{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,6,24]],"date-time":"2022-06-24T12:47:42Z","timestamp":1656074862432},"reference-count":36,"publisher":"FapUNIFESP (SciELO)","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arq. Gastroenterol."],"published-print":{"date-parts":[[2013,9]]},"abstract":"<jats:p>Context Hepatitis B virus (HBV) can cause fulminant hepatitis, cirrhosis and hepatocellular carcinoma, and is one of the most common causes of acute and chronic liver failure. The genetic variants of HBV can be decisive for the evolution of these diseases as well as for the election of therapy. Objectives The aim of this study was to evaluate and standardize an in house methodology based on the analysis of the melting curve polymerase chain reaction (PCR) of real-time (qPCR) to screen for genotypes A, D and F of HBV in patients from a hospital in Rio Grande do Sul, Brazil. Methods We evaluated 104 patients presumably with HBV chronic infection. Viral DNA was extracted from plasma and viral genotypes and different mutations were determined using PCR-based protocols. Results A PCR-based methodology was standardized for the analysis of genotypes A, D and F of HBV. The technique was based in a nested PCR with the final step consisting of a multiplex real-time PCR, using the melting curve as a tool for the differentiation of fragments. A higher frequency of genotype D (44.4%), followed by genotype A (22.2%) and genotype F (3.7%) was observed. Conclusion The standardized assay, a nested PCR-multiplex qPCR using specific primers, provides a rapid and accurate method for the differentiation of HBV genotypes that are more frequent in Southern Brazil \u2013 A, D and F. This method can be applied in the clinical practice.<\/jats:p>","DOI":"10.1590\/s0004-28032013000200039","type":"journal-article","created":{"date-parts":[[2014,1,14]],"date-time":"2014-01-14T18:42:08Z","timestamp":1389724928000},"page":"219-225","source":"Crossref","is-referenced-by-count":4,"title":["MELTING CURVE ANALYSIS FOR THE SCREENING OF HEPATITIS B VIRUS GENOTYPES A, D AND F IN PATIENTS FROM A GENERAL HOSPITAL IN SOUTHERN BRAZIL"],"prefix":"10.1590","volume":"50","author":[{"given":"Carlos Eduardo","family":"BECKER","sequence":"first","affiliation":[{"name":"Universidade Federal de Ciencias da Saude de Porto Alegre"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nelson Alexandre","family":"KRETZMANN","sequence":"additional","affiliation":[{"name":"Universidade Federal de Ciencias da Saude de Porto Alegre"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angelo Alves de","family":"MATTOS","sequence":"additional","affiliation":[{"name":"Universidade Federal de Ciencias da Saude de Porto Alegre"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana Beatriz Gorini da","family":"VEIGA","sequence":"additional","affiliation":[{"name":"Universidade Federal de Ciencias da Saude de Porto Alegre; Universidade Federal de Ciencias da Saude de Porto Alegre"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1016\/j.annepidem.2009.01.017","article-title":"Rocks along the road to the control of HBV and HCC","volume":"19","author":"Beasley RP","year":"2009","journal-title":"Ann Epidemiol"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1590\/S0004-28032010000100003","article-title":"Genotyping of hepatitis B virus in a cohort of patients evaluated in a hospital of Porto Alegre, South of Brazil","volume":"47","author":"Becker C","year":"2010","journal-title":"Arq Gastroenterol"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1111\/j.1365-2893.1997.tb00155.x","article-title":"The clinical significance of surface antigen variants of hepatitis B virus","volume":"4","author":"Carman WF","year":"1997","journal-title":"J Viral Hepat"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1186\/1471-2458-4-13","article-title":"Hepatitis B virus infection in Haemodialysis Centres from Santa Catarina State, Southern Brazil. 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