{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,5,16]],"date-time":"2024-05-16T06:28:44Z","timestamp":1715840924941},"reference-count":42,"publisher":"FapUNIFESP (SciELO)","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rev. Inst. Med. trop. S. Paulo"],"published-print":{"date-parts":[[2015,2]]},"abstract":"<jats:p>Introduction: The objective of this study was to determine the prevalence and etiological profile of enteropathogens in children from a daycare center. Methods: From October 2010 to February 2011 stool samples from 100 children enrolled in a government daycare center in the municipality of S\u00e3o Jos\u00e9 do Rio Preto, in the state of S\u00e3o Paulo, were collected and analyzed. Results: A total of 246 bacteria were isolated in 99% of the fecal samples; 129 were in the diarrheal group and 117 in the non-diarrheal group. Seventy-three strains of Escherichia coli were isolated, 19 of Enterobacter, one of Alcaligenes and one of Proteus. There were 14 cases of mixed colonization with Enterobacter and E. coli. Norovirus and Astrovirus were detected in children with clinical signs suggestive of diarrhea. These viruses were detected exclusively among children residing in urban areas. All fecal samples were negative for the presence of the rotavirus species A and C. The presence of Giardia lamblia, Entamoeba coli, Endolimax nana and hookworm was observed. A significant association was found between food consumption outside home and daycare center and the presence of intestinal parasites. Conclusions: For children of this daycare center, intestinal infection due to pathogens does not seem to have contributed to the occurrence of diarrhea or other intestinal symptoms. The observed differences may be due to the wide diversity of geographical, social and economic characteristics and the climate of Brazil, all of which have been reported as critical factors in the modulation of the frequency of different enteropathogens.<\/jats:p>","DOI":"10.1590\/s0036-46652015000100004","type":"journal-article","created":{"date-parts":[[2015,1,28]],"date-time":"2015-01-28T19:36:06Z","timestamp":1422473766000},"page":"27-32","source":"Crossref","is-referenced-by-count":14,"title":["ENTEROPATHOGENS DETECTED IN A DAYCARE CENTER, SOUTHEASTERN BRAZIL: BACTERIA, VIRUS, AND PARASITE RESEARCH"],"prefix":"10.1590","volume":"57","author":[{"given":"Edna Donizetti Rossi","family":"Castro","sequence":"first","affiliation":[{"name":"Sao Jose do Rio Preto Faculty of Medicine, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcela Cristina Braga Yassaka","family":"Germini","sequence":"additional","affiliation":[{"name":"Sao Jose do Rio Preto Faculty of Medicine, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joana D'Arc Pereira","family":"Mascarenhas","sequence":"additional","affiliation":[{"name":"Evandro Chagas Institute, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yvone Benchimol","family":"Gabbay","sequence":"additional","affiliation":[{"name":"Evandro Chagas Institute, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ian Carlos Gomes de","family":"Lima","sequence":"additional","affiliation":[{"name":"Evandro Chagas Institute, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patr\u00edcia dos Santos","family":"Lobo","sequence":"additional","affiliation":[{"name":"Evandro Chagas Institute, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Val\u00e9ria Daltibari","family":"Fraga","sequence":"additional","affiliation":[{"name":"Sao Jose do Rio Preto Faculty of Medicine, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luciana Moran","family":"Concei\u00e7\u00e3o","sequence":"additional","affiliation":[{"name":"Sao Jose do Rio Preto Faculty of Medicine, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ricardo Luiz Dantas","family":"Machado","sequence":"additional","affiliation":[{"name":"Sao Jose do Rio Preto Faculty of Medicine, Brazil; Evandro Chagas Institute, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andr\u00e9a Regina Baptista","family":"Rossit","sequence":"additional","affiliation":[{"name":"Sao Jose do Rio Preto Faculty of Medicine, Brazil; Biomedical Institute of the Fluminense Federal University, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1128\/CMR.14.3.447-475.2001","article-title":"Biology of <em>Giardia lamblia<\/em>","volume":"14","author":"Adam RD","year":"2001","journal-title":"Clin Microbiol Rev"},{"key":"ref2","first-page":"291","article-title":"Enteropat\u00f3genos associados com diarr\u00e9ia aguda em crian\u00e7as","volume":"74","author":"Almeida MTG","year":"1998","journal-title":"J Pediatr (Rio J)"},{"key":"ref3","first-page":"31","article-title":"Enteroparasitoses em escolares residentes na periferia de Porto Alegre, RS, Brasil","volume":"35","author":"Bencke A","year":"2006","journal-title":"Rev Patol Trop"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1128\/JCM.28.3.495-503.1990","article-title":"Rapid and simple method for purification of nucleic acids","volume":"28","author":"Boom R","year":"1990","journal-title":"J Clin Microbiol"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1053\/jinf.2002.1002","article-title":"Diarrhea, CD4 counts and enteric infections in a community based cohort of HIV-infected adults in Uganda","volume":"45","author":"Brink AK","year":"2002","journal-title":"J Infect"},{"key":"ref6","first-page":"CR624","article-title":"Detection and genotyping of astroviruses fro m children with acute gastroenteritis from Goiania, Goias, Brazil","volume":"8","author":"Cardoso D","year":"2002","journal-title":"Med Sci Monit"},{"key":"ref7","first-page":"25","article-title":"Frequ\u00eancia e aspectos epidemiol\u00f3gicos da giard\u00edase em creches do munic\u00edpio de Aracaju, SE, Brasil","volume":"28","author":"Cardoso GS","year":"1995","journal-title":"Rev Soc Bras Med Trop"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1590\/S0036-46652006000500006","article-title":"Occurrence of enteroparasites in day care centers in Botucatu (S\u00e3o Paulo State, Brazil) with emphasis on <em>Cryptosporidium<\/em> sp., <em>Giardia duodenalis<\/em> and <em>Enterobius vermicularis<\/em>","volume":"48","author":"Carvalho TB","year":"2006","journal-title":"Rev Inst Med Trop Sao Paulo"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"4266","DOI":"10.1128\/JCM.40.11.4266-4272.2002","article-title":"Virus diversity in a winter epidemic of acute diarrhea in France","volume":"40","author":"Chikhi- Brachet R","year":"2002","journal-title":"J Clin Microbiol"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1002\/jmv.10139","article-title":"Detection and characterisation of human astroviruses in children with acute gastroenteritis in Blantyre, Malawi","volume":"67","author":"Cunliffe N A","year":"2002","journal-title":"J Med Virol"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"1038","DOI":"10.1097\/00006454-200211000-00012","article-title":"Astrovirus acute gastroenteritis among child ren in Madrid, Spain","volume":"21","author":"Dalton RM","year":"2002","journal-title":"Pediatr Infect Dis J"},{"key":"ref12","author":"Fagundes U","year":"2010"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"241","DOI":"10.2307\/3272508","article-title":"Comparative efficiency of various techniques for the diagnosis of protozoa and helminthes in feces","volume":"25","author":"Faust EC","year":"1939","journal-title":"J Parasitol"},{"issue":"(6 Suppl)","key":"ref14","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1590\/S0034-89102000000700010","article-title":"Tend\u00eancia secular das parasitoses intestinais na inf\u00e2ncia na cidade de S\u00e3o Paulo (1984-1996)","volume":"34","author":"Ferreira MU","year":"2000","journal-title":"Rev Saude Publica"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"1666","DOI":"10.1002\/jmv.21250","article-title":"Evidence for zoonotic transmission of group C rotaviruses among children in Bel\u00e9m, Brazil","volume":"80","author":"Gabbay YB","year":"2008","journal-title":"J Med Virol"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"709","DOI":"10.1590\/S0074-02762005000700004","article-title":"Prevalence and genetic diversity of astroviruses in children with and without diarrhea in S\u00e3o Lu\u00eds, Maranh\u00e3o, Brazil","volume":"100","author":"Gabbay YB","year":"2005","journal-title":"Mem Inst Oswaldo Cruz"},{"key":"ref17","first-page":"69","article-title":"An outbreak of group C rotavirus gastroenteritis among children attending a day-care center in Belem. 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