{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,4,4]],"date-time":"2022-04-04T03:29:29Z","timestamp":1649042969854},"reference-count":11,"publisher":"FapUNIFESP (SciELO)","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rev. Inst. Med. trop. S. Paulo"],"published-print":{"date-parts":[[2011,8]]},"abstract":"<jats:p>An autochthonous case of visceral leishmaniasis is reported in a dog (Canis familiaris) as an apparently natural infection in a non-endemic area. DNA obtained from spleen and liver samples produced the expected fragment in a Leishmania-specific rDNA-based nested-PCR assay. The PCR product, a 490 bp fragment, was sequenced and the nucleotide sequence was identical to that of Leishmania (Leishmania) infantum chagasi. These results are surprising since no autochthonous human or canine cases of visceral leishmaniasis have ever been reported in this municipality. This case suggests that natural transmission of this disease is occurring in this area.<\/jats:p>","DOI":"10.1590\/s0036-46652011000400010","type":"journal-article","created":{"date-parts":[[2011,9,8]],"date-time":"2011-09-08T16:16:15Z","timestamp":1315498575000},"page":"227-229","source":"Crossref","is-referenced-by-count":4,"title":["First occurrence of an autochthonous canine case of Leishmania (Leishmania) infantum chagasi in the municipality of Campinas, State of S\u00e3o Paulo, Brazil"],"prefix":"10.1590","volume":"53","author":[{"given":"Elisa San Martin Mouriz","family":"Savani","sequence":"first","affiliation":[{"name":"Centro de Controle de Zoonoses do Munic\u00edpio de S\u00e3o Paulo,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Douglas","family":"Presotto","sequence":"additional","affiliation":[{"name":"Centro de Controle de Zoonoses do Munic\u00edpio de Campinas,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thais","family":"Roberto","sequence":"additional","affiliation":[{"name":"Hospital Veterin\u00e1rio Taquaral,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Cec\u00edlia Gibrail de Oliveira","family":"Camargo","sequence":"additional","affiliation":[{"name":"Centro de Controle de Zoonoses do Munic\u00edpio de S\u00e3o Paulo,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandra Regina Nicoletti","family":"D'auria","sequence":"additional","affiliation":[{"name":"Centro de Controle de Zoonoses do Munic\u00edpio de S\u00e3o Paulo,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D\u00e9bora Veiga","family":"Sacramento","sequence":"additional","affiliation":[{"name":"Genomic Engenharia Molecular,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"540","DOI":"10.1128\/JCM.41.2.540-546.2003","article-title":"New PCR assay using glucose-6-phosphate dehydrogenase for the identification of Leishmania species","volume":"41","author":"Castilho TM","year":"2003","journal-title":"J Clin Microbiol","ISSN":"http:\/\/id.crossref.org\/issn\/0095-1137","issn-type":"print"},{"issue":"5","key":"ref2","first-page":"18","article-title":"Atualiza\u00e7\u00e3o da classifica\u00e7\u00e3o epidemiol\u00f3gica dos munic\u00edpios para Leishmaniose Visceral Americana","year":"2008","journal-title":"Bol Epidemiol Paul"},{"key":"ref3","article-title":"Leishmaniose visceral americana no Estado de S\u00e3o Paulo","volume":"32","author":"Galimbertti MZ","year":"1999","journal-title":"Rev Soc Bras Med Trop","ISSN":"http:\/\/id.crossref.org\/issn\/0037-8682","issn-type":"print"},{"key":"ref4","first-page":"95","article-title":"BioEdit: a user friendly biological sequence alignment editor and analysis program for Windows 95\/98\/NT","volume":"41","author":"Hall T A","year":"1999","journal-title":"Nucl Acids Symp Ser"},{"key":"ref5","first-page":"166","article-title":"Autochthonous visceral leishmaniasis in dogs of Embu-das-Artes, S\u00e3o Paulo","volume":"45","author":"Savani ES","year":"2003","journal-title":"Rev Inst Med Trop Sao Paulo","ISSN":"http:\/\/id.crossref.org\/issn\/0036-4665","issn-type":"print"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1016\/j.vetpar.2004.01.008","article-title":"The first record in the Americas of an autochthonous case of Leishmania (Leishmania) infantum chagasi in a domestic cat (Felix catus) from Cotia County, Sao Paulo State, Brazil","volume":"120","author":"Savani ES","year":"2004","journal-title":"Vet Parasitol","ISSN":"http:\/\/id.crossref.org\/issn\/0304-4017","issn-type":"print"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"739","DOI":"10.1590\/S0074-02762005000700011","article-title":"Occurrence of co-infection by Leishmania (Leishmania) chagasi and Trypanosoma (Trypanozoon) evansi in a dog in the state of Mato Grosso do Sul, Brazil","volume":"100","author":"Savani ES","year":"2005","journal-title":"Mem Inst Oswaldo Cruz","ISSN":"http:\/\/id.crossref.org\/issn\/0074-0276","issn-type":"print"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1016\/j.vetpar.2008.10.090","article-title":"The finding of Lutzomyia almerioi and Lutzomyia longipalpis naturally infected by Leishmania spp. in a cutaneous and canine visceral leishmaniases focus in Serra da Bodoquena, Brazil","volume":"160","author":"Savani ES","year":"2009","journal-title":"Vet Parasitol","ISSN":"http:\/\/id.crossref.org\/issn\/0304-4017","issn-type":"print"},{"issue":"77","key":"ref9","first-page":"21","article-title":"Classifica\u00e7\u00e3o epidemiol\u00f3gica dos munic\u00edpios segundo o Programa de Vigil\u00e2ncia e Controle da Leishmaniose Visceral Americana no Estado de S\u00e3o Paulo, atualizado em maio de 2010","volume":"7","year":"2010","journal-title":"Bol. 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