{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,3,29]],"date-time":"2022-03-29T01:58:19Z","timestamp":1648519099463},"reference-count":18,"publisher":"FapUNIFESP (SciELO)","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rev. Inst. Med. trop. S. Paulo"],"published-print":{"date-parts":[[2012,10]]},"abstract":"<jats:p>Schistosomiasis constitutes a major public health problem, with an estimated 200 million individuals infected worldwide and 700 million people living in risk areas. In Brazil there are areas of high, medium and low endemicity. Studies have shown that in endemic areas with a low prevalence of Schistosoma infection the sensitivity of parasitological methods is clearly reduced. Consequently diagnosis is often impeded due to the presence of false-negative results. The aim of this study is to present the PCR reamplification (Re-PCR) protocol for the detection of Schistosoma mansoni in samples with low parasite load (with less than 100 eggs per gram (epg) of feces). Three methods were used for the lysis of the envelopes of the S. mansoni eggs and two techniques of DNA extraction were carried out. Extracted DNA was quantified, and the results suggested that the extraction technique, which mixed glass beads with a guanidine isothiocyanate\/phenol\/chloroform (GT) solution, produced good results. PCR reamplification was conducted and detection sensitivity was found to be five eggs per 500 mg of artificially marked feces. The results achieved using these methods suggest that they are potentially viable for the detection of Schistosoma infection with low parasite load.<\/jats:p>","DOI":"10.1590\/s0036-46652012000500002","type":"journal-article","created":{"date-parts":[[2012,10,1]],"date-time":"2012-10-01T18:58:14Z","timestamp":1349117894000},"page":"245-248","source":"Crossref","is-referenced-by-count":8,"title":["Two sequential PCR amplifications for detection of Schistosoma mansoni in stool samples with low parasite load"],"prefix":"10.1590","volume":"54","author":[{"given":"Maria Cristina Carvalho do","family":"Esp\u00edrito-Santo","sequence":"first","affiliation":[{"name":"University of S\u00e3o Paulo,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M\u00f3nica Viviana","family":"Alvarado-Mora","sequence":"additional","affiliation":[{"name":"University of S\u00e3o Paulo,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pedro Luiz Silva","family":"Pinto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Flair Jos\u00e9","family":"Carrilho","sequence":"additional","affiliation":[{"name":"University of S\u00e3o Paulo,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jo\u00e3o Renato Rebello","family":"Pinho","sequence":"additional","affiliation":[{"name":"University of S\u00e3o Paulo,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ronaldo Cesar Borges","family":"Gryschek","sequence":"additional","affiliation":[{"name":"University of S\u00e3o Paulo,  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","reference":[{"key":"ref1","series-title":"Doen\u00e7as infecciosas e parasit\u00e1rias: guia de bolso","first-page":"169","volume-title":"Esquistossomose Mansoni","year":"2010","edition":"8"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/S0001-706X(00)00122-4","article-title":"The global status of schistosomiasis and its control","volume":"77","author":"Chitsulo L","year":"2000","journal-title":"Acta Trop","ISSN":"http:\/\/id.crossref.org\/issn\/0001-706X","issn-type":"print"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1016\/0003-2697(87)90021-2","article-title":"Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction","volume":"162","author":"Chomczynski P","year":"1987","journal-title":"Anal Biochem","ISSN":"http:\/\/id.crossref.org\/issn\/0003-2697","issn-type":"print"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"e32","DOI":"10.1371\/journal.pntd.0000032","article-title":"A schistosomiasis research agenda","volume":"1","author":"Colley DG","year":"2007","journal-title":"PLoS Negl Trop Dis"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/S1084-8592(99)80050-2","article-title":"Fast and reliable extraction of protozoan parasite DNA from fecal specimens","volume":"4","author":"Da Silva AJ","year":"1999","journal-title":"Mol Diagn","ISSN":"http:\/\/id.crossref.org\/issn\/1084-8592","issn-type":"print"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/S0169-4758(97)01204-0","article-title":"A vaccine for schistosomiasis: alternative approaches","volume":"14","author":"Doenhoff MJ","year":"1998","journal-title":"Parasitol Today","ISSN":"http:\/\/id.crossref.org\/issn\/0169-4758","issn-type":"print"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"450","DOI":"10.2307\/3280573","article-title":"A sieving method for the collection of schistosome eggs from mouse intestines","volume":"67","author":"Dresden MH","year":"1981","journal-title":"J Parasitol","ISSN":"http:\/\/id.crossref.org\/issn\/0022-3395","issn-type":"print"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1101\/gr.8.3.186","article-title":"Base-calling of automated sequencer traces using phred II. 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