{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T12:01:32Z","timestamp":1762430492226},"reference-count":34,"publisher":"FapUNIFESP (SciELO)","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rev. Inst. Med. trop. S. Paulo"],"published-print":{"date-parts":[[2010,10]]},"abstract":"<jats:p>Canine visceral leishmaniasis (CVL) is recognizable by characteristic signs of disease and is highly lethal. The infection, however, may be quite inapparent in some seropositive dogs, and this has raised the polemic question as to whether or not such animals can be a source of infection for Lutzomyia longipalpis, the vector of American visceral leishmaniasis (AVL). In this study we have examined 51 dogs with acute CVL from an AVL area in Par\u00e1 State, northern Brazil, and compared the parasite density, amastigotes of Leishmania (L.) infantum chagasi, in the skin, lymph node and viscera of symptomatic with that of nine asymptomatic but seropositive dogs (IFAT-IgG). Post-mortem biopsy fragments of these tissues were processed by immunohistochemistry, using a polyclonal antibody against Leishmania sp. The X\u00b2 and Mann Whitney tests were used to evaluate the means of infected macrophage density (p &lt; 0.05). There was no difference (p &gt; 0.05) in the skin (10.7\/mm\u00b2 x 15.5\/mm\u00b2) and lymph node (6.3\/mm\u00b2 x 8.3\/mm\u00b2), between asymptomatic and symptomatic dogs, respectively. It was higher (p &lt; 0.05), however, in the viscera of symptomatic (5.3\/mm\u00b2) than it was in asymptomatic (1.4\/mm\u00b2) dogs. These results strongly suggest that asymptomatic or symptomatic L. (L.) i. chagasi-infected dogs can serve as a source of infection, principally considering the highest (p &lt; 0.05) parasite density from skin (10.7\/mm\u00b2 x 15.5\/mm\u00b2), the place where the vetor L. longipalpis takes its blood meal, compared with those from lymph node (6.3\/mm\u00b2 x 8.3\/mm\u00b2) and viscera (1.4\/mm\u00b2x 5.3\/mm\u00b2).<\/jats:p>","DOI":"10.1590\/s0036-46652010000500007","type":"journal-article","created":{"date-parts":[[2010,10,28]],"date-time":"2010-10-28T02:52:57Z","timestamp":1288234377000},"page":"259-266","source":"Crossref","is-referenced-by-count":23,"title":["Canine visceral leishmaniasis due to Leishmania (L.) infantum chagasi in Amazonian Brazil: comparison of the parasite density from the skin, lymph node and visceral tissues between symptomatic and asymptomatic, seropositive dogs"],"prefix":"10.1590","volume":"52","author":[{"given":"Luciana Vieira R.","family":"Lima","sequence":"first","affiliation":[{"name":"Instituto Evandro Chagas, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liliane Almeida","family":"Carneiro","sequence":"additional","affiliation":[{"name":"Instituto Evandro Chagas, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marliane Batista","family":"Campos","sequence":"additional","affiliation":[{"name":"Instituto Evandro Chagas, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Euj\u00eania Janis","family":"Chagas","sequence":"additional","affiliation":[{"name":"Secretaria de Sa\u00fade do Munic\u00edpio de Barcarena, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M\u00e1rcia D.","family":"Laurenti","sequence":"additional","affiliation":[{"name":"Universidade de S\u00e3o Paulo, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlos E.P.","family":"Corbett","sequence":"additional","affiliation":[{"name":"Universidade de S\u00e3o Paulo, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ralph","family":"Lainson","sequence":"additional","affiliation":[{"name":"Instituto Evandro Chagas, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fernando Tobias","family":"Silveira","sequence":"additional","affiliation":[{"name":"Instituto Evandro Chagas, Brasil; Universidade Federal do Par\u00e1, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"53","DOI":"10.4269\/ajtmh.1998.59.53","article-title":"Studies on control of visceral leishmaniasis: impact of dog control on canine and human visceral leishmaniasis in Jacobina, Bahia, Brazil","volume":"59","author":"Ashford DA","year":"1998","journal-title":"Am J Trop Med Hyg","ISSN":"http:\/\/id.crossref.org\/issn\/0002-9637","issn-type":"print"},{"key":"ref2","series-title":"Bioestat 4.0: aplica\u00e7\u00f5es estat\u00edsticas nas \u00e1reas das Ci\u00eancias Biol\u00f3gicas e M\u00e9dicas","author":"Ayres M","year":"2005"},{"key":"ref3","series-title":"The infectivity of dogs infected with Leishmania chagasi for Lutzomyia longipalpis is not related to clinical status or the humoral response of the animals: Abstract Book of the Third World Congress of Leishmaniasis","first-page":"110","author":"Barata IR","year":"2005"},{"key":"ref4","series-title":"Manual de Vigil\u00e2ncia e Controle da Leishmaniose visceral","year":"2003"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1590\/S0074-02761938000100010","article-title":"Leishmaniose visceral americana (Relat\u00f3rio dos trabalhos realizados pela Comiss\u00e3o Encarregada do Estudo da Leishmaniose Visceral Americana em 1937)","volume":"33","author":"Chagas E","year":"1938","journal-title":"Mem Inst Oswaldo Cruz","ISSN":"http:\/\/id.crossref.org\/issn\/0074-0276","issn-type":"print"},{"issue":"44","key":"ref6","doi-asserted-by":"crossref","first-page":"993","DOI":"10.1007\/BF02235488","article-title":"Cell-mediated immune response in megacolon from patients with chronic Chagas disease","author":"Corbett CEP","year":"2001","journal-title":"Dis Colon Rectum","ISSN":"http:\/\/id.crossref.org\/issn\/0012-3706","issn-type":"print"},{"key":"ref7","first-page":"141","article-title":"Toxoplasmose ocular - utiliza\u00e7\u00e3o de rea\u00e7\u00e3o imuno-histoqu\u00edmica para diagn\u00f3stico","volume":"51","author":"Corr\u00eaa MC","year":"1996","journal-title":"Rev Hosp Cl\u00edn Fac Med S\u00e3o Paulo"},{"key":"ref8","series-title":"Topley & Wilson's Microbiology and microbial infections: Volume 5: Parasitolology","first-page":"57","volume-title":"Immunology and immunopathology of human parasitic infections","author":"Cox FEG","year":"1998","edition":"9"},{"key":"ref9","first-page":"323","article-title":"Compara\u00e7\u00e3o das t\u00e9cnicas de RIFI (ag. 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