{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T03:18:50Z","timestamp":1787023130323,"version":"build-2736575974"},"reference-count":35,"publisher":"FapUNIFESP (SciELO)","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rev. Inst. Med. trop. S. Paulo"],"published-print":{"date-parts":[[2012,6]]},"abstract":"<jats:p>At first Rickettsia conorii was implicated as the causative agent of spotted fever in Uruguay diagnosed by serological assays. Later Rickettsia parkeri was detected in human-biting Amblyomma triste ticks using molecular tests. The natural vector of R. conorii, Rhipicephalus sanguineus, has not been studied for the presence of rickettsial organisms in Uruguay. To address this question, 180 R. sanguineus from dogs and 245 A. triste from vegetation (flagging) collected in three endemic localities were screened for spotted fever group (SFG) rickettsiosis in southern Uruguay. Tick extracted DNA pools were subjected to PCR using primers which amplify a fragment of the rickettsial gltA gene. Positive tick DNA pools with these primers were subjected to a second PCR round with primers targeting a fragment of the ompA gene, which is only present in SFG rickettsiae. No rickettsial DNA was detected in R. sanguineus. However, DNA pools of A. triste were found to be positive for a rickettsial organism in two of the three localities, with prevalences of 11.8% to 37.5% positive pools. DNA sequences generated from these PCR-positive ticks corresponded to R. parkeri. These findings, joint with the aggressiveness shown by A. triste towards humans, support previous data on the involvement of A. triste as vector of human infections caused by R. parkeri in Uruguay.<\/jats:p>","DOI":"10.1590\/s0036-46652012000300003","type":"journal-article","created":{"date-parts":[[2012,5,23]],"date-time":"2012-05-23T08:27:16Z","timestamp":1337761636000},"page":"131-134","source":"Crossref","is-referenced-by-count":17,"title":["Rickettsia parkeri: a Rickettsial pathogen transmitted by ticks in endemic areas for spotted fever rickettsiosis in southern Uruguay"],"prefix":"10.1590","volume":"54","author":[{"given":"Jos\u00e9 M.","family":"Venzal","sequence":"first","affiliation":[{"name":"Universidad de la Rep\u00fablica, Uruguay"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agust\u00edn","family":"Estrada-Pe\u00f1a","sequence":"additional","affiliation":[{"name":"Universidad de Zaragoza, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ar\u00e1nzazu","family":"Portillo","sequence":"additional","affiliation":[{"name":"Hospital San Pedro, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atilio J.","family":"Mangold","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Tecnolog\u00eda Agropecuaria, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oscar","family":"Castro","sequence":"additional","affiliation":[{"name":"Universidad de la Rep\u00fablica, Uruguay"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos G. De","family":"Souza","sequence":"additional","affiliation":[{"name":"Universidad de la Rep\u00fablica, Uruguay"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda L.","family":"F\u00e9lix","sequence":"additional","affiliation":[{"name":"Universidad de la Rep\u00fablica, Uruguay"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"P\u00e9rez-Mart\u00ednez","sequence":"additional","affiliation":[{"name":"Hospital San Pedro, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sonia","family":"Santib\u00e1nez","sequence":"additional","affiliation":[{"name":"Hospital San Pedro, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 A.","family":"Oteo","sequence":"additional","affiliation":[{"name":"Hospital San Pedro, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"530","reference":[{"key":"ref1","series-title":"Carrapatos de import\u00e2ncia m\u00e9dico-veterin\u00e1ria da Regi\u00e3o Neotropical: um guia ilustrado para identifica\u00e7\u00e3o de esp\u00e9cies","author":"Barros-Battesti DM","year":"2006"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"653","DOI":"10.1093\/jmedent\/35.5.653","article-title":"Minimum infection rate of Amblyomma americanum (Acari:Ixodidae) by Ehrlichia chaffeensis (Rickettsiales: Ehrlichieae) in southern Indiana","volume":"35","author":"Burket CT","year":"1998","journal-title":"J Med Entomol","ISSN":"https:\/\/id.crossref.org\/issn\/0022-2585","issn-type":"print"},{"key":"ref3","first-page":"8","article-title":"Primera detecci\u00f3n de Rickettsia massiliae en la ciudad de Buenos Aires: Resultado preliminares","volume":"1","author":"Cicuttin GL","year":"2004","journal-title":"Rev Argent Zoon"},{"key":"ref4","first-page":"119","article-title":"Rickettsiosis por Rickettsia conorii (fiebre botonosa del Mediterr\u00e1neo o fiebre de Marsella): Estado actual en Uruguay","volume":"17","author":"Conti-D\u00edaz 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Israeli spotted fever, Indian tick typhus, Astrakhan fever)","author":"Rovery C","year":"2007"},{"key":"ref24","first-page":"406","article-title":"The neighbor-joining method: a new method for reconstructing phylogenetic trees","volume":"4","author":"Saitou N","year":"1987","journal-title":"Mol Biol Evol","ISSN":"https:\/\/id.crossref.org\/issn\/0737-4038","issn-type":"print"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"1111","DOI":"10.3201\/eid1307.061397","article-title":"Rickettsia parkeri in Brazil","volume":"13","author":"Silveira I","year":"2007","journal-title":"Emerg Infect Dis","ISSN":"https:\/\/id.crossref.org\/issn\/1080-6040","issn-type":"print"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"521","DOI":"10.3201\/eid1603.091338","article-title":"Novel spotted fever group rickettsiosis, Brazil","volume":"16","author":"Spolidorio MG","year":"2010","journal-title":"Emerg Infect 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Evol","ISSN":"https:\/\/id.crossref.org\/issn\/0737-4038","issn-type":"print"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"953","DOI":"10.1089\/vbz.2009.0126","article-title":"Rickettsia infection in dogs and Rickettsia parkeri in Amblyomma tigrinum ticks, Cochabamba Department, Bolivia","volume":"10","author":"Tomassone L","year":"2010","journal-title":"Vector Borne Zoonotic Dis"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"769","DOI":"10.1179\/000349803225002327","article-title":"Ticks (Ixodida: Ixodidae) parasitising humans in Uruguay","volume":"97","author":"Venzal JM","year":"2003","journal-title":"Ann Trop Med Parasit","ISSN":"https:\/\/id.crossref.org\/issn\/0003-4983","issn-type":"print"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"1493","DOI":"10.3201\/eid1008.030999","article-title":"Rickettsia parkeri in Amblyomma triste from Uruguay","volume":"10","author":"Venzal JM","year":"2004","journal-title":"Emerg Infect 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Paulo"],"original-title":[],"link":[{"URL":"http:\/\/www.scielo.br\/pdf\/rimtsp\/v54n3\/03.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,7,7]],"date-time":"2020-07-07T20:49:59Z","timestamp":1594154999000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_arttext&pid=S0036-46652012000300003&lng=en&tlng=en"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,6]]},"references-count":35,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2012,6]]}},"alternative-id":["S0036-46652012000300003"],"URL":"https:\/\/doi.org\/10.1590\/s0036-46652012000300003","relation":{},"ISSN":["0036-4665"],"issn-type":[{"value":"0036-4665","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,6]]}}}