{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,2]],"date-time":"2023-10-02T01:15:42Z","timestamp":1696209342781},"reference-count":16,"publisher":"FapUNIFESP (SciELO)","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rev. Sa\u00fade P\u00fablica"],"published-print":{"date-parts":[[2009,12]]},"abstract":"<jats:p>OBJETIVO: Analisar a acur\u00e1cia do diagn\u00f3stico de dois protocolos de imunofluoresc\u00eancia indireta para leishmaniose visceral canina. M\u00c9TODOS: C\u00e3es provenientes de inqu\u00e9rito soroepidemiol\u00f3gico realizado em \u00e1rea end\u00eamica nos munic\u00edpios de Ara\u00e7atuba e de Andradina, na regi\u00e3o noroeste do estado de S\u00e3o Paulo, em 2003, e \u00e1rea n\u00e3o end\u00eamica da regi\u00e3o metropolitana de S\u00e3o Paulo, foram utilizados para avaliar comparativamente dois protocolos da rea\u00e7\u00e3o de imunofluoresc\u00eancia indireta (RIFI) para leishmaniose: um utilizando ant\u00edgeno heter\u00f3logo Leishmania major (RIFI-BM) e outro utilizando ant\u00edgeno hom\u00f3logo Leishmania chagasi (RIFI-CH). Para estimar acur\u00e1cia utilizou-se a an\u00e1lise two-graph receiver operating characteristic (TG-ROC). A an\u00e1lise TG-ROC comparou as leituras da dilui\u00e7\u00e3o 1:20 do ant\u00edgeno hom\u00f3logo (RIFI-CH), consideradas como teste refer\u00eancia, com as dilui\u00e7\u00f5es da RIFI-BM (ant\u00edgeno heter\u00f3logo). RESULTADOS: A dilui\u00e7\u00e3o 1:20 do teste RIFI-CH apresentou o melhor coeficiente de conting\u00eancia (0,755) e a maior for\u00e7a de associa\u00e7\u00e3o entre as duas vari\u00e1veis estudadas (qui-quadrado=124,3), sendo considerada a dilui\u00e7\u00e3o-refer\u00eancia do teste nas compara\u00e7\u00f5es com as diferentes dilui\u00e7\u00f5es do teste RIFI-BM. Os melhores resultados do RIFI-BM foram obtidos na dilui\u00e7\u00e3o 1:40, com melhor coeficiente de conting\u00eancia (0,680) e maior for\u00e7a de associa\u00e7\u00e3o (qui-quadrado=80,8). Com a mudan\u00e7a do ponto de corte sugerido nesta an\u00e1lise para a dilui\u00e7\u00e3o 1:40 da RIFI-BM, o valor do par\u00e2metro especificidade aumentou de 57,5% para 97,7%, embora a dilui\u00e7\u00e3o 1:80 tivesse apresentado a melhor estimativa para sensibilidade (80,2%) com o novo ponto de corte. CONCLUS\u00d5ES: A an\u00e1lise TG-ROC pode fornecer importantes informa\u00e7\u00f5es sobre os testes de diagn\u00f3sticos, al\u00e9m de apresentar sugest\u00f5es sobre pontos de cortes que podem melhorar as estimativas de sensibilidade e especificidade do teste, e avali\u00e1-los a luz do melhor custo-benef\u00edcio.<\/jats:p>","DOI":"10.1590\/s0034-89102009005000063","type":"journal-article","created":{"date-parts":[[2009,10,22]],"date-time":"2009-10-22T07:35:52Z","timestamp":1256196952000},"page":"1044-1053","source":"Crossref","is-referenced-by-count":3,"title":["An\u00e1lise TG-ROC de testes de imunofluoresc\u00eancia no diagn\u00f3stico de leishmaniose visceral canina"],"prefix":"10.1590","volume":"43","author":[{"given":"Rita Maria da","family":"Silva","sequence":"first","affiliation":[{"name":"Instituto Adolfo Lutz,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M\u00e1rcia Dalastra","family":"Laurenti","sequence":"additional","affiliation":[{"name":"Universidade S\u00e3o Paulo,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alm\u00e9rio de Castro","family":"Gomes","sequence":"additional","affiliation":[{"name":"USP,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yeda Lopes","family":"Nogueira","sequence":"additional","affiliation":[{"name":"Instituto Adolfo Lutz,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","reference":[{"issue":"1","key":"ref1","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1590\/S0102-311X2004000100043","article-title":"Reflex\u00f5es sobre a qualidade do diagn\u00f3stico da leishmaniose visceral canina em inqu\u00e9ritos epidemiol\u00f3gicos: o caso da epidemia de Belo Horizonte, Minas Gerais, Brasil, 1993-1997","volume":"20","author":"Alves WA","year":"2004","journal-title":"Cad Saude 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