{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,28]],"date-time":"2025-09-28T08:32:45Z","timestamp":1759048365141},"reference-count":31,"publisher":"FapUNIFESP (SciELO)","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arq. Neuro-Psiquiatr."],"published-print":{"date-parts":[[2011,6]]},"abstract":"<jats:p>OBJECTIVE: To evaluate the performance of two antigenic preparations (vesicular fluid - VF and a glycoprotein fraction, LLa-Gp fraction, purified from a whole parasite extract by lentil lectin affinity chromatography) from Taenia solium cysticerci for the immunodiagnosis of neurocysticercosis. METHOD: Fifty-six cerebrospinal fluid (CSF) samples (22 from patients with neurocysticercosis and 34 from patients with other neurological disorders) and 57 serum samples (22 from patients with neurocysticercosis, 18 from patients with other infections and 17 from presumably healthy persons) were assayed for anticysticercal IgG antibodies with an enzyme-linked immunosorbent assay (ELISA). RESULTS: The VF ELISA showed 100% sensitivity and specificity in CSF and serum samples, whereas the sensitivity and specificity of the LLa-Gp ELISA were, respectively, 90.9% and 97.1%, with the CSF samples and 95.5% and 100% with serum samples. There was no significant difference in the sensitivity and specificity of the two antigenic preparations used to screen CSF and serum samples. CONCLUSION: Considering the complexity and high cost of obtaining the LLa-Gp fraction, VF could be more suitable for screening specific antibodies by ELISA in CSF and serum samples from patients with neurocysticercosis.<\/jats:p>","DOI":"10.1590\/s0004-282x2011000400012","type":"journal-article","created":{"date-parts":[[2011,7,7]],"date-time":"2011-07-07T16:34:44Z","timestamp":1310056484000},"page":"470-474","source":"Crossref","is-referenced-by-count":9,"title":["Evaluation of two Taenia solium cysticercal antigenic preparations (vesicular fluid and a glycoprotein fraction with affinity for lentil lectin) for the immunodiagnosis of neurocysticercosis by enzyme-linked immunosorbent assay (ELISA)"],"prefix":"10.1590","volume":"69","author":[{"given":"Lisandra Akemi","family":"Suzuki","sequence":"first","affiliation":[{"name":"State University of Campinas, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cl\u00e1udio 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update","volume":"2","author":"Carpio A","year":"2002","journal-title":"Lancet Infect Dis"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"288","DOI":"10.1136\/jnnp.2006.101253","article-title":"Neurocysticercosis: some of the essentials","volume":"6","author":"Garcia HH","year":"2006","journal-title":"Pract Neurol"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"965","DOI":"10.4269\/ajtmh.1986.35.965","article-title":"Reliable serology of Taenia solium cysticercosis with antigens from cyst vesicular fluid: ELISA and hemagglutination tests","volume":"35","author":"Larralde C","year":"1986","journal-title":"Am J Trop Med Hyg","ISSN":"http:\/\/id.crossref.org\/issn\/0002-9637","issn-type":"print"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1093\/infdis\/159.1.50","article-title":"An enzyme-linked immunoelectrotransfer blot assay and glycoprotein antigens for diagnosing human cysticercosis (Taenia solium)","volume":"159","author":"Tsang VC","year":"1989","journal-title":"J Infect Dis","ISSN":"http:\/\/id.crossref.org\/issn\/0022-1899","issn-type":"print"},{"key":"ref7","first-page":"926","article-title":"Immunodiagnosis of human cysticercosis in cerebrospinal fluid","volume":"114","author":"Larralde C","year":"1990","journal-title":"Arch Pathol Lab Med","ISSN":"http:\/\/id.crossref.org\/issn\/0003-9985","issn-type":"print"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"733","DOI":"10.1016\/0020-7519(94)90128-7","article-title":"Reactivity in ELISA and dot blot of purified GP24, an immunodominant antigen of Taenia solium, for the diagnosis of human neurocysticercosis","volume":"24","author":"Plancarte A","year":"1994","journal-title":"Int J Parasitol","ISSN":"http:\/\/id.crossref.org\/issn\/0020-7519","issn-type":"print"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"3324","DOI":"10.1128\/JCM.33.12.3324-3325.1995","article-title":"Antigens from Taenia solium cysticerci used in complement fixation, enzyme-linked immunosorbent assay, and western blot (immunoblot) for diagnosis of neurocysticercosis","volume":"33","author":"Garcia E","year":"1995","journal-title":"J Clin Microbiol","ISSN":"http:\/\/id.crossref.org\/issn\/0095-1137","issn-type":"print"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"291","DOI":"10.4269\/ajtmh.1998.59.291","article-title":"Novel antigens for neurocysticercosis: simple method for preparation and evaluation for serodiagnosis","volume":"59","author":"Ito A","year":"1998","journal-title":"Am J Trop Med Hyg","ISSN":"http:\/\/id.crossref.org\/issn\/0002-9637","issn-type":"print"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1590\/S0004-282X2000000100003","article-title":"Neurocysticercosis: detection of IgG, IgA and IgE antibodies in cerebrospinal fluid, serum and saliva samples by ELISA with Taenia solium and Taenia crassiceps antigens","volume":"58","author":"Bueno EC","year":"2000","journal-title":"Arq Neuropsiquiatr","ISSN":"http:\/\/id.crossref.org\/issn\/0004-282X","issn-type":"print"},{"key":"ref12","first-page":"190","article-title":"Use of Taenia crassiceps cysticercus antigen preparations for detection of antibodies in cerebrospinal fluid samples from patients with neurocysticercosis (Taenia solium)","volume":"9","author":"Pardini AX","year":"2002","journal-title":"Clin Diagn Lab Immunol","ISSN":"http:\/\/id.crossref.org\/issn\/1071-412X","issn-type":"print"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/S0001-706X(02)00092-X","article-title":"Evaluation of an antigen from Taenia crassiceps cysticercus for the serodiagnosis of neurocysticercosis","volume":"83","author":"Peralta RH","year":"2002","journal-title":"Acta Trop","ISSN":"http:\/\/id.crossref.org\/issn\/0001-706X","issn-type":"print"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"276","DOI":"10.4269\/ajtmh.2003.68.276","article-title":"Similar diagnostic performance for neurocysticercosis of three glycoprotein preparations from Taenia solium metacestodes","volume":"68","author":"Villota GE","year":"2003","journal-title":"Am J Trop Med Hyg","ISSN":"http:\/\/id.crossref.org\/issn\/0002-9637","issn-type":"print"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"1005","DOI":"10.1111\/j.1365-3156.2005.01480.x","article-title":"Evaluation of Taenia solium and Taenia crassiceps cysticercal antigens for the serodiagnosis of neurocysticercosis","volume":"10","author":"Arruda GC","year":"2005","journal-title":"Trop Med Int Health","ISSN":"http:\/\/id.crossref.org\/issn\/1360-2276","issn-type":"print"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"3","DOI":"10.4269\/ajtmh.2005.72.3","article-title":"New concepts in the diagnosis and management of neurocysticercosis (Taenia solium)","volume":"72","author":"Garcia HH","year":"2005","journal-title":"Am J Trop Med 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Parasitol"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","article-title":"A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding","volume":"72","author":"Bradford MM","year":"1976","journal-title":"Anal Biochem","ISSN":"http:\/\/id.crossref.org\/issn\/0003-2697","issn-type":"print"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1002\/1097-0142(1950)3:1<32::AID-CNCR2820030106>3.0.CO;2-3","article-title":"Index for rating diagnostic tests","volume":"3","author":"Youden WJ","year":"1950","journal-title":"Cancer","ISSN":"http:\/\/id.crossref.org\/issn\/0008-543X","issn-type":"print"},{"key":"ref24","author":"Fleiss JL","year":"1981"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1212\/WNL.57.2.177","article-title":"Proposed diagnostic criteria for neurocysticercosis","volume":"57","author":"Del Brutto 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