{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T05:33:34Z","timestamp":1768455214653,"version":"3.49.0"},"reference-count":30,"publisher":"FapUNIFESP (SciELO)","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rev. Inst. Med. trop. S. Paulo"],"published-print":{"date-parts":[[2004,8]]},"abstract":"<jats:p>Cryptococcus neoformans detection was optimized using PCR technique with the objective of application in the clinical laboratory diagnosis. The amplification area was ITS and 5,6S which encodes the ribosomal RNA (rRNA). A total of 72 cerebrospinal fluid (CSF) samples were used, obtained from cases with and without AIDS. The patients had cryptococcal meningitis (n = 56) and meningitis caused by other agents (n = 16). The results demonstrated that PCR test had the highest sensitivity rates, superior to culture (85.7%) and to India ink test (76.8%). PCR was found to be sensitive in detecting 1 cell\/mL and highly specific since it did not amplify other fungal DNA. The comparative analysis of the methods showed that PCR is more sensitive and specific and is applicable as an important laboratorial resource for neurocryptococcosis diagnosis.<\/jats:p>","DOI":"10.1590\/s0036-46652004000400006","type":"journal-article","created":{"date-parts":[[2005,4,27]],"date-time":"2005-04-27T13:34:01Z","timestamp":1114608841000},"page":"203-207","source":"Crossref","is-referenced-by-count":21,"title":["Neurocryptococcosis: diagnosis by PCR method"],"prefix":"10.1590","volume":"46","author":[{"given":"Regina C\u00e9lia","family":"Paschoal","sequence":"first","affiliation":[{"name":"University of S\u00e3o Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M\u00e1rio Hiroyuki","family":"Hirata","sequence":"additional","affiliation":[{"name":"University of S\u00e3o Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ros\u00e1rio Crespo","family":"Hirata","sequence":"additional","affiliation":[{"name":"University of S\u00e3o Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M\u00e1rcia de Souza Carvalho","family":"Melhem","sequence":"additional","affiliation":[{"name":"Adolfo Lutz Institute, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amanda Latercia Tranches","family":"Dias","sequence":"additional","affiliation":[{"name":"USP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudete Rodrigues","family":"Paula","sequence":"additional","affiliation":[{"name":"USP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","reference":[{"key":"ref1","series-title":"- Ocorr\u00eancia de Cryptococcus neoformans em excretas de pombos localizados em torres de igrejas na cidade do Rio de Janeiro: fatores de virul\u00eancia e sensibilidade aos antif\u00fangicos","author":"BARONI F.A.","year":"2001"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"1356","DOI":"10.1128\/JCM.42.3.1356-1359.2004","article-title":"Cryptococcus neoformans shows a remarkable genotypic diversity in Brazil","volume":"42","author":"BARRETO DE OLIVEIRA M.T.","year":"2004","journal-title":"J. clin. 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