{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T20:02:05Z","timestamp":1787860925818,"version":"build-2784847793"},"reference-count":31,"publisher":"FapUNIFESP (SciELO)","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rev. Inst. Med. trop. S. Paulo"],"published-print":{"date-parts":[[2008,4]]},"abstract":"<jats:p>Rocio virus (ROCV) was responsible for an explosive encephalitis epidemic in the 1970s affecting about 1,000 residents of 20 coastland counties in S\u00e3o Paulo State, Brazil. ROCV was first isolated in 1975 from the cerebellum of a fatal human case of encephalitis. Clinical manifestations of the illness are similar to those described for St. Louis encephalitis. ROCV shows intense antigenic cross-reactivity with Japanese encephalitis complex (JEC) viruses, particularly with Ilheus (ILHV), St. Louis encephalitis, Murray Valley and West Nile viruses. In this study, we report a specific RT-PCR assay for ROCV diagnosis and the molecular characterization of the SPAn37630 and SPH37623 strains. Partial nucleotide sequences of NS5 and E genes determined from both strains were used in phylogenetic analysis. The results indicated that these strains are closely related to JEC viruses, but forming a distinct subclade together with ILHV, in accordance with results recently reported by Medeiros et al. (2007).<\/jats:p>","DOI":"10.1590\/s0036-46652008000200005","type":"journal-article","created":{"date-parts":[[2008,5,15]],"date-time":"2008-05-15T03:27:06Z","timestamp":1210822026000},"page":"89-94","source":"Crossref","is-referenced-by-count":7,"title":["Molecular characterization of two rocio flavivirus strains isolated during the encephalitis epidemic in s\u00e3o paulo state, brazil and the development of a one-step rt-pcr assay for diagnosis"],"prefix":"10.1590","volume":"50","author":[{"given":"Terezinha Lisieux Moraes","family":"Coimbra","sequence":"first","affiliation":[{"name":"Instituto Adolfo Lutz, Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raimundo N.","family":"Santos","sequence":"additional","affiliation":[{"name":"Instituto Adolfo Lutz, Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Selma","family":"Petrella","sequence":"additional","affiliation":[{"name":"Instituto Adolfo Lutz, Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teresa Keico","family":"Nagasse-Sugahara","sequence":"additional","affiliation":[{"name":"Instituto Adolfo Lutz, Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvana Beres","family":"Castrignano","sequence":"additional","affiliation":[{"name":"Instituto Adolfo Lutz, Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cec\u00edlia L. Sim\u00f5es","family":"Santos","sequence":"additional","affiliation":[{"name":"Instituto Adolfo Lutz, Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"530","reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1590\/S0074-02762003000300015","article-title":"Brazilian flavivirus phylogeny based on NS5","volume":"98","author":"BALEOTTI F.G.","year":"2003","journal-title":"Mem. Inst. Oswaldo Cruz","ISSN":"https:\/\/id.crossref.org\/issn\/0074-0276","issn-type":"print"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/S0168-1702(01)00222-2","article-title":"Phylogenetic analysis of Brazilian flavivirus using nucleotide sequences of parts of NS5 gene and 3' non-coding regions","volume":"75","author":"BATISTA W.C.","year":"2001","journal-title":"Virus Res."},{"key":"ref3","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1099\/0022-1317-70-1-37","article-title":"Antigenic relationships between flaviviruses as determined by cross-neutralization tests with polyclonal antisera","volume":"70","author":"CALISHER C.H.","year":"1989","journal-title":"J. gen. Virol."},{"key":"ref4","series-title":"An overview of arbovirology in Brazil and neighbouring countries","first-page":"168","volume-title":"Arthropod-and-rodent-borne viruses detected in S\u00e3o Paulo State, Brazil","author":"COIMBRA T.L.M.","year":"1988"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"696","DOI":"10.1128\/JCM.43.2.696-702.2005","article-title":"Duplex reverse transcription-PCR followed by nested PCR assays for detection and identification of Brazilian alphaviruses and flaviviruses","volume":"43","author":"DE MORAIS BRONZONI R.V.","year":"2005","journal-title":"J. clin. Microbiol.","ISSN":"https:\/\/id.crossref.org\/issn\/0095-1137","issn-type":"print"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"394","DOI":"10.1093\/oxfordjournals.aje.a112637","article-title":"Emergence of a new arbovirus disease in Brazil: II. Epidemiologic studies on 1975 epidemic","volume":"108","author":"DE SOUZA LOPES O.","year":"1978","journal-title":"Amer. J. Epidem."},{"key":"ref7","doi-asserted-by":"crossref","first-page":"444","DOI":"10.1093\/oxfordjournals.aje.a112563","article-title":"Emergence of a new arbovirus disease in Brazil: I. Isolation and characterization of the etiologic agent, Rocio virus","volume":"107","author":"DE SOUZA LOPES O.","year":"1978","journal-title":"Amer. J. Epidem."},{"key":"ref8","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1093\/oxfordjournals.aje.a113075","article-title":"Emergence of a new arbovirus disease in Brazil: III. Isolation of Rocio from Psorophora ferox (Humboldt, 1819)","volume":"113","author":"DE SOUZA LOPES O.","year":"1981","journal-title":"Amer. J. Epidem."},{"key":"ref9","doi-asserted-by":"crossref","first-page":"2129","DOI":"10.1126\/science.287.5461.2129a","article-title":"New York's deadly virus may stage a comeback","volume":"287","author":"ENSERINK M.","year":"2000","journal-title":"Science","ISSN":"https:\/\/id.crossref.org\/issn\/0036-8075","issn-type":"print"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"783","DOI":"10.2307\/2408678","article-title":"Confidence limits on phylogenies: an approach using the bootstrap","volume":"39","author":"FELSENSTEIN J.","year":"1985","journal-title":"Evolution","ISSN":"https:\/\/id.crossref.org\/issn\/0014-3820","issn-type":"print"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"406","DOI":"10.2307\/2412116","article-title":"Toward defining the course of evolution: minimum change for a specific tree topology","volume":"20","author":"FITCH W.M.","year":"1971","journal-title":"Syst. Zool.","ISSN":"https:\/\/id.crossref.org\/issn\/0039-7989","issn-type":"print"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1590\/S0034-89101978000300005","article-title":"Estudos ecol\u00f3gicos sobre mosquitos Culicidae no sistema da Serra do Mar, Brasil","volume":"12","author":"FORATTINI O.P.","year":"1978","journal-title":"Rev. Sa\u00fade p\u00fabl. (S. Paulo)","ISSN":"https:\/\/id.crossref.org\/issn\/0034-8910","issn-type":"print"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"1867","DOI":"10.1099\/0022-1317-82-8-1867","article-title":"Phylogenetic relationships of flaviviruses correlate with their epidemiology, disease association and biogeography","volume":"82","author":"GAUNT M.W.","year":"2001","journal-title":"J. gen. Virol."},{"key":"ref14","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1016\/j.jviromet.2005.04.006","article-title":"Rapid subgroup identification of the flaviviruses using degenerate primer E-gene RT-PCR and site specific restriction enzyme analysis","volume":"128","author":"GAUNT M.W.","year":"2005","journal-title":"J. virol. Meth.","ISSN":"https:\/\/id.crossref.org\/issn\/0166-0934","issn-type":"print"},{"key":"ref15","first-page":"95","article-title":"BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95\/98\/NT","volume":"41","author":"HALL T.A.","year":"1999","journal-title":"Nucleic Acids Symp. Ser."},{"key":"ref16","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1590\/S0034-89101980000100002","article-title":"Aspectos da epidemia de encefalite por arbov\u00edrus na regi\u00e3o do vale do Ribeira, S. Paulo, Brasil, no per\u00edodo de 1975 a 1978","volume":"14","author":"IVERSSON L.B.","year":"1980","journal-title":"Rev. Sa\u00fade p\u00fabl. (S. Paulo)","ISSN":"https:\/\/id.crossref.org\/issn\/0034-8910","issn-type":"print"},{"key":"ref17","series-title":"The arboviruses: epidemiology and ecology","first-page":"77","volume-title":"Rocio encephalitis","volume":"4","author":"IVERSSON L.B.","year":"1988"},{"key":"ref18","series-title":"Veronesi: tratado de infectologia","first-page":"373","volume-title":"Encefalite por arbov\u00edrus Rocio","volume":"1","author":"IVERSSON L.B.","year":"2005","edition":"3"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1128\/JVI.72.1.73-83.1998","article-title":"Phylogeny of the genus Flavivirus","volume":"72","author":"KUNO G.","year":"1998","journal-title":"J. Virol."},{"key":"ref20","doi-asserted-by":"crossref","first-page":"2237","DOI":"10.1099\/vir.0.82883-0","article-title":"Complete genome characterization of Rocio virus (Flavivirus: Flaviviridae), a Brazilian flavivirus isolated from a fatal case of encephalitis during an epidemic in S\u00e3o Paulo State","volume":"88","author":"MEDEIROS D.B.A.","year":"2007","journal-title":"J. gen. Virol."},{"key":"ref21","doi-asserted-by":"crossref","first-page":"465","DOI":"10.4269\/ajtmh.1981.30.465","article-title":"Experimental transmission of Rocio virus by mosquitoes","volume":"30","author":"MITCHELL C.J.","year":"1981","journal-title":"Amer. J. trop. Med. Hyg.","ISSN":"https:\/\/id.crossref.org\/issn\/0002-9637","issn-type":"print"},{"key":"ref22","series-title":"Field's Virology","first-page":"1043","volume-title":"Flaviviruses","volume":"2","author":"MONATH T.P.","year":"2001","edition":"4"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1089\/vbz.2007.0206","article-title":"A real-time RT-PCR method for the universal detection and identification of flaviviruses","volume":"7","author":"MOUREAU G.","year":"2007","journal-title":"Vector Borne Zoonotic Dis."},{"key":"ref24","series-title":"Molecular cloning: a laboratory manual","first-page":"5.10","author":"SAMBROOK J.","year":"2001","edition":"3"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"511","DOI":"10.1590\/S0037-86821997000600012","article-title":"Evid\u00eancias sorol\u00f3gicas da circula\u00e7\u00e3o do arbov\u00edrus Rocio (Flaviviridae) na Bahia","volume":"30","author":"STRAATMANN A.","year":"1997","journal-title":"Rev. Soc. bras. Med. trop.","ISSN":"https:\/\/id.crossref.org\/issn\/0037-8682","issn-type":"print"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"2127","DOI":"10.3201\/eid1012.040295","article-title":"Identifying rodent hantavirus reservoirs, Brazil","volume":"10","author":"SUZUKI A.","year":"2004","journal-title":"Emerg. infect. Dis."},{"key":"ref27","series-title":"PAUP*: phylogenetic analysis using parsimony (and other methods)","author":"SWOFFORD D.L.","year":"2003"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"4876","DOI":"10.1093\/nar\/25.24.4876","article-title":"The Clustal X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools","volume":"25","author":"THOMPSON J.D.","year":"1997","journal-title":"Nucleic Acids Res.","ISSN":"https:\/\/id.crossref.org\/issn\/0305-1048","issn-type":"print"},{"key":"ref29","first-page":"415","article-title":"Encefalite humana prim\u00e1ria epid\u00eamica observada no litoral sul do Estado de S\u00e3o Paulo","volume":"22","author":"TIRIBA A.C.","year":"1976","journal-title":"Rev. Ass. m\u00e9d. bras."},{"key":"ref30","first-page":"117","article-title":"Clinical and ecoepidemiological situation of human arboviruses in Brazilian Amazonia","volume":"44","author":"VASCONCELOS P.F.C.","year":"1992","journal-title":"Ci\u00eanc. e Cult."},{"key":"ref31","doi-asserted-by":"crossref","first-page":"548","DOI":"10.1073\/pnas.93.2.548","article-title":"Population dynamics of flavivirus revealed by molecular phylogenies","volume":"93","author":"ZANOTTO P.M.A.","year":"1996","journal-title":"Proc. nat. Acad. Sci. (Wash.)","ISSN":"https:\/\/id.crossref.org\/issn\/0027-8424","issn-type":"print"}],"container-title":["Revista do Instituto de Medicina Tropical de S\u00e3o Paulo"],"original-title":[],"link":[{"URL":"http:\/\/www.scielo.br\/pdf\/rimtsp\/v50n2\/a05v50n2.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,5,5]],"date-time":"2020-05-05T17:56:53Z","timestamp":1588701413000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_arttext&pid=S0036-46652008000200005&lng=en&tlng=en"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,4]]},"references-count":31,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2008,4]]}},"alternative-id":["S0036-46652008000200005"],"URL":"https:\/\/doi.org\/10.1590\/s0036-46652008000200005","relation":{},"ISSN":["0036-4665"],"issn-type":[{"value":"0036-4665","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,4]]}}}