{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T18:21:23Z","timestamp":1793125283461,"version":"4.5.0"},"reference-count":13,"publisher":"FapUNIFESP (SciELO)","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rev. Inst. Med. trop. S. Paulo"],"published-print":{"date-parts":[[1997,9]]},"abstract":"<jats:p>Visceral larva migrans (VLM) is a clinical syndrome caused by infection of man by Toxocara spp, the common roundworm of dogs and cats. Tissue migration of larval stages causes illness specially in children. Because larvae are difficult to detect in tissues, diagnosis is mostly based on serology. After the introduction of the enzyme-linked immunosorbent assay (ELISA) using the larval excretory-secretory antigen of T. canis (TES), the diagnosis specificity was greatly improved although cross-reactivity with other helminths are still being reported. In Brazil, diagnosis is routinely made after absorption of serum samples with Ascaris suum antigens, a nematode antigenicaly related with Ascaris lumbricoides which is a common intestinal nematode of children. In order to identify T. canis antigens that cross react to A. suum antigens we analyzed TES antigen by SDS-PAGE and Western blotting techniques. When we used serum samples from patients suspected of VLM and positive result by ELISA as well as a reference serum sample numerous bands were seen (molecular weight of 210-200 kDa, 116-97 kDa, 55-50 kDa and 35-29 kDa). Among these there is at least one band with molecular weight around 55-66 kDa that seem to be responsible for the cross-reactivity between T. canis e A. suum once it disappears when previous absorption of serum samples with A. suum antigens is performed<\/jats:p>","DOI":"10.1590\/s0036-46651997000500002","type":"journal-article","created":{"date-parts":[[2005,5,9]],"date-time":"2005-05-09T14:14:05Z","timestamp":1115648045000},"page":"253-256","source":"Crossref","is-referenced-by-count":36,"title":["Cross-Reactions between Toxocara canis and Ascaris suum in the diagnosis of visceral larva migrans by western blotting technique"],"prefix":"10.1590","volume":"39","author":[{"given":"C\u00e1ris Maroni","family":"NUNES","sequence":"first","affiliation":[{"name":"S\u00e3o Paulo State University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Regina Nardini","family":"TUNDISI","sequence":"additional","affiliation":[{"name":"Instituto Adolfo Lutz de S\u00e3o Paulo"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Fernando","family":"GARCIA","sequence":"additional","affiliation":[{"name":"S\u00e3o Paulo State University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcos Brayan","family":"HEINEMANN","sequence":"additional","affiliation":[{"name":"University of S\u00e3o Paulo"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saemi","family":"OGASSAWARA","sequence":"additional","affiliation":[{"name":"University of S\u00e3o Paulo"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leonardo Jos\u00e9","family":"RICHTZENHAIN","sequence":"additional","affiliation":[{"name":"University of S\u00e3o Paulo"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"530","reference":[{"key":"ref1","series-title":"Desenvolvimento de teste imunoenzim\u00e1tico, ELISA, para o diagn\u00f3stico da toxocar\u00edase humana","author":"BACH-RIZZATTI B. 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Immunol."},{"key":"ref8","doi-asserted-by":"crossref","first-page":"697","DOI":"10.1007\/BF00928685","article-title":"Application of the western blotting procedure for the immunodiagnosis of human toxocariasis","volume":"77","author":"MAGNAVAL J. F.","year":"1991","journal-title":"Parasit. Res."},{"key":"ref9","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1111\/j.1365-3024.1984.tb00779.x","article-title":"Characterization of surface and excretory-secretory antigens of Toxocara canis infective larvae","volume":"6","author":"MAIZELS R. M.","year":"1984","journal-title":"Paras. Immunol."},{"key":"ref10","doi-asserted-by":"crossref","first-page":"781","DOI":"10.2307\/3279492","article-title":"In vitro maintenance of Toxocara canis larvae and a simple method for the production of Toxocara ES antigens for use in serodiagnostic tests for visceral larva migrans","volume":"61","author":"SAVIGNY D. H. de","year":"1975","journal-title":"J. Parasit."},{"key":"ref11","doi-asserted-by":"crossref","first-page":"21","DOI":"10.4269\/ajtmh.1989.41.21","article-title":"Toxocara larva migrans now","volume":"4","author":"SCHANTZ P. M.","year":"1989","journal-title":"Amer. J. trop. Med. Hyg.","ISSN":"https:\/\/id.crossref.org\/issn\/0002-9637","issn-type":"print"},{"key":"ref12","first-page":"361","article-title":"A collaborative study on larval excretory\/secretory antigens of Toxocara canis for the immunodiagnosis of human toxocariasis with ELISA","volume":"41","author":"SPEISER F.","year":"1984","journal-title":"Acta trop."},{"key":"ref13","doi-asserted-by":"crossref","first-page":"4350","DOI":"10.1073\/pnas.76.9.4350","article-title":"Electrophoretic transfer of proteins from polyacrilamyde gels to nitrocellulose sheets: procedure and some applications","volume":"76","author":"TOWBIN H.","year":"1979","journal-title":"Proc. nat. Acad. Sci (Wash.)"}],"container-title":["Revista do Instituto de Medicina Tropical de S\u00e3o Paulo"],"original-title":[],"link":[{"URL":"http:\/\/www.scielo.br\/pdf\/rimtsp\/v39n5\/MT-02.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2017,5,24]],"date-time":"2017-05-24T14:28:56Z","timestamp":1495636136000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_arttext&pid=S0036-46651997000500002&lng=en&tlng=en"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,9]]},"references-count":13,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1997,9]]}},"alternative-id":["S0036-46651997000500002"],"URL":"https:\/\/doi.org\/10.1590\/s0036-46651997000500002","relation":{},"ISSN":["0036-4665"],"issn-type":[{"value":"0036-4665","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,9]]}}}