{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T23:01:34Z","timestamp":1792796494874,"version":"4.5.0"},"reference-count":42,"publisher":"FapUNIFESP (SciELO)","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rev. Inst. Med. trop. S. Paulo"],"published-print":{"date-parts":[[2012,4]]},"abstract":"<jats:p>This study examined the susceptibility of peritoneal macrophage (PM) from the Neotropical primates: Callithrix jacchus, Callithrix penicillata, Saimiri sciureus, Aotus azarae infulatus and Callimico goeldii to ex vivo Leishmania (L.) infantum chagasi-infection, the etiological agent of American visceral leishmaniasis (AVL), as a screening assay for evaluating the potential of these non-human primates as experimental models for studying AVL. The PM-susceptibility to infection was accessed by the PM-infection index (PMI) at 24, 72 h and by the mean of these rates (FPMI), as well as by the TNF-\u03b1, IL-12 (Capture ELISA) and Nitric oxide (NO) responses (Griess method). At 24h, the PMI of A. azarae infulatus (128) was higher than those of C. penicillata (83), C. goeldii (78), S. sciureus (77) and C. jacchus (55). At 72h, there was a significant PMI decrease in four monkeys: A. azarae infulatus (128\/37), C. penicillata (83\/38), S. sciureus (77\/38) and C. jacchus (55\/12), with exception of C. goeldii (78\/54). The FPMI of A. azarae infulatus (82.5) and C. goeldii (66) were higher than C. jacchus (33.5), but not higher than those of C. penicillata (60.5) and S. sciureus (57.5). The TNF-a response was more regular in those four primates which decreased their PMI at 24\/72 h: C. jacchus (145\/122 pg\/mL), C. penicillata (154\/130 pg\/mL), S. sciureus (164\/104 pg\/mL) and A. azarae infulatus (154\/104 pg\/mL), with exception of C. goeldii (38\/83 pg\/mL). The IL-12 response was mainly prominent in A. infulatus and C. goeldii which presented the highest FPMI and, the NO response was higher in C. goeldii, mainly at 72 h. These findings strongly suggest that these New World primates have developed a resistant innate immune response mechanism capable of controlling the macrophage intracellular growth of L. (L.) i. chagasi-infection, which do not encourage their use as animal model for studying AVL.<\/jats:p>","DOI":"10.1590\/s0036-46652012000200007","type":"journal-article","created":{"date-parts":[[2012,4,11]],"date-time":"2012-04-11T11:46:06Z","timestamp":1334144766000},"page":"95-102","source":"Crossref","is-referenced-by-count":10,"title":["Susceptibility of peritoneal macrophage from different species of neotropical primates to Ex vivo Leishmania (L.) infantum chagasi-infection"],"prefix":"10.1590","volume":"54","author":[{"given":"Liliane Almeida","family":"Carneiro","sequence":"first","affiliation":[{"name":"Ministry of Health, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00e1rcia Dalastra","family":"Laurenti","sequence":"additional","affiliation":[{"name":"S\u00e3o Paulo University, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marliane Batista","family":"Campos","sequence":"additional","affiliation":[{"name":"Ministry of Health, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia Maria de Castro","family":"Gomes","sequence":"additional","affiliation":[{"name":"S\u00e3o Paulo University, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos Eduardo Pereira","family":"Corbett","sequence":"additional","affiliation":[{"name":"S\u00e3o Paulo University, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernando Tobias","family":"Silveira","sequence":"additional","affiliation":[{"name":"Ministry of Health, Brazil; Federal University of Par\u00e1, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"530","reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1046\/j.1365-3083.2002.01101.x","article-title":"IL-12: the role of p40 versus p75","volume":"56","author":"Abdi K","year":"2012","journal-title":"Scand J 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