{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T22:24:03Z","timestamp":1791239043925,"version":"4.1.0"},"reference-count":25,"publisher":"FapUNIFESP (SciELO)","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rev. Inst. Med. trop. S. Paulo"],"published-print":{"date-parts":[[2007,8]]},"abstract":"<jats:p>Cryptococcus neoformans is an encapsulated yeast, etiological agent of cryptococcosis. The species is commonly associated with pigeon droppings and plant materials. The aim of the present work was to verify the presence of the yeast in pigeon droppings, and to identify the isolates obtained in serotypes and mating types (MAT). Ten samples of pigeon droppings were collected in the rural area of the city of Alfenas, Brazil. Samples were inoculated in agar Niger medium for fungal isolation and 22 isolates with characteristics of C. neoformans were obtained. The serotypes and MAT were determined by multiplex PCR using specific primers. Serotypes were also determined by using the Kit Crypto Check. Among the 22 samples evaluated, eight were identified as C. neoformans by classic identification tests. These samples were characterized as serotype A by the Kit Crypto check and as serotype A MAT alpha by the multiplex PCR. The present study reinforces the evidence that pigeon droppings are a reservoir for C. neoformans and confirms the prevalence of C. neoformans var. grubii (Aalpha) among environmental isolates. It also demonstrates that multiplex PCR is an acceptable alternative for serotype analysis because it reduces the costs for each reaction and analyses serotype and MAT simultaneously.<\/jats:p>","DOI":"10.1590\/s0036-46652007000400002","type":"journal-article","created":{"date-parts":[[2007,10,4]],"date-time":"2007-10-04T16:34:39Z","timestamp":1191515679000},"page":"207-210","source":"Crossref","is-referenced-by-count":15,"title":["Serotype and mating type characterization of Cryptococcus neoformans by Multiplex PCR"],"prefix":"10.1590","volume":"49","author":[{"given":"V\u00edvian Gon\u00e7alves","family":"Carvalho","sequence":"first","affiliation":[{"name":"Universidade Federal de Alfenas, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mateus Souza","family":"Terceti","sequence":"additional","affiliation":[{"name":"Universidade Federal de Alfenas, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amanda Latercia Tranches","family":"Dias","sequence":"additional","affiliation":[{"name":"Universidade Federal de Alfenas, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudete Rodrigues","family":"Paula","sequence":"additional","affiliation":[{"name":"Universidade de S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juliana Pereira","family":"Lyon","sequence":"additional","affiliation":[{"name":"Universidade Federal de Alfenas, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ant\u00f4nio Martins de","family":"Siqueira","sequence":"additional","affiliation":[{"name":"Universidade Federal de Alfenas, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00edlia Caixeta","family":"Franco","sequence":"additional","affiliation":[{"name":"Universidade Federal de Alfenas, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"530","reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"1356","DOI":"10.1128\/JCM.42.3.1356-1359.2004","article-title":"Cryptococcus neoformans shows a remarkable genotypic diversity in Brazil","volume":"42","author":"BARRETO DE OLIVEIRA M.T.","year":"2004","journal-title":"J. clin. Microbiol.","ISSN":"https:\/\/id.crossref.org\/issn\/0095-1137","issn-type":"print"},{"key":"ref2","first-page":"221","article-title":"Rapid methods to extract DNA and RNA from Cryptococcus neoformans","volume":"1","author":"BOLANO A.","year":"2001","journal-title":"FEMS Microbiol. Lett.","ISSN":"https:\/\/id.crossref.org\/issn\/0378-1097","issn-type":"print"},{"key":"ref3","first-page":"227","article-title":"Saprophytic sources of Cryptococcus neoformans associated with the pigeon (Columba livia)","volume":"62","author":"EMMONS C.W.","year":"1955","journal-title":"Amer. J. Hyg.","ISSN":"https:\/\/id.crossref.org\/issn\/0096-5294","issn-type":"print"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1590\/S0037-86822002000600008","article-title":"Cativeiros de aves como fonte de Cryptococcus neoformans na cidade de Campo Grande, Mato Grosso do Sul, Brasil","volume":"35","author":"FILI\u00da W.F.O.","year":"2002","journal-title":"Rev. Soc. bras. Med. trop.","ISSN":"https:\/\/id.crossref.org\/issn\/0037-8682","issn-type":"print"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"838","DOI":"10.1128\/JCM.37.3.838-840.1999","article-title":"Cryptococcus neoformans var. grubii: separate varietal status for Cryptococcus neoformans serotype A isolates","volume":"37","author":"FRANZOT S.P","year":"1999","journal-title":"J. clin. Microbiol.","ISSN":"https:\/\/id.crossref.org\/issn\/0095-1137","issn-type":"print"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1023\/A:1006863705759","article-title":"Isolation of Cryptococcus neoformans from pigeon (Columba livia) droppings in northern Iran","volume":"139","author":"KHOSRAVI A.R.","year":"1997","journal-title":"Mycopathologia (Den Haag)"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1590\/S0036-46652005000400005","article-title":"Characterization of Cryptococcus neoformans isolated from urban environmental sources in Goi\u00e2nia, Goi\u00e1s State, Brazil","volume":"47","author":"KOBAYASHI C.C.B.A.","year":"2005","journal-title":"Rev. Inst. Med. trop. S. Paulo","ISSN":"https:\/\/id.crossref.org\/issn\/0036-4665","issn-type":"print"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1093\/oxfordjournals.aje.a112628","article-title":"Distribution of alpha and alpha mating types of Cryptococcus neoformans among natural and clinical isolates","volume":"108","author":"KWON-CHUNG K.J.","year":"1978","journal-title":"Amer. J. Epidem."},{"key":"ref9","doi-asserted-by":"crossref","first-page":"574","DOI":"10.1111\/j.1567-1364.2006.00088.x","article-title":"Do major species concepts support one, two or more species within Cryptococcus neoformans?","volume":"6","author":"KWON-CHUNG K.J.","year":"2006","journal-title":"FEMS Yeast Res."},{"key":"ref10","series-title":"Micologia m\u00e9dica","author":"LACAZ C.S.","year":"1991"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1080\/mmy.38.5.385.390","article-title":"Environmental isolation of Cryptococcus neoformans var. gattii and C. neoformans var. neoformans in the city of S\u00e3o Paulo, Brazil","volume":"38","author":"MONTENEGRO H.","year":"2000","journal-title":"Med. Mycol."},{"key":"ref12","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1128\/JCM.41.1.73-77.2003","article-title":"Serotyping of 467 Cryptococcus neoformans isolates from clinical and environmental sources in Brazil: analysis of host and regional patterns","volume":"41","author":"NISHIKAWA M.M.","year":"2003","journal-title":"J. clin. Microbiol.","ISSN":"https:\/\/id.crossref.org\/issn\/0095-1137","issn-type":"print"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1590\/S0036-46652002000600001","article-title":"Serotype, mating type and ploidy of Cryptococcus neoformans strains isolated from patients in Brazil","volume":"44","author":"OHKUSU M.","year":"2002","journal-title":"Rev. Inst. Med. trop. S. Paulo","ISSN":"https:\/\/id.crossref.org\/issn\/0036-4665","issn-type":"print"},{"key":"ref14","first-page":"181","article-title":"First report of isolation of Cryptococcus neoformans var. neoformans from avian excreta in Kathmandu, Nepal","volume":"14","author":"PAL M.","year":"1997","journal-title":"Rev. iberoamer. Micol."},{"key":"ref15","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1080\/369378031000137251","article-title":"Decayed wood of Syzygium cumini and Ficus religiosa living trees in Delhi\/New Delhi metropolitan area as natural habitat of Cryptococcus neoformans","volume":"41","author":"RANDHAWA H.S.","year":"2003","journal-title":"Med. Mycol."},{"key":"ref16","series-title":"Isolamento de Cryptococcus neoformans de fezes de pombos e can\u00e1rios do munic\u00edpio de Alfenas-MG","author":"REZENDE D.G.","year":"2002"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1093\/clinids\/18.3.369","article-title":"Clinical epidemiological study of 171 cases of cryptococcosis","volume":"18","author":"ROZENBAUM R.","year":"1994","journal-title":"Clin. infect. Dis.","ISSN":"https:\/\/id.crossref.org\/issn\/1058-4838","issn-type":"print"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1126\/science.151.3707.208","article-title":"Medium for selective isolation of Cryptococcus neoformans","volume":"151","author":"SHIELDS A.B.","year":"1966","journal-title":"Science","ISSN":"https:\/\/id.crossref.org\/issn\/0036-8075","issn-type":"print"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1590\/S0036-46652005000100006","article-title":"Environmental strains of Cryptococcus neoformans var. grubii in the city of Santos, SP, Brazil","volume":"47","author":"SOARES M.C.B.","year":"2005","journal-title":"Rev. Inst. Med. trop. S. 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Bact.","ISSN":"https:\/\/id.crossref.org\/issn\/0021-9193","issn-type":"print"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"965","DOI":"10.1128\/JCM.40.3.965-972.2002","article-title":"Geographic distribution of mating type alleles of Cryptococcus neoformans in four areas of the United States","volume":"40","author":"YAN Z.","year":"2002","journal-title":"J. clin. Microbiol.","ISSN":"https:\/\/id.crossref.org\/issn\/0095-1137","issn-type":"print"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1080\/02681219880000621","article-title":"Isolation of Cryptococcus neoformans var. neoformans from pigeon droppings collected throughout Turkey","volume":"36","author":"YILDIRAN S.T.","year":"1998","journal-title":"Med. 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