{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T12:01:05Z","timestamp":1772020865462,"version":"3.50.1"},"reference-count":15,"publisher":"FapUNIFESP (SciELO)","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rev. Sa\u00fade P\u00fablica"],"published-print":{"date-parts":[[2002,10]]},"abstract":"<jats:p>OBJETIVO: O microspor\u00eddio Encephalitozoon cuniculi tem sido reconhecido como um pat\u00f3geno oportunista em indiv\u00edduos imunossuprimidos, tais como pacientes com Aids. O objetivo do trabalho foi desenvolver animais farmacologicamente imunossuprimidos como modelo da infec\u00e7\u00e3o natural pelo E. cuniculi. M\u00c9TODOS: Foram usados grupos distintos de camundongos Balb-C adultos, imunossuprimidos com diferentes doses de dexametasona (Dx, 3 ou 5 mg\/kg\/dia por via intraperitoneal &lt;FONT FACE=Symbol&gt;\u00be&lt;\/FONT&gt; IP) e inoculados com esporos de E. cuniculi por via IP. Tamb\u00e9m foram usados grupos controle (animais inoculados, mas n\u00e3oimunossuprimidos, e animais imunossuprimidos, mas n\u00e3o inoculados). Os esporos de E. cuniculi foram previamente cultivados em c\u00e9lulas MDCK. Os animais foram sacrificados e submetidos \u00e0 necropsia aos 7, 14, 21, 28 e 35 dias p\u00f3s-inocula\u00e7\u00e3o. Fragmentos teciduais foram coletados e processados para an\u00e1lise por microscopia de luz, utilizando-se as t\u00e9cnicas de colora\u00e7\u00e3o de Gram -chromotrope e de hematoxilina-eosina. RESULTADOS: Em todos os animais imunossuprimidos e inoculados, por\u00e9m especialmente naqueles que receberam 5 mg\/kg\/dia de Dx, os achados de necropsia mais proeminentes foram hepato e esplenomegalia. A inocula\u00e7\u00e3o experimental resultou em uma infec\u00e7\u00e3o disseminada e n\u00e3o-letal, caracterizada por les\u00f5es granulomatosas em diversos \u00f3rg\u00e3os (f\u00edgado, pulm\u00f5es, rins, intestino, enc\u00e9falo), por\u00e9m mais notadamente no tecido hep\u00e1tico. Esporos de E. cuniculi foram observados em poucos animais tratados com 5 mg\/kg\/dia de Dx aos 35 dias p\u00f3s-infec\u00e7\u00e3o. CONCLUS\u00d5ES: Microsporidiose em camundongos imunossuprimidos com Dx fornece um modelo \u00fatil para estudos da infec\u00e7\u00e3o por microspor\u00eddios, assemelhando-se \u00e0quela naturalmente observada em indiv\u00edduos imunodeficientes com Aids.<\/jats:p>","DOI":"10.1590\/s0034-89102002000600012","type":"journal-article","created":{"date-parts":[[2005,4,27]],"date-time":"2005-04-27T14:24:48Z","timestamp":1114611888000},"page":"621-626","source":"Crossref","is-referenced-by-count":9,"title":["Infec\u00e7\u00e3o experimental pelo Encephalitozoon cuniculi em camundongos imunossuprimidos com dexametasona"],"prefix":"10.1590","volume":"36","author":[{"given":"Maria Anete","family":"Lallo","sequence":"first","affiliation":[{"name":"Universidade Paulista, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maur\u00edcio Jos\u00e9 dos","family":"Santos","sequence":"additional","affiliation":[{"name":"Universidade Paulista, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eduardo Fernandes","family":"Bondan","sequence":"additional","affiliation":[{"name":"Universidade Paulista, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","reference":[{"key":"ref1","series-title":"Celular and molecular immunology","first-page":"341","author":"Abbas AK","year":"1997","edition":"3"},{"key":"ref2","series-title":"Goodman & Gilman's: the pharmacological basis of therapeutics","first-page":"1292","volume-title":"Immunomodulators: immunosuppressive agents and immunostimulants","author":"Diasio RB","year":"1996"},{"key":"ref3","first-page":"1","article-title":"Experimental microsporidiosis in immunocompetent and immunodeficient mice and monkeys","volume":"41","author":"Didier ES","year":"1994","journal-title":"Folia Parasitol"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"709","DOI":"10.1016\/S1286-4579(00)00354-3","article-title":"Microsporidiosis in 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