{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,3,31]],"date-time":"2022-03-31T20:49:25Z","timestamp":1648759765175},"reference-count":62,"publisher":"FapUNIFESP (SciELO)","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arq. Gastroenterol."],"published-print":{"date-parts":[[2007,6]]},"abstract":"<jats:p>RACIONAL: Helicobacter pylori \u00e9 hoje aceito como o principal agente etiol\u00f3gico de gastrite em seres humanos e fator de risco para \u00falcera p\u00e9ptica e c\u00e2ncer g\u00e1strico. A evolu\u00e7\u00e3o da infec\u00e7\u00e3o est\u00e1 relacionada a diversos fatores, inclusive bacterianos, como presen\u00e7a do gene cagA e o gen\u00f3tipo vacA s1m1, associados ao desenvolvimento de \u00falcera e adenocarcinoma g\u00e1strico. A t\u00e9cnica de RAPD (\"random amplified polimorphic\") tem sido amplamente utilizada para obten\u00e7\u00e3o de impress\u00f5es digitais de DNA para examinar a similaridade entre linhagens. OBJETIVOS: Avaliar a presen\u00e7a de cagA e alelos do vacA em amostras de H. pylori e associar os achados com a doen\u00e7a apresentada e tamb\u00e9m investigar poss\u00edvel clonicidade entre os fatores de virul\u00eancia e as doen\u00e7as com a impress\u00e3o digital de DNA gerada pelo RAPD-PCR. M\u00c9TODOS: Foram inclu\u00eddas 112 amostras provenientes de pacientes com diferentes laudos endosc\u00f3picos: gastrite (n = 41), esofagite de refluxo (n = 14), \u00falcera g\u00e1strica (n = 19) e \u00falcera duodenal (n = 38). A an\u00e1lise dos fatores de virul\u00eancia da bact\u00e9ria foi feita por PCR e as impress\u00f5es digitais de DNA foram estabelecidas pelo m\u00e9todo de RAPD-PCR. RESULTADOS: Os resultados obtidos indicam que houve uma associa\u00e7\u00e3o significativa entre \u00falcera duodenal e o mosaico vacA s1m1. Analisando-se os padr\u00f5es de bandas geradas pelo RAPD-PCR, sete diferentes dendogramas foram constru\u00eddos e n\u00e3o foi poss\u00edvel detectar associa\u00e7\u00e3o significativa entre os agrupamentos, sugerindo que as amostras n\u00e3o possuem perfil clonal. CONCLUS\u00c3O: Os resultados refor\u00e7am a import\u00e2ncia do gene vacA como um marcador de virul\u00eancia do H. pylori. O RAPD da impress\u00e3o digital de DNA realizado foi incapaz de associar o padr\u00e3o de bandas com as enfermidades e os gen\u00f3tipos de vacA e cagA.<\/jats:p>","DOI":"10.1590\/s0004-28032007000200004","type":"journal-article","created":{"date-parts":[[2007,11,17]],"date-time":"2007-11-17T17:48:16Z","timestamp":1195321696000},"page":"107-112","source":"Crossref","is-referenced-by-count":3,"title":["An\u00e1lise das impress\u00f5es digitais de DNA e de fatores de virul\u00eancia de linhagens de Helicobacter pylori"],"prefix":"10.1590","volume":"44","author":[{"given":"Anita P. O.","family":"Godoy","sequence":"first","affiliation":[{"name":"Universidade S\u00e3o Francisco"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ma\u00edra C. B.","family":"Miranda","sequence":"additional","affiliation":[{"name":"Universidade S\u00e3o Francisco"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luciana C.","family":"Paulino","sequence":"additional","affiliation":[{"name":"Universidade S\u00e3o Francisco"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sergio","family":"Mendon\u00e7a","sequence":"additional","affiliation":[{"name":"Universidade S\u00e3o Francisco"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcelo Lima","family":"Ribeiro","sequence":"additional","affiliation":[{"name":"Universidade S\u00e3o Francisco"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9","family":"Pedrazzoli Jr.","sequence":"additional","affiliation":[{"name":"Universidade S\u00e3o 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