":"Mal F","year":"1988","journal-title":"Gastroenterol Clin Biol","ISSN":"https:\/\/id.crossref.org\/issn\/0399-8320","issn-type":"print"},{"key":"ref10","first-page":"57","article-title":"The hepatic extracellular matrix: I. 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