{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T13:28:45Z","timestamp":1789910925435,"version":"4.0.1"},"reference-count":17,"publisher":"FapUNIFESP (SciELO)","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rev. Inst. Med. trop. S. Paulo"],"published-print":{"date-parts":[[2014,4]]},"abstract":"<jats:p>Objective: To assess quantitative real-time polymerase chain reaction (q-PCR) for the sputum smear diagnosis of pulmonary tuberculosis (PTB) in patients living with HIV\/AIDS with a clinical suspicion of PTB.Method: This is a prospective study to assess the accuracy of a diagnostic test, conducted on 140 sputum specimens from 140 patients living with HIV\/AIDS with a clinical suspicion of PTB, attended at two referral hospitals for people living with HIV\/AIDS in the city of Recife, Pernambuco, Brazil. A L\u00f6wenstein-Jensen medium culture and 7H9 broth were used as gold standard.Results: Of the 140 sputum samples, 47 (33.6%) were positive with the gold standard. q-PCR was positive in 42 (30%) of the 140 patients. Only one (0.71%) did not correspond to the culture. The sensitivity, specificity and accuracy of the q-PCR were 87.2%, 98.9% and 95% respectively. In 39 (93%) of the 42 q-PCR positive cases, the CT (threshold cycle) was equal to or less than 37.Conclusion: q-PCR performed on sputum smears from patients living with HIV\/AIDS demonstrated satisfactory sensitivity, specificity and accuracy, and may therefore be recommended as a method for diagnosing PTB.<\/jats:p>","DOI":"10.1590\/s0036-46652014000200009","type":"journal-article","created":{"date-parts":[[2014,3,26]],"date-time":"2014-03-26T15:39:53Z","timestamp":1395848393000},"page":"139-142","source":"Crossref","is-referenced-by-count":10,"title":["QUANTITATIVE REAL-TIME PCR (Q-PCR) FOR SPUTUM SMEAR DIAGNOSIS OF PULMONARY TUBERCULOSIS AMONG PEOPLE WITH HIV\/AIDS"],"prefix":"10.1590","volume":"56","author":[{"given":"Yvana Maria Maia de","family":"Albuquerque","sequence":"first","affiliation":[{"name":"Universidade Federal de Pernambuco,  Brazil; Correia Pican\u00e7o Hospital- State Secretariat of Health,  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Luiza Magalh\u00e3es de Andrade","family":"Lima","sequence":"additional","affiliation":[{"name":"Faculdade Pernambucana de Sa\u00fade,  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Kelly","family":"Lins","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio Marcelo Magalh\u00e3es,  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcelo","family":"Magalh\u00e3es","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio Marcelo Magalh\u00e3es,  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vera","family":"Magalh\u00e3es","sequence":"additional","affiliation":[{"name":"Universidade Federal de Pernambuco,  Brazil; Laborat\u00f3rio Marcelo Magalh\u00e3es,  Brazil; Universidade Federal de Pernambuco-UFPE,  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"530","reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"1010","DOI":"10.1056\/NEJMoa0907847","article-title":"Rapid molecular detection of tuberculosis and rifampin resistance","volume":"363","author":"Boehme CC","year":"2010","journal-title":"N Engl J Med"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"2238","DOI":"10.1164\/ajrccm.162.6.2003125","article-title":"Comparison of sputum induction with fiberoptic bronchoscopy in the diagnosis of tuberculosis: experience at an acquired imumune deficiency syndrome reference center in Rio de Janeiro, Brazil","volume":"162","author":"Conde MB","year":"2000","journal-title":"Am J Respir Crit Care Med"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"698","DOI":"10.1590\/S0102-311X2012000400009","article-title":"Hepatotoxicity induced by antituberculosis drugs among patients coinfect with HIV and 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high prevalences of tuberculosis and human immunodeficiency virus","volume":"42","author":"Kivihya-Ndugga L","year":"2004","journal-title":"J Clin Microbiol"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"4307","DOI":"10.1128\/JCM.42.9.4307-4309.2004","article-title":"Comparison of the real time PCR method and the Gen-Probe amplified Mycobacterium tuberculosis direct test for detection of Mycobacterium tuberculosis in pulmonary and nonpulmonary specimens","volume":"42","author":"Lemaitre N","year":"2004","journal-title":"J Clin Microbiol"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"1364","DOI":"10.1111\/j.1365-2672.2009.04324.x","article-title":"Development of a simple and a low-cost real-time PCR method for the identification of commonly encountered mycobacteria in a high throughput laboratory","volume":"107","author":"Leung KI","year":"2009","journal-title":"J Appl Microbiol"},{"key":"ref12","first-page":"1313","article-title":"Coinfect tuberculosis and HIVAIDS","author":"Melo FAF","year":"2009","journal-title":"Veronesi R, Focaccia R, editors Treaty of infectology. 4th ed"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"850","DOI":"10.4103\/0971-5916.92630","article-title":"Diagnosis and treatment of tuberculosis in HIV co-infected patients","volume":"134","author":"Padmapriyadarsini C","year":"2011","journal-title":"Indian J Med Res"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1016\/0962-8479(95)90583-9","article-title":"The use of sputum induction for establishing a diagnosis in patients with suspected pulmonary tuberculosis in Malawi","volume":"76","author":"Parry CM","year":"1995","journal-title":"Tuber Lung Dis"},{"issue":"(Suppl 1)","key":"ref15","first-page":"57","article-title":"Improving diagnosis of pulmonary tuberculosis among HIV\/AIDS patients: literature review and experience in teaching hospital in Indonesia","volume":"41","author":"Rewata L","year":"2009","journal-title":"Acta 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