{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,6,29]],"date-time":"2022-06-29T13:54:02Z","timestamp":1656510842951},"reference-count":51,"publisher":"FapUNIFESP (SciELO)","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arq. Gastroenterol."],"published-print":{"date-parts":[[2006,12]]},"abstract":"<jats:p>BACKGROUD: There is substantial evidence that infection with Helicobacter pylori plays a role in the development of gastric cancer and that it is rarely found in gastric biopsy of atrophic gastritis and gastric cancer. On advanced gastric tumors, the bacteria can be lost from the stomach. AIMS: To analyze the hypothesis that the prevalence of H.pylori in operated advanced gastric carcinomas and adjacent non-tumor tissues is high, comparing intestinal and diffuse tumors according to Lauren's classification METHODS: A prospective controlled study enrolled 56 patients from \"Hospital Universit\u00e1rio\", Federal University of Rio Grande do Norte, Natal, RN, Brazil, with advanced gastric cancer, treated from February 2000 to March 2003. Immediately after partial gastrectomy, the resected stomach was opened and several mucosal biopsy samples were taken from the gastric tumor and from the adjacent mucosa within 4 cm distance from the tumor margin. Tissue sections were stained with hematoxylin and eosin. Lauren's classification for gastric cancer was used, to analyse the prevalence of H. pylori in intestinal or diffuse carcinomas assessed by the urease rapid test, IgG by ELISA and Giemsa staining. H. pylori infected patients were treated with omeprazole, clarithromycin and amoxicillin for 7 days. Follow-up endoscopy and serology were performed 6 months after treatment to determine successful eradication of H. pylori in non-tumor tissue. Thereafter, follow-up endoscopies were scheduled annually. Chi-square and MacNemar tests with 0.05 significance were used. RESULTS: Thirty-four tumors (60.7%) were intestinal-type and 22 (39.3%) diffuse type carcinomas. In adjacent non-tumor gastric mucosa, chronic gastritis were found in 53 cases (94.6%) and atrophic mucosa in 36 patients (64.3%). All the patients with atrophic mucosa were H. pylori positive. When examined by Giemsa and urease test, H. pylori positive rate in tumor tissue of intestinal type carcinomas was higher than that in diffuse carcinomas. In tumor tissues, 34 (60.7%) H. pylori-positive in gastric carcinomas were detected by Giemsa method. H. pylori was observed in 30 of 56 cases (53.5%) in tissues 4 cm adjacent to tumors. This difference was not significant. Eradication of H. pylori in non-tumor tissue of gastric remnant led to a complete negativity on the 12th postoperative month CONCLUSIONS: The data confirmed the hypothesis of a high prevalence of H. pylori in tumor tissue of gastric advanced carcinomas and in adjacent non-tumor mucosa of operated stomachs. The presence of H. pylori was predominant in the intestinal-type carcinoma.<\/jats:p>","DOI":"10.1590\/s0004-28032006000400009","type":"journal-article","created":{"date-parts":[[2007,4,11]],"date-time":"2007-04-11T13:29:42Z","timestamp":1176298182000},"page":"288-292","source":"Crossref","is-referenced-by-count":9,"title":["Prevalence of Helicobacter pylori infection in advanced gastric carcinoma"],"prefix":"10.1590","volume":"43","author":[{"given":"Irami","family":"Ara\u00fajo-Filho","sequence":"first","affiliation":[{"name":"Federal University of Rio Grande do Norte, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9","family":"Brand\u00e3o-Neto","sequence":"additional","affiliation":[{"name":"Federal University of Rio Grande do Norte, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"La\u00edza Ara\u00fajo Mohana","family":"Pinheiro","sequence":"additional","affiliation":[{"name":"Federal University of Rio Grande do Norte, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u00cdtalo Medeiros","family":"Azevedo","sequence":"additional","affiliation":[{"name":"Federal University of Rio Grande do Norte, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fl\u00e1vio Henrique Miranda Ara\u00fajo","family":"Freire","sequence":"additional","affiliation":[{"name":"Federal University of Rio Grande do Norte, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aldo Cunha","family":"Medeiros","sequence":"additional","affiliation":[{"name":"Federal University of Rio Grande do Norte, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"514","DOI":"10.1053\/j.gastro.2004.06.006","article-title":"Determinants and consequences of different levels of CagA phosphorylation for clinical isolates of Helicobacter pylori","volume":"127","author":"Argent 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